( PDF ) Análise da comunidade bacteriana presente em fluidos produzidos na indústria do Petróleo

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DIOGO BASTOS SOUZA

Análise da Comunidade Bacteriana Presente em

Fluidos Produzidos na Indústria do Petróleo

Orientador: Lucy Seldin

UNIVERSIDADE FEDERAL DO RIO DE JANEIRO

INSTITUTO DE MICROBIOLOGIA PROF PAULO DE GÓES

RIO DE JANEIRO

NOVEMBRO DE 2009

Dissertação de Mestrado apresentada ao Programa de Pós-

Graduação em Ciências (Microbiologia), Instituto de

Microbiologia Prof. Paulo de Góes da Universidade Federal do

Rio de Janeiro, como parte dos requisitos necessários à

obtenção do título de Mestre em Ciências Biológicas

(Microbiologia)

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FICHA CATALOGRÁFICA

SOUZA, Diogo Bastos

Análise da Comunidade Bacteriana Presente em Fluidos Produzidos na Indústria do

Petróleo. / Diogo Bastos Souza - Rio de Janeiro, 2009.

xii 146p

Dissertação (Mestrado em Ciências Biológicas)

Universidade Federal do Rio de Janeiro/ Instituto de Microbiologia Prof. Paulo de Góes,

2009.

Orientador: Lucy Seldin

Referências bibliográficas: f 46

1. Diversidade. 2. Petróleo. 3. Bactéria. 4. Degradação. 5. Água Produzida. 6. Ecologia

Microbiana. I. Lucy Seldin II. UFRJ, Instituto de Microbiologia Prof. Paulo de Góes,

Mestrado em Ciências Biológicas. III. Análise da Comunidade Bacteriana Presente em

Fluidos Produzidos na Indústria do Petróleo.

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iii

Este trabalho foi realizado no

Laboratório de Genética Microbiana

do Departamento de Microbiologia

Geral do Instituto de Microbiologia

Prof. Paulo de Góes, UFRJ, sob a

orientação da Profa. Lucy Seldin.

AGRADECIMENTOS

A Deus, por mais uma oportunidade, pela conclusão de mais uma etapa

da vida e pela ajuda nos momentos em que mais ninguém podia ajudar.

Aos meus pais, pelo apoio incondicional a todas as decisões que tomei

na minha vida e por me fazer acreditar que os sacrifícios são necessários e que

valem à pena.

À Joelma, que me conheceu no meio desta trajetória, mas que foi muito

importante para a conclusão desta etapa.

À Professora Lucy, pelo apoio, pela paciência, pela oportunidade e por

todo conhecimento adquirido ao longo de todos esses anos.

À família do Laboratório de Genética Microbiana, pela ajuda no projeto,

por toda contribuição intelectual que me deram, por todos os churrascos, por

todas as festinhas e por todos os momentos bons que passamos durante esses

quase 6 anos que fizeram toda diferença na minha vida.

Aos Amigos Diogo e Joana, com os quais tive momentos inesquecíveis

de reflexão e de muita alegria.

A todos os professores e à Coordenação de Pós-Graduação, que

participaram dessa conquista.

À Petrobras, pelo financiamento do projeto e pela bolsa concedida.

DEDICATÓRIA

Dedico este trabalho a todas as pessoas que direta ou indiretamente me

ajudaram. A todas as que abriram mão dos próprios compromissos e doaram

seu tempo para estar comigo quando eu precisei. A essas pessoas que fizeram

muito por mim e só pediram que eu conseguisse concluir esse trabalho. Essas

pessoas são: meus pais (Jorge Carvalho e Vilma), o pai que eu escolhi (Jorge

Pereira), minha esposa (Joelma), meus irmãos (André e Itran), meus amigos

(Diogo Jurelevicius, Rodrigo Sá Freire, Leandro Valério, Lívia Melo e Leonardo

Santos), a minha orientadora Lucy Seldin que foi a pessoa que mais contribuiu

diretamete para que esse trabalho acontecesse.

RESUMO

Diogo Bastos Souza

Análise da Comunidade Bacteriana Presente em Fluidos Produzidos na

Indústria do Petróleo

Orientador: Lucy Seldin

Resumo da Dissertação de Mestrado submetida ao Programa de Pós-

Graduação em Ciências (Microbiologia), Instituto de Microbiologia Prof. Paulo

de Góes da Universidade Federal do Rio de Janeiro, como parte dos requisitos

necessários para a obtenção do título de Mestre em Ciências Biológicas.

O fluido produzido na recuperação secundária do petróleo pode

apresentar diferentes parâmetros de qualidade (valores de BSW, do inglês

“Basic Sediment and Water”, porcentagem de água e sedimentos no petróleo)

e de salinidade. Campos maduros tendem a produzir cada vez mais água no

decorrer de sua vida útil, comprometendo a capacidade de escoamento do óleo

para as refinarias. Dentre os problemas relacionados ao aumento da

quantidade de água no fluido produzido podemos destacar a biodegradação

(pela introdução de microrganismos nesses sistemas), elevando a viscosidade

e acidez do óleo, bem como reduzindo seu grau API, fazendo com que os

custos de sua produção sejam elevados. Sendo assim, o objetivo geral desse

trabalho foi o estudo por análise molecular da comunidade bacteriana de

fluidos produzidos de duas plataformas (P48 e P43). Duas amostras com

diferentes valores de BSW foram coletadas de cada campo. Alterações na

comunidade bacteriana relacionadas ao valor de BSW foram analisadas por

PCR seguida da construção de bibliotecas de clones. Para tal, foram utilizados

iniciadores universais bacterianos baseados no gene que codifica o 16S rRNA.

As seqüências obtidas foram comparadas entre si (programa webLibshuff) e

com outras seqüências presentes no GenBanK (programa blastn). Além disso,

foi feito o isolamento de estirpes bacterianas diretamente das quatro amostras

de fluido, e estas foram posteriormente caracterizadas (morfologia celular e

teste de degradação de óleo cru). A classe Gammaproteobacteria predominou

em todas as amostras estudadas, sendo o grupo majoritário o gênero

vii

Pseudomonas. Quando as seqüências obtidas das amostras de P48 e P43

foram comparadas por testes estatísticos (Libshuff), a amostra P48A (BSW

60%) foi a única que foi significativamente diferente das outras amostras nos

testes estatísticos. Apesar da grande maioria dos clones serem relacionados

ao gênero Pseudomonas, o qual já foi relacionado à biodegradação do petróleo

por vários autores, os isolados se apresentaram na maioria como bastonetes

Gram-positivos. Quando esses diferentes isolados foram testados quanto à

capacidade de degradação de óleo cru, apenas uma estirpe apresentou

resultado positivo. O presente trabalho mostrou que nos fluidos produzidos das

duas plataformas foram encontradas bactérias, sendo uma delas

potencialmente importante na degradação de petróleo.

Palavras-chave: 1. Diversidade. 2. Petróleo. 3. Bactéria. 4. Degradação. 5.

Água Produzida. 6. Ecologia Microbiana.

Rio de Janeiro

Novembro de 2009

viii

ABSTRACT

Diogo Bastos Souza

Análise da Comunidade Bacteriana em Fluidos Produzidos na Indústria do

Petróleo

Orientador: Lucy Seldin

Resumo da Dissertação de Mestrado submetida ao Programa de Pós-

Graduação em Ciências (Microbiologia), Instituto de Microbiologia Prof. Paulo

de Góes da Universidade Federal do Rio de Janeiro, como parte dos requisitos

necessários para a obtenção do título de Mestre em Ciências Biológicas.

The fluid produced in the secondary recovery of oil can have different

quality parameters (BSW value, which means Basic Sediment and Water and

measures the amount of water in oil) and salinity. Mature fields tend to produce

more water during their lifetime, affecting the flow capacity of oil refineries.

Among the problems related to the increased amount of water produced in the

fluid, biodegradation (by the introduction of microorganisms in these systems)

can cause the increase of viscosity and acidity of the oil and the reduction of its

API grade. Therefore, biodegradation can interfere on the financial cost of oil

production. The aim of this study was to characterize by molecular methods the

bacterial communities present in the fluid produced in two platforms (P48 and

P43). Two samples with different values of BSW were collected from each

platform. Changes in bacterial community related to the value of BSW were

analyzed by PCR followed by the construction of clone libraries. For that, we

used universal primers based on the gene coding for 16S rRNA. The

sequences obtained were compared between libraries (webLibshuff) and with

other sequences present in GenBank (blastn program). The class

Gammaproteobacteria predominated in all samples studied, and the main group

found was Pseudomonas. When the sequences obtained from samples of P48

and P43 were compared by statistical tests (Libshuff), the sample P48A (BSW

60%) was the only one that was significantly different from other samples in

statistical tests. Fourteen isolates were also obtained from the different fluid

samples and most of them were characterized as Gram-positive rods, although

the majority of the sequences from the clone libraries were affiliated with

Pseudomonas. Only one strain was characterized as Gram-negative rod. When

these different isolates were tested for their ability to degrade crude oil, only one

strain showed a positive result. This study showed that the fluids produced from

two platforms bacteria were found, one being potentially important in the

degradation of oil.

Palavras-chave: 1. Diversidade. 2. Petróleo. 3. Bactéria. 4. Degradação. 5.

Água Produzida. 6. Ecologia Microbiana.

Rio de Janeiro

Novembro de 2009

ÍNDICE

RESUMO............................................................................................................vi

ABSTRACT.......................................................................................................viii

ABREVIATURAS...............................................................................................xii

1. INTRODUÇÃO................................................................................................1

1.1. O PETRÓLEO..............................................................................................1

1.2. FORMAÇÃO DO PETRÓLEO E OS COMPOSTOS DO

PETRÓLEO.........................................................................................................2

1.3. RECUPERAÇÃO SECUNDÁRIA DO PETRÓLEO: ÁGUA

PRODUZIDA.......................................................................................................4

1.4. MICRORGANISMOS DO PETRÓLEO........................................................5

1.5. MICRORGANISMOS DEGRADADORES DE HIDROCARBONETOS.......7

1.6. BIODEGRADAÇÃO MICROBIANA EM RESERVATÓRIOS

PETROLÍFEROS................................................................................................9

1.7. ESTUDO DA DIVERSIDADE MICROBIANA.............................................10

1.8. MÉTODOS DEPENDENTES DE CULTIVO PARA ESTUDO DA

DIVERSIDADE MICROBIANA..........................................................................11

1.9. MÉTODOS INDEPENDENTES DE CULTIVO PARA ESTUDO DA

DIVERSIDADE MICROBIANA..........................................................................13

2. JUSTIFICATIVA DO TRABAHO E OBJETIVOS GERAIS..........................15

3. OBJETIVOS ESPECÍFICOS.........................................................................16

4. METODOLOGIA............................... ...........................................................17

4.1. MEIOS DE CULTURA...............................................................................17

4.2. SOLUÇÕES...............................................................................................18

4.3.TAMPÕES...................................................................................................18

4.4. CARACTERIZAÇÃO GENOTÍPICA...........................................................19

4.5. ISOLAMENTO DAS ESTIRPES................................................................23

4.6. TESTE DE DEGRADAÇÃO DE ÓLEO......................................................24

5. RESULTADOS..............................................................................................25

6. DISCUSSÃO.................................................................................................38

7. CONCLUSÕES.............................................................................................45

8. REFERÊNCIAS............................................................................................46

ANEXO I............................................................................................................55

xii

ABREVIATURAS

API - Grau API - é uma escala hidrométrica idealizada pelo “American

Petroleum Institute – API”, juntamente com a “National Bureau of Standards” e

utilizada para medir a densidade relativa de líquidos

BRS - Bactérias Redutoras de Sulfato

BSW - do inglês “Basic Sediments and Water”

DNA – Ácido Desoxirribonucléico

dNTP – deoxinucleotídeo trifosfato

EDTA – Ácido etilenodiaminotetracético

HPAs – Hidrocarbonetos Policíclicos Aromáticos

NAT – do inglês “Total Acid Number”

PCR – “Polimerase Chain Reaction” (Reação em Cadeia da Polimerase)

RNA – Ácido Ribonucléico

RNase A – Ribonuclease A

rRNA – RNA ribossômico

SDS – Dodecil Sulfato de Sódio

TE – Tampão Tris/EDTA

TEB – Tampão Tris/EDTA/H

1. INTRODUÇÃO

1.1. O PETRÓLEO

O petróleo pode ser definido como uma mistura sólida, líquida e/ou

gasosa, de ocorrência natural, formada predominantemente por

hidrocarbonetos saturados e aromáticos, e outros compostos orgânicos.

Contém, freqüentemente, quantidades significativas de nitrogênio, enxofre e

oxigênio, além de pequenas quantidades de metais como níquel e vanádio,

entre outros (EGLINTON & MURPHY, 1969).

Um reservatório de petróleo, por definição, é formado por três

elementos: a rocha matriz, a rocha reservatório e a rocha impermeável. A rocha

matriz é a rocha na qual o petróleo cru é formado e a partir da qual,

posteriormente, o mesmo migra até encontrar um terreno apropriado para se

concentrar. Estes terrenos são denominados bacias sedimentares ou rochas

reservatórios, formadas por camadas ou lençóis porosos de areia, arenitos ou

calcários. É a permeabilidade das rochas reservatórios que irá permitir o

acúmulo de petróleo nos poros, formando jazidas. Normalmente, esta rocha

possui uma grande capacidade de estocar fluidos ricos em hidrocarbonetos. Ali

são encontrados gás natural, na parte mais alta, e petróleo e água nas partes

mais baixas (PLANCKAERT, 2005). A rocha reservatório funciona como uma

esponja, pois ela pode armazenar e expelir o fluido. Já o papel da rocha

impermeável é conter a migração dos hidrocarbonetos, permitindo assim a

formação dos reservatórios de petróleo. Por possuir uma densidade inferior a

das rochas que constituem o subsolo, o petróleo tende a migrar para a

superfície. Se no caminho para a superfície, através das camadas de rochas

adjacentes, o petróleo encontra uma estrutura geológica impermeável (uma

rocha selante), que detenha seu caminho fazendo o seu confinamento e

impedindo a sua migração, acaba se formando um reservatório de petróleo.

Esse processo ocorre lentamente, levando alguns milhares de anos

(PLANCKAERT, 2005).

1.2. FORMAÇÃO DO PETRÓLEO E OS COMPOSTOS DO PETRÓLEO

A hipótese mais aceita para a origem do petróleo é de que ele tenha sido

gerado a partir de matéria orgânica depositada junto com sedimentos (NEIVA,

1983; BARKER, 1984; TISSOT & WELTE, 1984). Nessa hipótese, para o início

dos processos que levam à formação do petróleo, a interação dos fatores

matéria orgânica, sedimento e condições termoquímicas apropriadas é

fundamental (THOMAS, 2001). Após a incorporação da matéria orgânica ao

sedimento no fundo de corpos aquosos como lagos e mares rasos, dá-se o

aumento de carga sedimentar e de temperatura, começando, então, a se

delinear o processo de geração de petróleo. Em temperaturas mais baixas (50-

65ºC), a matéria orgânica é transformada em querogênio pela ação

microbiológica e reações químicas (ciclização, condensação, polimerização).

Este processo é denominado diagênese (Figura 1) (TISSOT & WELTE, 1984).

O querogênio formado pode ser definido como matéria orgânica contida nos

depósitos sedimentares que, através de um processo de perda de hidrogênio,

apresenta um alto conteúdo de carbono e elevada massa molecular (PELET,

BEHAR & MONIN, 1985). O querogênio pode ser definido também como a

matéria orgânica insolúvel em solventes orgânicos (TISSOT & WELTE, 1984).

O petróleo é formado, a partir do querogênio, durante a catagênese

(Figura 1) através de reações de craqueamento termocatalítico,

descarboxilação e desproporcionamento (TISSOT & WELTE, 1984; THOMAS,

2001). A continuação do processo (metagênese) (Figura 1) propicia a quebra

das moléculas de hidrocarbonetos líquidos e sua transformação em gás leve.

Devido a variações da distribuição da matéria orgânica derivada de

plantas, animais e vida marinha nos sedimentos depositados, além da história

geológica de soterramento, erosão e fluxo térmico de cada rocha geradora, não

existe uma composição única para o petróleo. Assim, por exemplo, uma fonte

de matéria orgânica rica em restos de coberturas vegetais, ésteres de ácidos

de cadeias longas e álcoois de cadeias longas poderão produzir óleos com alto

conteúdo de ceras e alto ponto de fluidez (BARKER, 1984). Sendo assim, a

composição do óleo cru pode fornecer informações importantes sobre o

ambiente deposicional e a fonte de matéria orgânica original.

Como citado anteriormente, o petróleo é constituído principalmente por

hidrocarbonetos saturados e aromáticos e, em menor quantidade, de

compostos não hidrocarbonetos (compostos heteroatômicos). Os

hidrocarbonetos saturados possuem somente ligações simples entre os átomos

de carbono e podem ser distribuídos de forma linear (n-alcanos), ramificada

(iso e anteiso alcanos) e cíclica (cicloalcanos ou compostos naftênicos)

(SOLOMONS, 1983). Os hidrocarbonetos aromáticos são caracterizados pela

presença de um ou mais anéis benzênicos (também chamados de anéis

aromáticos), que são constituídos por ligações duplas e simples que se

alternam em anéis com seis átomos de carbono (benzenóides) (ATKINS,

2000).

Figura 1 – Esquema ger

al do processo de geração do petróleo (modificado de

TISSOT & WELTE, 1984).

Os outros compostos do petróleo, chamados de não-hidrocarbonetos,

são compostos heteroatômicos porque possuem, além de carbono e

hidrogênio, um ou mais átomos de nitrogênio, enxofre e/ou oxigênio; daí a

designação comum desses compostos como “NSO”. Compostos com estas

características estão presentes nas frações de asfaltenos e resinas do petróleo.

A densidade do óleo cru é geralmente medida em graus, de acordo com

a escala desenvolvida pelo Instituto Americano do Petróleo (API) (TSALIK &

SCHIFFRIN, 2005). Os óleos crus leves, médios e pesados são considerados

“óleos crus convencionais”. Alguns tipos de óleos crus podem ser misturados

para produzir a qualidade que interessa aos refinadores. Os óleos leves

geralmente são mais caros que os pesados, principalmente devido aos

produtos refinados gerados como a gasolina ou o combustível de aviação. O

óleo abaixo de 10º API é comumente conhecido como betume e requer

tratamento especial. O betume é extraído por escavação da areia, do arenito

ou de outras rochas sedimentares, enquanto que os óleos crus convencionais

são extraídos por perfuração. O betume passa por vários processos de

lavagem e tratamento para separar o conteúdo de óleo da areia, água e

minerais, e é então diluído com petróleo. Pelo fato de passar por esses

processos, o betume ficou conhecido como “óleo cru sintético” (TSALIK &

SCHIFFRIN, 2005).

1.3. RECUPERAÇÃO SECUNDÁRIA DO PETRÓLEO: ÁGUA PRODUZIDA

A primeira fase de produção, a recuperação primária do petróleo, se

caracteriza pela saída espontânea do produto até a superfície, impelido pela

pressão interna dos gases (poços surgentes). Entretanto, quando isso não

ocorre, é preciso usar equipamentos para promover a elevação artificial dos

fluidos. Para manter as condições de pressão na rocha reservatório,

fundamentais para a migração do petróleo para os poços, efetua-se uma

operação de injeção de água (do mar) nas camadas inferiores da rocha

reservatório, e/ou gás nas camadas superiores, que é denominada como

recuperação secundária. Dessa forma, durante o processo efetivo de extração

de petróleo e gás, grandes volumes de água com sólidos suspensos e

dissolvidos são também trazidos à superfície. Denominada “água produzida”,

esta água extraída geralmente contém várias substâncias altamente tóxicas,

incluindo metais pesados (tais como chumbo, zinco e mercúrio) e compostos

orgânicos voláteis (tais como benzeno e tolueno). Pode também conter

elevados níveis de sal (US EPA, 2000; TSALIK & SCHIFFRIN, 2005). O fluido

produzido pode conter diferentes valores de BSW (do inglês “Basic Sediments

and Water”) que corresponde à quantidade de água presente no fluido

(PLANCKAERT, 2005).

À medida que a vida econômica dos poços vai se esgotando, o volume

de água produzida pode exceder até dez vezes o volume de óleo produzido

(PLANCKAERT, 2005). Nos Estados Unidos, esse volume corresponde a

aproximadamente oito barris de água para cada barril de petróleo produzido. A

quantidade de água produzida é menor para poços de gás natural (US EPA,

2000).

A composição da água produzida fornece informação relevante sobre o

reservatório de petróleo, determina a necessidade do uso de aditivos

emulsificantes e anticorrosivos e interfere no desenho de manejo e de sistemas

de tratamento da água. O tipo e a abundância dos constituintes dissolvidos na

água produzida (sais e outros compostos) variam dentro e entre bacias

geológicas (COLLINS, 1985; DALY & MESING, 1995). A variabilidade é o

resultado de reações geoquímicas entre a água e a rocha, bem como da

história hidrológica e geológica (LAND & PREZBINDOWSKI, 1981; STUEBER,

SALLER & ISHIDA, 1998).

O conhecimento a respeito comunidade bacteriana encontrada na água

produzida é de extrema importância para entender os processos que ocorrem

nos reservatórios de petróleo, pois esses microrganismos são injetados nos

reservatórios juntamente com a água e passam a causar diversas mudanças

nestes ambientes. Os principais gêneros de bactérias encontrados na água

produzida são: Achromobacter, Burkholderia, Pseudomonas, Marinobacter e

Colwellia (KORENBLUM et al., 2010).

1.4. MICRORGANISMOS DO PETRÓLEO

As comunidades naturais de microrganismos presentes em diferentes

habitats possuem uma grande versatilidade metabólica. Os microrganismos

são capazes de metabolizar e freqüentemente mineralizar uma grande

quantidade de moléculas orgânicas. Tais transformações biológicas que

envolvem enzimas como catalisadores, freqüentemente provocam alterações

significativas na estrutura e propriedades toxicológicas destas substâncias.

Provavelmente, todos os produtos naturais, independentes da sua

complexidade, podem ser degradados por uma ou outra espécie microbiana

em um determinado ambiente.

No caso de compostos orgânicos (hidrocarbonetos), a biodegradação

leva com freqüência, embora não necessariamente, à conversão de C, N, P, S

e outros elementos presentes no composto original a produtos inorgânicos. Tal

conversão de um substrato orgânico a produtos inorgânicos é conhecida como

mineralização.

A biodegradação consiste no processo de alteração do petróleo pela

ação de microrganismos, tanto em superfície quanto em subsuperfície. Óleos

biodegradados são comumente encontrados acumulados em reservatórios de

subsuperfície com temperaturas menores que 80ºC e com baixa salinidade

(<100 ppm). Nessas condições, tanto microrganismos aeróbios quanto

anaeróbios podem degradar petróleo seguindo caminhos bioquímicos

diferentes (WIDDEL & RABUS, 2001; PRINCE, 2002), dependendo da

disponibilidade de aceptores de elétrons orgânicos e inorgânicos (HEAD,

JONES & LARTER, 2003; LARTER et al., 2003; HOLBA et al., 2004).

A relativa preferência por hidrocarbonetos saturados e aromáticos pelos

microrganismos tem sido vastamente documentada (VOLKMAN et al., 1983;

CONNAN, 1984; ROWLAND et al., 1986; HUNT, 1996; WENGER, DAVIS &

ISAKSEN, 2002; PETERS, WALTERS & MOLDOWAN, 2004). Em geral, os

alcanos de cadeia linear são metabolizados preferencialmente, seguidos pelos

alcanos ramificados, saturados monocíclicos e hidrocarbonetos

monoaromáticos, naftênicos policíclicos e hidrocarbonetos aromáticos

polinucleares, e finalmente compostos heteroatômicos (TISSOT & WELTE,

1984; WENGER, DAVIS & ISAKSEN, 2002). São observadas diferenças na

susceptibilidade de hidrocarbonetos ao ataque microbiano dentro de séries

homólogas e entre formas isoméricas desses compostos (PETERS et al., 1996;

TOWNSEND, PRINCE & SUFLITA, 2004).

Compostos contendo heteroátomos geralmente são mais resistentes ao

metabolismo microbiano do que hidrocarbonetos. Alguns compostos, tais como

vanadilporfirinas, aparecem remanescentes mesmo dentro dos óleos mais

severamente biodegradados (SUNDARARAMAN & HWANG, 1993). Outros

compostos NSO, tais como carbazóis (HUANG et al., 2003), quinolinas

(BENEDIK et al., 1998), e geomacromoléculas maiores (JENISCH-ANTON,

ADAM & ALBRECHT, 2000; PIÑEDA-FLORES & BOLL-ARGÜELO, 2004) são

biomineralizados, variando o grau de biodegradação em laboratório ou em

sistemas naturais.

1.5. MICRORGANISMOS DEGRADADORES DE HIDROCARBONETOS

Muitos microrganismos são eficientes na degradação de hidrocarbonetos

e possuem uma versatilidade metabólica que permite a utilização do petróleo

como única fonte de carbono (BOUWER & ZEHNDER, 1993; VAN HAMME,

SINGH & WARD, 2003). Além disso, uma grande quantidade de

microrganismos com capacidade de degradar diversos compostos, como

benzeno, fenol e naftaleno, já foi isolada e caracterizada (FAISON, 2001;

DICKEL, HAUG & KNACKMUS, 1993). Dentre eles, as bactérias são as mais

estudadas. Este fato se deve à facilidade de cultivo e manipulação genética,

capacidade de metabolizar organoclorados, capacidade de mineralização de

diversos compostos químicos e a utilização dos mesmos como fonte de energia

(BROSSERT & BARTHA, 1984; BOUWER & ZEHNDER, 1993; ATLAS &

CERNIGLIA, 1995).

Já foram realizados estudos sobre as vias de biodegradação de alcanos,

cicloalcanos e hidrocarbonetos aromáticos e policíclicos aromáticos (SMITH,

1990; WATKINSON & MORGAN, 1990; CERNIGLIA, 1992; SUTHERLAND,

1992; KÄMPFER et al., 1993; CERNIGLIA, 1997; JUHASZ & NAIDU, 2000) e

também sobre os mecanismos microbianos de catabolismo anaeróbio de

hidrocarbonetos (FRAZER, COSCHIGANO & YOUNG, 1995; HARWOOD &

GIBSON, 1997; HEIDER et al., 1999; LOVELY, 2000; PHELPS & YOUNG,

2001; WIDDEL & RABUS, 2001). Apesar dos compostos aromáticos e

policíclicos aromáticos serem susceptíveis à degradação por microrganismos,

muitos deles são mais resistentes à biodegradação que os hidrocarbonetos

saturados (VAN BEILEN, WUBBOLTS & WITHOLT, 1994; CHENG, THOMAS

& ROUVIERE, 2002). O estudo dos sistemas enzimáticos envolvidos no

processo de biodegradação de hidrocarbonetos de petróleo é de importância

fundamental para gerar não só conhecimento científico como para a

aplicabilidade do processo.

Embora o potencial dos microrganismos em degradar compostos

orgânicos sob condições favoráveis seja elevado, já foi relatado que cada

microrganismo individualmente pode mineralizar somente um número limitado

de hidrocarbonetos. Ainda não foi descrita nenhuma espécie capaz de

degradar todos os componentes do petróleo e não há relatos de nenhum

“super organismo” degradador de óleo desenvolvido por engenharia genética.

Compostos orgânicos de baixa massa molecular e estrutura molecular simples

são os preferidos pela maioria dos microrganismos (ATLAS & CERNIGLIA,

1995). Compostos com estruturas mais complexas, como HPAs

(Hidrocarbonetos Policíclicos Aromáticos), com mais de cinco anéis de

benzeno, são mais resistentes à ação microbiana (CERNIGLIA et al., 1985;

CERNIGLIA, WHITE & HEFLICH, 1985; BROSSERT & BARTHA, 1986). Dessa

forma, a biodegradação eficaz do petróleo requer uma mistura de populações,

com uma ampla gama de capacidade enzimática, composta de diversos

gêneros, cada qual capaz de metabolizar seus respectivos compostos (KORDA

et al., 1997; GHAZALI et al., 2004). Os principais gêneros de bactérias

envolvidos na degradação de hidrocarbonetos são: Achromobacter,

Acinetobacter, Alcanivorax, Alcaligenes, Bacillus, Brevibacterium, Burkholderia,

Corynebacterium, Geobacillus, Gordonia, Mycobacterium, Nocardia,

Pseudomonas, Rhodococcus, Sphingomonas, Streptomyces e

Thermoleophilum (OLLIVIER & MAGOT, 2005). As bactérias formadoras de

esporos podem resistir melhor à agressão causada pelo óleo, tendo assim um

papel importante na transformação de hidrocarbonetos, já que os esporos são

estruturas capazes de resistir ao calor, dessecação, radiação UV e produtos

químicos tóxicos (DRIKS, 1999).

1.6. BIODEGRADAÇÃO MICROBIANA EM RESERVATÓRIOS

PETROLÍFEROS

A existência de microrganismos autóctones em reservatórios de petróleo

tem sido largamente citada na literatura (MAGOT, OLLIVIER & PATEL, 2000;

HEAD, JONES & LARTER, 2003; LARTER et al., 2003). Entretanto, coletar

amostras representativas de ambientes de subsuperfície como campos de

extração de óleo é a primeira e provavelmente a maior dificuldade para os

microbiologistas.

Nos reservatórios de óleo, as bactérias autóctones supostamente vivem

aderidas às superfícies do reservatório formando biofilmes. A melhor forma de

estudar as populações microbianas destes ambientes é coletar amostras dos

biofilmes e transportar de forma adequada para o laboratório (GRIFFIN et al.,

1997).

A temperatura dos reservatórios aumenta aproximadamente 3ºC a cada

100 m de profundidade. Consequentemente, temperaturas entre 130 e 150ºC

ou maiores podem ser encontradas nestes ambientes, as quais podem ser

consideradas temperaturas limites para o desenvolvimento da vida por causar

instabilidade térmica nas moléculas biológicas (STETTER, HOFFMANN, &

HUBER, 1993). Os microrganismos mais termofílicos recentemente descritos

são capazes de crescer a temperatura de 121ºC e podem sobreviver por 2h a

130ºC (KASHEFI & LOVLEY, 2003). De acordo com estas observações,

microrganismos autóctones podem ser encontrados nos reservatórios que

possuem até 4000 m com temperatura aproximada de 120 a 130ºC. Apesar

disso, dados sugerem que a temperatura limite para todas as formas de vida

nos reservatórios fica longe desses valores, sendo citados na literatura valores

que se encontram entre 80 e 90ºC para ser observada a biodegradação in situ

do óleo. Entretanto, a maior parte da biodegradação do petróleo observada

quase nunca excede os 82ºC, sendo mais freqüentemente observada a

temperaturas inferiores a esse valor. A degradação ocorre então em

micronichos onde a temperatura está dentro da faixa de crescimento dos

microrganismos mesofílicos (ROADIFER, 1987; PHILIPPI, 1997; HEAD,

JONES & LARTER, 2003).

A degradação microbiana durante os processos de produção de petróleo

causa um grande impacto nas propriedades físicas, no volume e na

composição do petróleo. Quando sofrem biodegradação, os óleos se tornam

mais viscosos, ricos em compostos de enxofre, resinas, asfaltenos e metais.

Além disso, os valores de acidez são aumentados com a degradação.

É estimado que o taxa de degradação nos reservatórios seja de

aproximadamente 10

-4

kg/m

/ano. Entretanto, alguns modelos de degradação

sugerem uma perda de até 50% no volume do óleo. Muitas classes de

compostos são degradadas simultaneamente, e, como esses processos

ocorrem muito comumente nos reservatórios, as perdas reais nos processos de

produção são muito grandes (HEAD, JONES & LARTER, 2003).

1.7. ESTUDO DA DIVERSIDADE MICROBIANA

Em função do grande avanço tecnológico nas últimas décadas, foi

possível verificar que o conhecimento acerca da biodiversidade existente no

planeta é praticamente insignificante. Em relação aos microrganismos

existentes, esta falta de conhecimento se deve em grande parte ao fato de que

os microrganismos precisam ser cultivados para serem caracterizados. Sabe-

se que apenas uma pequena fração (1 a 10%) dos microrganismos presentes

no meio ambiente pode ser cultivada utilizando as técnicas convencionais de

cultivo em microbiologia já descritas. Estas técnicas clássicas demandam um

tempo relativamente grande para análise, havendo a possibilidade também de,

após várias gerações, os microrganismos apresentarem alterações fisiológicas

e até genéticas significativas (ROSADO et al., 1997).

O rápido avanço de métodos de microbiologia molecular possibilitou a

introdução do conceito de diversidade em ecologia microbiana para a análise

de fenômenos como sucessão e resposta a distúrbios (MACNAUGHTON et al.,

1999). Com o desenvolvimento das técnicas de biologia molecular, associadas

ao conhecimento em bioinformática, tornou-se possível a caracterização de

comunidades microbianas mistas em determinados ecossistemas, revelando os

grupos atuantes, muitos destes até então desconhecidos. Essa revolução do

conhecimento possibilitou a criação de um novo campo na Microbiologia

Ambiental denominado Ecologia Microbiana Molecular (ROSADO et al., 1997).

Apesar da utilização destas novas técnicas para o estudo da diversidade

microbiana, as técnicas tradicionais, que envolvem enriquecimento e cultivo,

ainda são fundamentais para a obtenção de um conhecimento mais detalhado

a respeito do tipo de metabolismo e da caracterização fenotípica, podendo

desta forma chegar o mais próximo possível da caracterização do ambiente

(MUYZER & SMALLA, 1999).

Analisar a diversidade de comunidades microbianas, em termos de

riqueza e estrutura, é uma forma de se compreender como estas comunidades

reagem no seu ambiente à presença de contaminantes exógenos. De uma

maneira mais ampla, é um meio possível de avaliar a questão da modulação

das comunidades microbianas por contaminantes e fatores ambientais. Muitas

dificuldades são encontradas durante as tentativas de caracterizar as

comunidades microbianas naturais em ambientes com hidrocarbonetos do

petróleo. Apesar das dificuldades, muitas ferramentas estão sendo

desenvolvidas na tentativa de melhorar os estudos que visam quantificar os

microrganismos e verificar a sua distribuição no ambiente natural, na

esperança de associar essas comunidades com funções do ecossistema (VAN

HAMME, SINGH & WARD, 2003).

Amostras ambientais podem ser usadas para o isolamento de estirpes

microbianas ou para o isolamento de ácidos nucléicos para posterior análise.

Dessa forma, os métodos para analisar a diversidade microbiana e a função da

comunidade no ambiente podem ser divididos em métodos dependentes de

cultivo e independentes de cultivo, sendo que ambos devem incluir técnicas de

caracterização genética (VAN HAMME, SINGH & WARD, 2003).

1.8. MÉTODOS DEPENDENTES DE CULTIVO NO ESTUDO DA

DIVERSIDADE MICROBIANA

Tradicionalmente, a diversidade pode ser acessada pelo uso de métodos

dependentes de cultivo, baseados nas características morfológicas e

fisiológicas dos microrganismos. Isto inclui o inóculo em meios de cultura,

contagem dos microrganismos viáveis, a seleção e o isolamento de diferentes

morfotipos coloniais, a caracterização e a identificação dos morfotipos isolados,

coloração de Gram, microscopia e análise bioquímica (WATANABE et al.,

1998; VAN HAMME, SINGH & WARD, 2003; KIRK et al., 2004).

Com relação aos isolados de ambientes contaminados, é importante

também determinar a capacidade de degradação do poluente, suas frações e

compostos derivados (VAN HAMME, SINGH & WARD, 2003; DAS &

MUKHERJEE, 2007). Estas análises devem ser complementadas com técnicas

de caracterização genética (VAN HAMME, SINGH & WARD, 2003).

Os métodos de análise microbiológica clássica podem ser rápidos e de

baixo custo e podem fornecer informações sobre o componente heterotrófico

ativo da população (BALBA, AL-AWANDHI & AL-DAHER, 1998; KIRK et al.,

2004). Além disso, existem sistemas comercialmente disponíveis para avaliar o

potencial fisiológico microbiano através de padrões de assimilação e

fermentação de diversas fontes de carbono como Biolog (Hayward, CA, USA,

www.biolog.com) e API (bioMérieux, France) que geram um perfil fisiológico ou

metabólico da comunidade bacteriana ou fúngica estudada (CLASSEN et al.,

2003; KIRK et al., 2004). Estas metodologias são relativamente fáceis de usar,

são reprodutíveis, produzem uma grande quantidade de dados refletindo as

características metabólicas das comunidades e já foram aplicadas com

bastante sucesso em diversos estudos de ecologia microbiana (GARLAND &

MILLS, 1991; ZAK et al., 1994; CAMPBELL, GRAYSTON & HIRST, 1997;

BECKER & STOTTMEISTER, 1998; CHOI & DOBBS, 1999; CLASSEN et al.,

2003).

As técnicas microbiológicas tradicionais têm sido usadas com grande

sucesso na caracterização de espécies microbianas isoladas de ambientes

contaminados. No entanto, uma vez que os procariotos são bastante diversos,

mas esta diversidade nem sempre se reflete na morfologia (apresentando

diferenças morfológicas relativamente pequenas que, por vezes, dificultam a

distinção até mesmo com a utilização de microscópio), a utilização de métodos

clássicos dependentes de cultivo e de comparação morfológica acabam por

refletir uma parcela muito pequena da comunidade analisada. Além disso, as

culturas do enriquecimento acabam favorecendo as espécies bacterianas de

crescimento rápido e aquelas capazes de crescer nos meios e nas condições

experimentais utilizadas (GARLAND & MILLS, 1991; GARLAND, 1996; BALBA,

AL-AWANDHI & AL-DAHER, 1998; TABACCHIONI et al., 2000; YAO et al.,

2000; TORSVIK & OVREAS, 2002; KASSEN & RAINEY, 2004; KIRK et al.,

2004). Dessa forma, as técnicas de microbiologia clássica invariavelmente

subestimam a diversidade estrutural e funcional como resultado da dificuldade

existente em cultivar a maioria dos microrganismos do ambiente. De fato, já foi

observado em muitas amostras ambientais que as bactérias que são as mais

dominantes e abundantes nem sempre são cultiváveis (TORSVIK & OVREAS,

2002).

1.9. MÉTODOS INDEPENDENTES DE CULTIVO NO ESTUDO DA

DIVERSIDADE MICROBIANA

A introdução de técnicas moleculares na análise da estrutura da

comunidade bacteriana tem aumentado nossa capacidade de entender a

diversidade microbiana de forma mais clara, levando ao aumento significativo

do número de grupos filogenéticos no domínio Bacteria (AMANN & LUDWIG,

2000).

Atualmente, estas técnicas vêm sendo utilizadas para caracterizar os

ácidos nucléicos dos microrganismos contidos na comunidade microbiana de

amostras ambientais, o que tem ajudado na descoberta de um grande número

de microrganismos que não podem ser cultivados em laboratório, mas que

desempenham um papel ecológico significativo em seus respectivos

ambientes. O principal benefício dessas análises moleculares é a possibilidade

de estudar comunidades microbianas sem a necessidade do cultivo, pois as

análises de microbiologia clássica são indiretas e provocam mudanças na

estrutura e na atividade metabólica da comunidade microbiana. Os métodos

moleculares diretos, no entanto, preservam o ‘status’ metabólico in situ e a

composição da comunidade microbiana, porque as amostras são congeladas

imediatamente após a coleta. Também, a extração direta dos ácidos nucléicos

de amostras ambientais conta com uma proporção muito maior de

microrganismos que não são cultiváveis em laboratório, mas podem estar

relacionadas à atividade biodegradadora de interesse (BROCKMAN, 1995).

Estas vantagens, associadas ao longo período necessário para os métodos de

cultivo, têm resultado na popularidade de abordagens moleculares para o

estudo das populações bacterianas.

A análise das variações que ocorrem na população de um local

contaminado por métodos moleculares, acompanhada por uma mudança na

função de uma comunidade, auxilia na identificação das populações

funcionalmente dominantes (BOND et al., 1995; BORNEMAN & TRIPLETT,

1997). Entretanto, os pesquisadores têm enfatizado que experimentos de

cultura pura são indispensáveis para a análise detalhada das funções de cada

população e que o isolamento das populações funcionalmente dominantes em

uma comunidade microbiana é muito importante (WATANABE et al., 1998).

Existe uma vasta gama de métodos disponíveis para o estudo da

diversidade estrutural e funcional da comunidade microbiana em ambientes

contaminados. Cada metodologia tem suas limitações e fornece apenas

informação parcial de um aspecto da diversidade microbiana. As técnicas

moleculares que têm sido usadas para superar as limitações das metodologias

clássicas também apresentam suas próprias limitações (KIRK et al., 2004).

Alguns estudos de análise da diversidade microbiana foram realizados

através da utilização de uma combinação de métodos dependentes

(microbiologia clássica) e independentes (moleculares) de cultivo. Foram

utilizadas diversas técnicas para determinar os efeitos de poluentes sobre a

estrutura e/ou função da comunidade microbiana (EL FANTROUSSI et al.,

1999; RÖLING et al., 2000; ELLIS et al., 2003). Uma visão mais completa

dessa diversidade pode ser obtida através do uso de uma variedade de

metodologias, cada uma delas com um enfoque diferente (KIRK et al., 2004).

É válido ressaltar que os métodos microbiológicos clássicos combinados

aos métodos moleculares contribuem para uma melhor avaliação da

comunidade microbiana do local estudado (BROCKMAN, 1995). É necessário

um processo multidisciplinar, incorporando métodos microbiológicos e

moleculares. A combinação de técnicas de microbiologia clássica e de técnicas

moleculares pode fornecer uma visão mais global das transformações que

ocorrem no ambiente, aumentando a compreensão dos mecanismos de

biodegradação. Assim, estimativas da diversidade bacteriana devem incluir

metodologias múltiplas integrando medidas para análise da comunidade total

bem como análises parciais dos subconjuntos estruturais e funcionais (BALBA,

AL-AWANDHI & AL-DAHER, 1998; WATANABE et al., 1998).

2. JUSTIFICATIVA E OBJETIVOS GERAIS

O petróleo é uma fonte de energia essencial que impulsiona a

industrialização e o contínuo crescimento econômico. O petróleo fornece 39%

da energia mundial, e a grande maioria dos meios de transporte é movida por

produtos derivados do refino do óleo cru. Entretanto, processos decorrentes da

atividade microbiana podem afetar a produção e a qualidade do petróleo

produzido. Um exemplo é a degradação microbiana de hidrocarbonetos que

resultam na diminuição da sua densidade, acidez e viscosidade do petróleo.

Essas mudanças nas características do óleo possuem conseqüências

negativas para produção e refino do petróleo, reduzindo a qualidade do produto

assim como seu valor econômico. A atividade microbiana nos reservatórios de

petróleo pode ser ainda intensificada como resultado da ação antropogênica.

Na recuperação secundária do petróleo há a introdução de água para forçar a

saída do óleo do reservatório, juntamente com a água são introduzidos também

microrganismos exógenos, nutrientes e aceptores de elétrons que estimulam o

crescimento microbiano e que podem promover a acidulação do poço. Embora

a comunidade microbiana presente nos fluidos produzidos de poços produtores

de óleo seja de grande importância para as indústrias petrolíferas, esta é ainda

pouco conhecida. Esse fato deve-se principalmente à dificuldade de obtenção

da amostra e às suas características químicas que dificultam a manipulação e

os procedimentos de extração de DNA. Sendo assim, o primeiro objetivo foi

adaptar a metodologia de extração de ácidos nucléicos existente para esse tipo

de amostra. A partir do DNA total extraído dos fluídos petrolíferos produzidos

provenientes de duas plataformas de petróleo, foi determinada a influência do

teor de água (porcentagem de BSW) presente nos fluidos na diversidade da

comunidade bacteriana caracterizada no presente estudo (por métodos

independentes de cultivo). Após a utilização de métodos dependentes de

cultivo, estirpes bacterianas das quatro amostras de fluido foram ainda

estudadas quanto ao potencial de degradação de óleo cru.

3. OBJETIVOS ESPECÍFICOS

 Extrair o DNA total de quatro amostras de fluido produzido provenientes

de duas plataformas de petróleo brasileiras. Em cada uma das

plataformas foi analisado um fluido com alto BSW e outro com baixo

BSW.

 Amplificar por PCR utilizando-se iniciadores universais baseados no

gene que codifica o 16S rRNA para o domínio Bacteria.

 Construir bibliotecas de clones a partir dos fragmentos gerados por

PCR.

 Analisar filogeneticamente as seqüências obtidas dos clones estudados.

 Comparar a comunidade bacteriana presente nos diferentes fluidos

provenientes das duas plataformas.

 Isolar estirpes bacterianas das quatro amostras de fluido.

 Caracterizar as estirpes indígenas do óleo que apresentem potencial de

degradação.

4. METODOLOGIA

4.1. MEIOS DE CULTURA

• BH - Bushnell-Hass (Difco)

Bushnell-Hass 3,27 g

Água destilada (q.s.p.) 1000 ml

pH 7,0 ± 0,2

Obs.: O meio contém por litro: Sulfato de Magnésio – 0,2 g, Cloreto de

Cálcio – 0,02 g, Fosfato de Monopotássio – 1 g, Fosfato de Amônia Dibásico –

1 g, Nitrato de Potássio – 1 g, Cloreto Férrico – 0,05 g.

• LB -“Luria-Bertani Broth” (SCHLEIF & WENSINK,1981)

Triptona 10 g

Extrato de levedura 5 g

NaCl 5 g

Água destilada (q.s.p.) 1000 ml

pH 7,2

• TSB -“Trypticase Soy Broth” (Difco)

“Trypticase Soy Broth” 30 g

Água destilada (q.s.p.) 1000 ml

pH 7,3

• MB – “Marine Broth” (Difco)

“Marine Broth” 37,4 g

Água destilada (q.s.p.) 1000 ml

pH 7,6

Obs.: Sempre que necessário, os meios de cultura foram acrescidos de

ágar na concentração de 1,2%.

4.2. SOLUÇÕES

Solução corante de corrida para eletroforese de DNA em gel de agarose

Glicerol 50 ml

EDTA, pH 7,5 20 mM

Azul de bromofenol 0,05 g

Xileno cianol 0,05 g

Água destilada q.s.p. 100 ml

Solução de RNase A (Sigma Chemical Co.)

A solução de RNase A foi preparada em água bidestilada em uma

concentração final de 10mg/ml e tratada a 80ºC por 10 min. A solução foi

estocada a -20ºC.

Solução de Pronase E (Sigma Chemical Co.)

A solução de Pronase E foi preparada em água bidestilada em uma

concentração final de 10mg/ml e tratada a 37ºC por 30 min. A solução foi

estocada a -20ºC.

4.3 . TAMPÕES

Tampão TEB

Tris 89 mM

EDTA 2,5 mM

89 mM

Água destilada q.s.p. 1000 ml

pH 7,8

Tampão TE

Tris 10 mM

EDTA 1 mM

Água destilada q.s.p. 1000 ml

pH 7,8

Tampão de lise

Sacarose 25% (p/v)

Tris 50 mM

EDTA 1 mM

pH 7,8

Tampão de Winogradsky

HPO

0,25 g

MgSO

.7H

O 0,25 g

NaCl 0,125 g

(SO

)

O 2,5 g

MnSO

O 2,5 g

Após o preparo os tampões foram autoclavados a 121ºC por 20 min e

estocados a temperatura ambiente.

4.4. CARACTERIZAÇÃO GENOTÍPICA

4.4.1. AMOSTRAGEM

Um total de quatro amostras de fluido produzido foi coletado de duas

plataformas da Bacia de Campos (P-43 e P-48) por membros do CENPES,

PETROBRAS. Foi obtida, de cada plataforma, uma amostra contendo alto

BSW e uma contendo baixo BSW (P-43 - BR-36 BSW de 1% e BR-25 BSW de

40%; P-48 - CRT-12 BSW de 60% e CRT-37 BSW de 5%). As amostras são

citadas no texto como P-43 BR-36 = P43 36, P-43 BR-25 = P43 25, P-48 CRT-

12 = P48A e P-48 CRT-37 = P48B.

4.4.2. EXTRAÇÃO DE DNA GENÔMICO

Para a recuperação das células bacterianas presentes em cada uma das

amostras, partes iguais de tampão de Winogradsky e fluido extraído dos

reservatórios foram introduzidos em tubos Falcon de 50 ml (10 ml de tampão e

10 ml de fluido). Os tubos foram colocados em banho-maria a 80ºC por 10 min,

o tampão foi retirado da fase inferior do tubo com o auxílio de uma pipeta e foi

reservado. Este procedimento foi realizado por 5 vezes, obtendo-se um total de

50 ml de tampão recuperado das lavagens do óleo, para cada amostra.

A extração de DNA genômico foi realizada utilizando-se fenol, segundo

descrito por SELDIN & DUBNAU (1985). Os tampões recuperados foram

submetidos à centrifugação (centrífuga Sorvall RC5B com rotor SS-34) a

temperatura ambiente a 8.000 rpm durante 15 min. O precipitado foi lavado

com TE e recentrifugado nas mesmas condições. O precipitado foi

ressuspenso em 2 ml de tampão de lise e acrescido de 2 mg de lisozima,

permanecendo por 30 min a 37ºC. Foram adicionados então 200 µl de SDS

(dodecil sulfato de sódio) a 10% e cada preparação foi incubada por mais 10

min a 37ºC. Foram adicionados 10 µl de solução de RNase A e incubado por

10 min a 37ºC. Em seguida foram adicionados 220 µl de solução de Pronase E

e, após 2 h a 37ºC, o DNA foi precipitado com 250 µl de acetato de sódio (3M,

pH 7,0) e 6 ml de etanol absoluto por uma noite a -20ºC.

O DNA foi recuperado por centrifugação a 4ºC por 15 min a 10.000 rpm

(centrifuga Sorvall RC5B com rotor SS-34) e ressuspenso em 1,5 ml de TE.

Após uma noite a 4ºC, foi tratado com 500 µl de fenol e centrifugado (centrífuga

Eppendorf 5410) a temperatura ambiente, 10.000 rpm por 5 min. A fase aquosa

foi tratada duas vezes com o mesmo volume de clorofórmio-álcool isoamílico

(24:1) e centrifugada nas mesmas condições. O DNA foi então precipitado com

1/10 do volume de acetato de sódio (3M, pH 7,0) e 2 volumes de etanol

absoluto gelado por uma noite a -20ºC. As preparações foram centrifugadas a

4ºC por 15 min a 10.000 rpm (centrífuga Eppendorf 5410) e o precipitado foi

lavado com etanol a 70 %, ressuspenso em 300 µl de TE e mantido a 4 ºC.

4.4.3. PCR BASEADO NO GENE QUE CODIFICA O 16S rRNA

As reações de amplificação do gene que codifica o 16S rRNA da

comunidade bacteriana presente nos fluidos foram realizadas com os

iniciadores U968 F e L1401 R (Tabela 1), como descrito por Heuer & Smalla

(1997).

Iniciadores Seqüências

U968 F

5’ AACGCGAAGAACCTTAC 3’

L1401 R 5’ CGGTGTGTACAAGACCC 3’

Cada reação foi realizada em tubo contendo 5 µl do tampão de PCR 10X

da Promega



, 2,5 mM MgCl

, 2 mM de cada um dos quatro dNTPs, 20 pmol de

cada iniciador, 20 µg de soro albumina bovina, 2,5 U de Taq DNA polimerase, 1

µl (50 a 100 ng) de DNA e água bidestilada estéril q.s.p. 50 µl. O ciclo aplicado

foi: 1x (2 min 94ºC); 30x (1 min 94ºC; 1 min 55ºC; 2 min 72ºC); 1x (10 min

72ºC); 4ºC.

4.4.4. ELETROFORESE EM GEL DE AGAROSE

Os fragmentos obtidos nas reações de PCR foram submetidos à

eletroforese em gel de agarose a 1,4% preparado em tampão TEB, por 2 h a

80 V. Dez microlitros do produto de PCR foram misturados com o corante para

eletroforese na proporção do volume 3:1 (DNA:corante), e em seguida

aplicados no gel. Foram incluídos nos géis padrões de tamanho de DNA linear

(1 Kb DNA Ladder, Promega), bem como controles negativos (reação sem

DNA). Após a eletroforese, os géis foram corados por 15 minutos com brometo

de etídio (EtBr) na concentração de 2 µg/ml, a DNA foi observado em um

transiluminador ultravioleta, em um comprimento de onda de 300 nm. A

especificidade da reação foi sugerida com o aparecimento de apenas uma

banda de tamanho esperado para cada um dos pares de iniciadores utilizados.

Tabela 1

–

Iniciadores que foram utilizados nas reações de amplificação por PCR

do gene que codifica o 16S rRNA da comunidade bacteriana total

4.4.5. CLONAGEM E SEQÜENCIAMENTO

Os produtos de PCR foram clonados utilizando-se o kit de clonagem

“Clone Jet

PCR Cloning Kit” (Fermentas). Para tal, foram colocados em um

tubo Eppendorf de 500 µl (para a reação de “blunting”), 10 µl de tampão da

reação, 2 µl do produto de PCR, 5 µl de água (isenta de nucleases) e 1 µl “DNA

blunting enzyme”. Após agitação em vortex por 5 seg, os tubos foram

incubados a 70

C por 5 min e em seguida resfriados brevemente em gelo. Para

a reação de ligação, foram adicionados ao tubo da reação 1 µl do pJET 1.2 –

“blunt cloning vector” (50 ng/µl) e 1 µl da T

DNA ligase (5 M/µl). Após agitação

em vortex por 5 seg, os tubos foram incubados a 22

C por 5 min. Foram

acrescentados 2,5 µl da reação de ligação a um tubo Eppendorf de 500 µl

contendo 70 µl de células competentes de Escherichia coli JM109. Em seguida,

essas células foram transformadas através de eletroporação (choque de 1,8

kV, 200 Ω, 25 µF), utilizando uma cubeta com espaçamento de 0,1 cm. Após a

transformação, as células foram mantidas em meio LB por 1,5 h a 37ºC sob

agitação. Uma quantidade de 100 µl de células transformadas foi semeada em

placas contendo meio LB com ampicilina (100 µg/ml). As colônias capazes de

crescer nesse meio foram consideradas como possíveis portadoras do

plasmídio contendo o fragmento oriundo das reações de PCR.

Para a confirmação da presença do inserto nos clones, foi feita uma

reação de PCR diretamente da colônia, utilizando-se os iniciadores pJETf (5’-

GCCTGAACACCATATCCATCC-3’) e pJETr (5’-

GCAGCTGAGAATATTGTAGGAGATC-3’). Após a confirmação, os DNAs

plasmidiais dos transformantes foram extraídos com auxílio do kit comercial

“Wizard Plus SV miniprep DNA purification system” (Promega) e então

submetidos à reação de PCR para seqüenciamento, em um seqüenciador

automático ABI Prism 3100. As seqüências obtidas foram analisadas com o

intuito de verificar a sua qualidade e para retirada de seqüências

correspondentes ao vetor.

4.5. ANÁLISES DAS SEQUÊNCIAS

As seqüências selecionadas para este estudo foram então comparadas

com as presentes no banco de dados GenBank utilizando-se a ferramenta

Blastn (Basic Local Alignment Search Tool;

http://blast.ncbi.nlm.nih.gov/Blast.cgi) e com o banco de dados do RDP

(Ribossomal Database Project;

http://rdp.cme.msu.edu/) no dia 11/01/2010.

As seqüências foram alinhadas utilizando o programa Bioedit (HALL,

1999) para contrução de árvores filogenéticas utilizando o programa Mega 3.1

(KUMAR, DUDLEY & TAMURA, 2008)

O programa webLIBSHUFF (http://libshuff.mib.uga.edu) foi utilizado nas

análises estatísticas para comparação (duas a duas) das seqüências

pertencentes às quatro bibliotecas obtidas (SINGLETON et al., 2001). Esse

programa gera uma matriz baseada no número de seqüências de cada

biblioteca e calcula a cobertura homóloga e heteróloga entre as bibliotecas. A

estatística Cramér von-Mises foi utilizada para verificar se as bibliotecas são

significativamente diferentes e estas foram consideradas significativamente

diferentes quando o valor de p gerado foi menor que 0,025.

Foram utilizados também para análises de estimativa de diversidade os

índices de Shannon (SHANNON & WEAVER, 1949), de dominância (TATON et

al., 2003) e o de cobertura (CHELIUS & TRIPLETT, 2001).

4.6. ISOLAMENTO DAS ESTIRPES

Os fluidos extraídos dos reservatórios foram lavados em tubos Falcon de

50 ml. Partes iguais de tampão de Winogradsky e fluido foram colocados nos

tubos (10ml de tampão e 10ml de fluido), mantidos em banho-maria a 40ºC por

10 min, quando o tampão foi retirado e reservado. Este procedimento foi

realizado por 5 vezes obtendo-se um total de 50 ml de tampão para cada

amostra. Os tampões foram centrifugados (centrífuga Sorvall RC5B com rotor

SS-34) a temperatura ambiente a 8000 rpm durante 15 min.

O precipitado foi ressuspendido em 1 ml de água destilada estéril. Após

este procedimento foram plaqueados 100 µl de amostra, em duplicata, nos

meios TSB, LB, MB. As placas contendo inóculo foram mantidas em estufa a

32ºC por até 7 dias para a observação de crescimento de colônias. As estirpes

isoladas foram selecionadas pela diferença na morfologia colonial (forma,

tamanho, coloração e textura da colônia) e foram coradas através da coloração

de Gram. Os diferentes isolados foram mantidos em tubos de ágar inclinado

contendo o mesmo meio do isolamento, à temperatura ambiente.

4.7. TESTE DE DEGRADAÇÃO DE ÓLEO

Este teste foi realizado em placas de 24 poços. Os isolados foram

crescidos em 3 ml do mesmo meio de isolamento a 32ºC por 48 h. A cultura foi

centrifugada (centrífuga Sorvall RC5B com rotor SS-34) a temperatura

ambiente a 8000 rpm durante 15 min e lavada duas vezes em solução salina

0,9%. Cada cavidade da placa contendo 1,8 ml de meio Bushnell Haas foi

inoculada com 200 µl deste pré-inóculo e uma gota de óleo cru. As placas

foram incubadas por 15 dias a 32ºC. A primeira cavidade não foi inoculada,

sendo esta usada como controle negativo. Qualquer alteração na superfície do

óleo diferente do controle negativo foi considerada como um teste positivo.

5. RESULTADOS

5.1. EXTRAÇÃO DE DNA

O método utilizado para a extração de DNA total a partir das amostras

de fluidos petrolíferos (descrito no item 4.4.2) foi eficaz para as quatro amostras

estudadas (P43 25, P43 36, P48A e P48B). Esse resultado demonstra que as

lavagens com tampão de Winogradsky, que precederam a extração por lise

química, foram eficientes na recuperação de células microbianas dos fluidos

petrolíferos tanto de baixo BSW quanto de alto BSW. Além disso, o DNA

extraído apresentava pureza suficiente para ser utilizado em técnicas

moleculares.

5.2. AMPLIFICAÇÃO POR PCR DO DNA TOTAL OBTIDO A PARTIR

DAS AMOSTRAS DE FLUIDOS PETROLÍFEROS

O DNA obtido a partir das quatro amostras de fluidos petrolíferos foi

utilizado como molde para amplificação por PCR utilizando-se iniciadores

específicos para o gene que codifica o 16S rRNA de Bacteria (apresentado na

Tabela 1). Foram obtidos produtos de PCR de tamanho esperado (433 pb)

utilizando os iniciadores específicos para o gene 16S rRNA do domínio

Bacteria.

5.3. CONSTRUÇÃO DE BIBLIOTECAS DE CLONES

Os produtos de PCR obtidos utilizando-se os iniciadores U968 e L1401 a

partir do DNA das quatro amostras de fluidos foram clonados conforme descrito

no item 4.4.5. Ao todo foram obtidos 154 clones, aproximadamente 30-40 por

amostra de fluido produzido (Tabela 2). Após a comparação através da

ferramenta Blastn de todas as seqüências obtidas com outras seqüências

presentes no banco de dados GenBank, foi observado que a distribuição

filogenética dos clones com base no gene que codifica o 16S rRNA foi bastante

homogênea entre as quatros amostras analisadas e que a grande maioria das

seqüências obtidas pertencia à classe Gammaproteobacteria (Figura 2).

Tabela 2 – Número total de clones pertencentes a cada um dos gêneros

encontrados nas bibliotecas e índices de diversidade

Em todas as bibliotecas (Tabela 2 e Figura 2) foram encontrados

majoritariamente clones afiliados a Pseudomonas sp. Uma alta porcentagem

de identidade (99% ou mais) foi observada entre a maioria das seqüências

obtidas no presente estudo e seqüências depositadas no GenBank (Anexo I).

Entretanto também foram observadas seqüências que apresentaram menos de

97% de identidade com as seqüências dos bancos de dados em todas as

amostras analisadas (Anexo I).

As diferentes seqüências das quatro bibliotecas foram utilizadas para a

determinação dos índices de Shannon (SHANNON & WEAVER, 1949), de

Dominância (TATON et al., 2003) e de cobertura (CHELIUS & TRIPLETT,

2001), conforme demonstrado na Tabela 2. Nessa tabela também se

encontram compilados o número total de clones e os diferentes gêneros

encontrados em cada uma das bibliotecas construídas. Com a determinação do

índice de cobertura, foi demonstrado que o número de clones avaliado por

Classificação (nível de

gênero) P48 B P48 A P43 36 P43 25

Total

clones

Pseudomonas

28 17 30 36 111

Acinetobacter

2 0 2 1 5

Burkholderia

1 0 0 0 1

Stenotrophomonas

0 0 1 0 1

Janthinobacterium

5 1 2 3 11

Marinobacter

1 1 0 0 2

Cedecea

1 0 1 0 2

Comamonas

0 0 1 0 1

Acidovorax

0 0 1 1 2

Bacillus

0 1 1 0 2

Dendrosporobacter

0 0 0 1 1

Tistrella

0 13 0 0 13

Ochrobactrum

0 1 0 0 1

Simplicispira

0 1 0 0 1

No. clones (n)

38 35 39 42 154

Índice de Cobertura

92% 86% 87% 93%

Índice de Dominância 1,357143

2,058824

1,3 1,166667

Índice de Shannon 0,934028

1,22652

0,976164

0,58761

biblioteca foi suficiente para representar de 86 a 93% da diversidade das

amostras. Com o cálculo dos índices de Shannon, foi observado que as

seqüências provenientes das amostras da plataforma P48 são mais diversas

que as amostras da plataforma P43. O índice de dominância corrobora os

dados do índice de Shannon, já que os maiores valores foram observados nas

amostras da plataforma P48.

Gamaproteobacteria

Betaproteobacteria

Alphaproteobacteria

Firmicutes

mon

Acinetobacter

Ste

notrop

mon

Marinob

cte

Cede

Burk

hol

ria

nthin

rium

Com

Aci

vora

pli

isp

hroba

strell

acillus

Dendrosporobacter

P48 B

P48 A

P43 36

P43 25

Figura 2 – Número de clones pertencentes

a cada gênero bacteriano por

amostra de Fluido e suas respectivas classes.

Na amostra P48A (BSW 60%) foram obtidos 35 clones. Desses 35, 17

seqüências alinharam com seqüências de Pseudomonas e outras 13 com o

gênero Tistrella. Os 5 clones restantes puderam ser afiliados a

Betaproteobacteria (2 clones), Alphaproteobacteria (2 clones e a

Gammaproteobacteria (1 clone identificado como pertencente ao gênero

Marinobacter ) (Tabela 2 e Figura 2).

Na amostra P48B (BSW 5%), foram obtidos 38 clones. Desses, 28

clones foram afiliados com Pseudomonas (Gammaproteobacteria). Outros 4

clones foram também relacionados à Gammaproteobacteria, 6 clones à

Betaproteobacteria (Tabela 2 e Figura 2).

Seguem abaixo as árvores filogenéticas dos clones das amostras P48A

e P48B (Figuras 3 e 4). As setas indicam os clones que apresentaram menos

de 97% de identidade com as seqüências dos bancos de dados (P48A 6, P48A

32, P48A 35 e P48B 11, P48B 20, P48B 31, P48B 39). Esses dados podem ser

observados também no Anexo I.

P48A-1

P48A-29

P48A-3

P48A-12

P48A-11

P48A-27

P48A-33

P48A-10

P48A-15

P48A-20

P48A-19

P48A-18

P48A-34

P48A-13

P48A-16

P48A-31

Pseudomonas fragi (T); ATCC4973; AF094733

P48A-30

Marinobacter hydrocarbonoclasticus (T); VT8; AJ000726

P48A-8

P48A-32

Simplicispira metamorpha (T); LMG4339; AF078757

P48A-26

P48A-35

Tistrella mobilis (T); AB071665

P48A-5

P48A-7

P48A-23

P48A-17

P48A-22

P48A-2

P48A-6

P48A-9

P48A-24

P48A-21

P48A-28

P48A-4

P48A-14

P48A-25

0.02

Figura 3

– Filogenia dos clones da amostra P48A (BSW 60%). O método

utilizado foi o neighbour-joining e p-distance baseado em um alinhamento de 400

pares de bases do gene que codifica o 16SrRNA com um boostrap de 1000

replicações. Os clones indicados com setas alinharam com menos de 97% de

similaridade com as seqüências no RDP e no NCBI. As estirpes-tipo que

obtiveram maior similaridade com os clones estão incluídas na árvore.

Figura 4

– Filogenia dos clones da amostra P48B (BSW 5%). O método utilizado

foi o neighbour-joining e p-distance baseado em um alinhamento de 400

pares de

bases do gene que codifica o 16SrRNA com um boostrap de 1000 replicações.

Os clones indicados com setas alinharam com menos de 97% de similaridade

com as seqüências no RDP e no NCBI. As estirpes-tipo que obtiveram maior

similaridade com os clones estão incluídas na árvore.

P48B-4

P48B-5

P48B-1

P48B-28

P48B-33

P48B-38

P48B-9

P48B-30

P48B-32

P48B-2

P48B-3

P48B-12

P48B-16

P48B-35

P48B-22

P48B-6

Pseudomonas poae (T); typestrain: DSM14936=LMG21465; P527/13; AJ492829

P48B-17

P48B-27

Pseudomonas corrugata (T); ATCC29736; D84012

P48B-13

P48B-29

P48B-19

Pseudomonas lundensis (T); ATCC 49968T; AB021395

P48B-39

P48B-8

P48B-23

P48B-36

P48B-31

P48B-10

Janthinobacterium lividum (T); DSM 1522T; Y08846

P48B-25

P48B-26

P48B-14

P48B-21

Acinetobacter ursingii (T); LUH3792Type; AJ275038

P48B-34

P48B-15

P48B-11

P48B-18

P48B-20

Pseudomonas hibiscicola (T); ATCC19867T; AB021405

P48B-37

P48B-7

Pseudomonas geniculata (T); ATCC19374T; AB021404

100

0.01

Na amostra P43 25 (BSW 40%), foram obtidos 42 clones. Do total, 36

clones foram identificados como pertencentes ao gênero Pseudomonas

(Gammaproteobacteria), 4 clones pertencentes a Betaproteobacteria, 1 clone a

outro gêneros da classe Gammaproteobacteria e um único clone que foi

afiliado à Dendrosporobacter (Firmicutes) (Tabela 2 e Figura 2).

Na amostra P43 36 (BSW 1%) foram obtidos ao todo 39 clones. Desses,

30 clones foram afiliados com Pseudomonas (Gammaproteobacteria). Outros

4 clones foram também relacionados à Gammaproteobacteria, 4 clones foram

relacionados à Betaproteobacteria, 1 clone relacionado ao gênero Bacillus

(Firmicutes) (Tabela 2 e Figura 2).

Seguem abaixo as árvores filogenéticas dos clones das amostras P43-

25 e P43-36 (Figuras 5 e 6). As setas indicam os clones que apresentaram

menos de 97% de identidade com as seqüências dos bancos de dados (P43-25

1, P43-25 14, P43-25 17, P43-25 19, P43-25, 21 e P43-36 17, P43-36 19),

esses dados podem ser observados também no Anexo I.

Figura 5

– Filogenia dos clones da amostra P43-25 (BSW 40%). O método

utilizado foi o neighbour-joining e p-distance baseado em um alinhamento de 400

pares de bases do gene que codifica o 16SrRNA com um boostrap de 1000

replicações. Os clones indicados com setas alinharam com menos de 97% de

similaridade com as seqüências no RDP e no NCBI. As estirpes-tipo que

obtiveram maior similaridade com os clones estão incluídas na árvore.

P43-25-22

P43-25-25

P43-25-37

P43-25-2

P43-25-24

P43-25-26

Pseudomonas antarctica (T); type strain: CMS 35; AJ537601

P43-25-16

P43-25-35

P43-25-27

P43-25-13

P43-25-36

P43-25-39

P43-25-12

P43-25-28

P43-25-38

P43-25-15

P43-25-33

P43-25-8

P43-25-6

P43-25-7

P43-25-31

P43-25-18

P43-25-29

P43-25-32

P43-25-9

P43-25-19

P43-25-20

P43-25-30

P43-25-42

P43-25-4

P43-25-10

Pseudomonas rhodesiae (T); CIP104664; AF064459

P43-25-11

P43-25-40

P43-25-17

P43-25-3

Acidovorax temperans (T); CCUG11779; AF078766

P43-25-14

P43-25-34

Janthinobacterium lividum (T); DSM 1522T; Y08846

P43-25-1

P43-25-21

P43-25-5

P43-25-23

Pseudomonas geniculata (T); ATCC19374T; AB021404

P43-25-41

Dendrosporobacter quercicolus (T); M59110

0.01

Figura 6

– Filogenia dos clones da amostra P43-36 (BSW 1%). O método utilizado

foi o neighbour-joining e p-distance baseado em um alinhamento de 400 pares de

bases do gene que codifica o 16SrRNA com um boostrap de 1000 replicações. Os

clones indicados com setas alinharam com menos de 97% de similaridade com as

seqüências no RDP e no NCBI. As estirpes-tipo que obtiveram maior similaridade

com os clones estão incluídas na árvore.

P43-36-11

P43-36-29

P43-36-21

P43-36-34

P43-36-23

P43-36-37

P43-36-1

P43-36-3

P43-36-32

P43-36-35

Pseudomonas trivialis (T); type strain: DSM 14937 = LMG 21464; P 513/19; AJ492831

P43-36-2

P43-36-33

P43-36-16

P43-36-24

P43-36-7

P43-36-27

P43-36-4

P43-36-28

P43-36-8

P43-36-15

P43-36-30

P43-36-13

P43-36-14

P43-36-18

P43-36-22

P43-36-6

P43-36-38

P43-36-25

P43-36-26

P43-36-31

P43-36-19

P43-36-12

Janthinobacterium lividum (T); DSM 1522T; Y08846

P43-36-39

P43-36-20

Comamonas aquatica (T); typestrain: LMG2370; AJ430344

Acidovorax temperans (T); CCUG11779; AF078766

P43-36-9

P43-36-17

Stenotrophomonas maltophilia (T); ATCC13637T; AB008509

P43-36-5

Cedecea davisae (T); DSM4568; AF493976

P43-36-10

P43-36-36

Acinetobacter ursingii (T); LUH 3792Type; AJ275038

100

0.02

Para se determinar a influência do teor de água (porcentagem de BSW)

presente nos fluidos na diversidade da comunidade bacteriana caracterizada

no presente estudo, as diferentes bibliotecas foram comparadas

estatisticamente utilizando-se o programa webLibshuff. Quando foram obtidos

valores de P superiores a 0,025, foi considerado que as bibliotecas não são

significativamente diferentes, isto é, foi considerado que não houve influência

do teor de BSW na comunidade bacteriana observada. Primeiramente, foram

comparadas as bibliotecas de cada uma das plataformas (P48 x P43), sem

separar as amostras pelo BSW (Tabela 3). Foi observado que a comunidade

bacteriana é significativamente diferente entre as duas plataformas. O mesmo

resultado foi obtido quando as amostras provenientes de P48A e P48B (BSW

60% x BSW 5% respectivamente – mesma plataforma) e as amostras P48A e

P43 25 (BSW 60% x BSW 40% respectivamente – plataformas diferentes)

foram comparadas. Ao contrário, as seqüências provenientes das bibliotecas

P43 25 x P43 36 (BSW 40% x BSW 1% respectivamente – mesma plataforma)

e P48B e P43 36 (BSW 5% x BSW 1% respectivamente – plataformas

diferentes) mostraram-se não são significativamente diferentes (p>0,025)

(Tabela 3).

Tabela 3 – Resultado das análises estatísticas utilizando o programa

webLibshuff.

(*)

As amostras P48 e P43 correspondem à junção de todas as seqüências

obtidas de cada uma das duas plataformas (independente do teor de BSW)

5.3. ISOLAMENTO DAS ESTIRPES A PARTIR DAS DIFERENTES

AMOSTRAS DE FLUIDOS E DETERMINAÇÃO DA CAPACIDADE

DE DEGRADAR ÓLEO CRU

Foram isoladas ao todo 14 estirpes apresentando diferentes morfologias

coloniais, das quatro amostras de fluido produzido estudadas. Foram obtidos

isolados de todas as amostras e a partir dos diferentes meios de cultura

utilizados (LB, TSB e MB). Estes receberam o nome iniciado pelo meio usado

para seu isolamento, seguido da amostra de fluido (36, 25, A e B,

correspondendo respectivamente às amostras P43 36, P43 25, P48A e P48B)

e um numeral. As estirpes isoladas foram coradas pelo método de Gram e se

apresentaram na maioria como bastonetes Gram-positivos. As estirpes LB B-2,

LB 25-1, LB B-1, LB 36-1 e TSB 25-1 apresentaram morfologia celular

característica de cocos ou coco-bacilo e a estirpe MB A1 de bastonete Gram-

negativo (Tabela 4).

Amostras Valores de P

P48 X P43

(*)

0,001

P43 25 X P43 36 0,469

P48A X P48B 0,001

P48A X P43 25 0,001

P48B X P43 36 0,037

Tabela 4 – Caracterização fenotípica das estirpes isoladas.

ESTIRPE CÉLULA GRAM AMOSTRA

TSB B1 Bastonete positiva P48B

TSB 25-1 Coco-Bacilo positiva P43 25

TSB 36-1 Bastonete positiva P43 36

TSB 25-3 Bastonete positiva P43 25

LB A1 Bastonete positiva P48A

LB B2 Coco positiva P48B

LB 25-1 Coco-Bacilo positiva P43 25

LB B1 Coco positiva P48B

LB 36-1 Coco-Bacilo positiva P43 36

LB 25-2 Bastonete positiva P43 25

MB A1 Bastonete negativa P48A

MB 36-1 Bastonete positiva P43 36

MB 25-1 Bastonete positiva P43 25

MB A2 Bastonete positiva P48A

As primeiras letras (TSB, LB ou MB) indicam o meio de cultura utilizado para o

isolamento das estirpes.

Quando as diferentes estirpes foram testadas quanto a sua capacidade

de degradar óleo cru em teste em microplacas, apenas uma única estirpe (TSB

25-1) apresentou capacidade de degradar óleo após 15 dias de incubação

(Figuras 7 A e B).

Figura 7 – Teste de degradação de óleo cru após 1 dia (A) e 15 dias (B) de

incubação. A1, Controle negativo; B1, TSB 36-1; B2, TSB B-1; B3, TSB 25-3;

B4, TSB 25-1; B5, MB A-1; B6, MB A-2; C1, MB 36-1; C2, MB 25-1; C3, LB 25-

2; C4, LB B-2; C5, LB 36-1; C6, LB A-1; D1, LB 25-1; D2, LB B-1.

6. DISCUSSÃO

A natureza e a diversidade das bactérias dos ecossistemas dos

reservatórios de petróleo ainda não são muito conhecidas, mas vem sendo

estudadas por diversos grupos de pesquisa ao redor do mundo. A incorporação

recente dos métodos moleculares possibilitou uma melhor caracterização dos

microrganismos destes ambientes. No presente trabalho, foram utilizadas

abordagens dependentes e independentes de cultivo de microrganismos

utilizando-se amostras de fluido produzido de duas plataformas petrolíferas

brasileiras. A influência do teor de água presente nos fluidos (porcentagem de

BSW) sobre a diversidade da comunidade bacteriana foi determinada por

métodos moleculares. Os resultados foram gerados através da extração de

DNA das amostras de fluidos petrolíferos seguido de amplificação por PCR e

construção de bibliotecas de clones. Já a abordagem clássica de microbiologia

foi baseada no isolamento de estirpes bacterianas das amostras em questão.

A dificuldade de extração de DNA ainda é um fator limitante para estudar

ambientes de reservatórios de petróleo. Muitas metodologias estão sendo

desenvolvidas para tentar concentrar células microbianas das amostras antes

da utilização dos métodos convencionais de extração de DNA. Estudos

recentes (SETTE et al., 2007; TANAKA et al., 2002; VON DER WEID et al.,

2008) utilizaram lavagens da amostra analisada com posterior concentração

das células antes da extração. Tanaka e colaboradores (2002) estudando

bactérias redutoras de sulfato diretamente do óleo e Sette e colaboradores

(2007) trabalhando também com amostras de óleo para o estudo das

comunidades bacterianas utilizaram kits comerciais após a lavagem e

concentração das células. Já von der Weid e colaboradores (2008) trabalhando

com amostras de rocha de reservatórios de petróleo utilizaram o tampão de

Winogradsky para as lavagens e posterior extração com um método de lise

química (SELDIN & DUBNAU, 1985). Em todos os trabalhos, os autores

obtiveram um DNA suficientemente puro para ser amplificado por PCR

utilizando iniciadores específicos para o gene que codifica o 16S rRNA. No

presente estudo, as amostras de fluidos petrolíferos também foram lavadas

com tampão de Winogradsky para extração de células microbianas presentes

nos mesmos. O produto dessa lavagem foi concentrado por centrifugação e

posteriormente foi realizada a extração de DNA através de metodologia de lise

química seguida de purificação, como descrito por SELDIN & DUBNAU (1985).

O resultado obtido mostrou que essa metodologia foi eficiente para a obtenção

de DNA das amostras de fluidos petrolíferos de boa qualidade, isto é um DNA

isento possíveis inibidores das reações de PCR. A utilização do tampão de

Winogradsky nas lavagens dos fluidos permitiu a separação das células

microbianas do fluido e foi demonstrado que ele foi eficiente e fundamental

para que posteriormente fossem obtidos DNAs que pudessem ser utilizados

nas análises realizadas. Os DNAs extraídos das amostras de fluídos

petrolíferos foram então utilizados para estudar a comunidade microbiana

através de métodos moleculares. Produtos de PCR de tamanho esperado

foram obtidos quando foram utilizados iniciadores baseados no 16S rRNA de

Bacteria.

Diversos estudos mostram que espécies de Pseudomonas são os

microrganismos mais detectados em diferentes ambientes contaminados com

hidrocarbonetos, tanto através de abordagens dependentes de cultivo

(DAGHER et al., 1997; AITKEN et al., 1998; STAPLETON, BRIGHT &

SAYLER, 2000; BELHAJ, DESNOUES & ELMERICH, 2002; SAADOUN, 2002;

BHATTACHARYA et al., 2003; ZOCCA et al., 2004; VACCA, BLEAM &

HICKEY, 2005; MANCERA-LÓPEZ et al., 2007), como através de abordagens

independentes de cultivo (MARGESIN et al., 2003; KAPLAN & KITTS, 2004;

RÖLING et al., 2004; POPP, SCHLÖMANN & MAU, 2006; MARGESIN,

HÄMMERLE & TSCHERKO, 2007). Corroborando com esses estudos, a

análise das seqüências revelou uma predominância do gênero Pseudomonas

em todas as amostras analisadas, demonstrando que este gênero é

extremamete adaptado a este tipo de ambiente (Tabela 2 e Figura 2).

Do mesmo modo, Wang e colaboradores (2008) trabalhando com um

sistema para a comparação e monitoramento da manutenção de bactérias

exógenas e autóctones de sistemas petrolíferos e Li e colaboradores (2006)

trabalhando com a diversidade bacteriana de água produzida utilizaram

métodos moleculares como a amplificação do gene que codifica o 16S rRNA,

clonagem e seqüenciamento e obtiveram em suas bibliotecas uma

predominância do gênero Pseudomonas. Li e colaboradores (2006) obtiveram

uma porcentagem alta de sequências relacionadas com microrganismos não

cultiváveis, além de sequências de outros microrganismos como Burkholderia,

Wolinella e Serratia, mas que possuem menos expressão na comunidade em

termos de porcentagem. Wang e colaboradores (2008) clonaram também uma

porcentagem alta de sequências relacionadas com microrganismos não

cultiváveis, além de sequências relacionadas com Delftia, Acinetobacter,

Comamonas e Burkholderia. Utilizando a mesma abordagem dos trabalhos

citados acima e trabalhando com amostras de fluido de reservatórios de

petróleo, Yamane e colaboradores (2008) clonaram sequências relacionadas

com os gêneros Acinetobacter, Acidovorax, Corynebacterium, Marinobacter,

Pseudomonas, Sphingomonas e também sequências relacionadas com

microrganismos não cultiváveis, porém não foi observada a predominância de

nenhum gênero bacteriano. O presente estudo também utilizou métodos

moleculares como a amplificação do gene que codifica o 16S rRNA, clonagem

e seqüenciamento e foi observada uma predominância do gênero

Pseudomonas (como já citado) em todas as bibliotecas construídas. Foram

obtidas também sequências de outros microrganismos tais como Acidovorax,

Acinetobacter,

Bacillus, Burkholderia, Cedecea, Comamonas,

Dendrosporobacter, Janthinobacterium, Marinobacter,

Ochrobactrum,

Simplicispira, Stenotrophomonas e Tistrella.

Clones e estirpes-tipo do gênero Pseudomonas presentes nas árvores

filogenéticas das amostras P48B e P43-25 (Figuras 4 e 5) demonstraram

pouca similaridade com outras pertencentes ao mesmo gênero (clones: P48B –

7, 8, 11, 18, 20 e 37; P43-25 – 1, 21 e 23). Estes dados sugerem que a região

escolhida (flanqueada pelos iniciadores U968 e L1401 – Tabela 1) deve ser

variável para estes clones e estirpes-tipo do gênero Pseudomonas, e o

tamanho do fragmento trabalhado (400 pares de bases) pode ter sido

insuficiente para permitir um perfeito agrupamento para as seqüências em

questão.

O gênero Janthinobacterium que tem como epécie-tipo a espécie

Janthinobacterium lividum foi encontrado em todas as bibliotecas de clones

construídas no presente estudo, apesar deste gênero não ser citado na

literatura como um constituinte deste tipo de ambiente (KÄMPFER, FALSEN &

BUSSE, 2008). Entretanto, este gênero deve apresentar alguma importância

dentro da biodiversidade dos reservatórios de petróleo, já que foi encontrado

em todas as amostras. O fato deste gênero não ser citado na literatura como

constituinte destes ambientes pode estar relacionado à falta de conhecimento a

respeito da biodiversidade em questão, e por existir uma grande dificuldade de

estudar amostras de reservatórios de petróleo.

O gênero Tistrella que tem como espécie-tipo a espécie Tistrella mobilis

é comumente encontrada em ambientes marinhos (CUI et al., 2008). Foram

obtidas 13 sequências que alinharam preferencialmente com este gênero na

amostra P48A (BSW 60%). Esta amostra possui uma quantidade maior de

água e sedimentos (BSW) do que de óleo, e foi considerada significativamente

diferente de todas as outras analisadas, através dos testes estatísticos

(Libshuff), o que pode explicar a presença deste número alto de clones que

alinharam com seqüências deste gênero nesta amostra. Estas 13 sequências

da amostra P48A que alinharam com seqüências do gênero Tistrella (P48A-2,

P48A-4, P48A-5, P48A-6, P48A-7, P48A-9, P48A-14, P48A-17, P48A-21,

P48A-22, P48A-23, P48A-24, P48A-25) quando comparadas com as

seqüências no banco de dados do RDP (Ribossomal Database Project) não

obtiveram um alinhamento com um valor de similaridade entre as seqüências

que permitisse afirmar com confiabilidade que estas sequências pertencem ao

gênero em questão. Ao analisar a árvore filogenética desta amostra (Figura 3)

os dados corroboram com os obtidos nos alinhamentos, pois estas seqüências

ficam distantes da espécie-tipo mais próxima (Tistrella mobilis). Porém, ao

analisar os alinhamentos obtidos podemos observar (Anexo 1) que em todos

os alinhamentos realizados estas sequências alinham com microrganismos

geralmente encontrados em ambientes marinhos (Tistrella, Ochrobactrum,

Pseudochrobactrum) (KORENBLUM et al., 2010). Análises mais detalhadas

são necessárias para afirmar com precisão, porém os dados obtidos sugerem

que estas seqüências são encontradas em microrganismos isolados de água

produzida (água do mar) (KAEMPFER et al., 2003; CUI et al., 2008;

KORENBLUM et al., 2010), porém trata-se de uma biodiversidade nova,

principalmente em ambientes de reservatórios de petróleo.

Uma parcela relevante das seqüências de todas as amostras (14

sequências) apresentou menos de 97% de identidade com as seqüências dos

bancos de dados: P48A 6 (Tistrella), P48A 32 (Simplicispira), P48A 35

(Marinobacter), P48B 11 (Pseudomonas), P48B 20 (Pseudomonas), P48B 31

(Janthinobacterium), P48B 39 (Pseudomonas), P43-25 1 (Pseudomonas), P43-

25 14 (Janthinobacterium), P43-25 17 (Janthinobacterium), P43-25 19

(Pseudomonas), P43-25 21 (Pseudomonas), P43-36 17 (Stenotrophomonas) e

P43-36 19 (Comamonas) (Figuras 3, 4, 5 e 6). Estes clones têm possibilidade

de representar uma biodiversidade ainda desconhecida, pois nos alinhamentos

foi obtido um percentual de similaridade inferior ao que é necessário para ser

considerado como mesma espécie (97%). Porém, para afirmar com exatidão

que esses clones representam sequências de microrganismos desconhecidos

seria necessário um estudo mais detalhado envolvendo outros genes além do

gene que codifica o rRNA 16S.

As análises realizadas utilizando o programa Libshuff demonstraram que

a comunidade bacteriana das amostras P43-25 (BSW 40%), P43-36 (BSW 1%)

e P48B (BSW 5%) não são significativamente diferentes e que a comunidade

bacteriana da amostra P48A (BSW 60%) é significativamente diferente de

todas as outras comunidades (Tabela 3). Isto é demonstrado através das

comparações feitas entre as comunidades, onde as análises estatísticas deste

programa somente apontam diferenças significativas quando a comunidade da

amostra P48A é comparada com outra comunidade. Nas outras comparações

onde a amostra P48A não está presente o programa não aponta diferenças

significativas. Esta amostra é a que apresenta a maior porcentagem de água e

sedimentos (BSW), possuindo uma quantidade maior de BSW do que de óleo

(BSW 60%). Provavelmente, nas outras amostras a diferença entre as

porcentagens de BSW não foi suficiente para causar mudanças significativas

nas comunidades bacterianas destas amostras apesar da amostra P43-25

apresentar um BSW de 40% e as outras duas amostras serem compostas

praticamente de óleo puro P43-36 (BSW 1%) e P48B (BSW 5%).

A presença de microrganismos degradadores nos reservatórios de

petróleo gera prejuízos grandes para a indústria, pois os primeiros compostos

que são degradados são justamente os que possuem alto valor comercial, o

que diminui muito o valor do produto final. Além disso, quando sofrem

degradação os óleos se tornam mais viscosos, ricos em compostos de enxofre,

resinas, asfaltenos, e metais. Além disso, os valores de acidez são

aumentados com a degradação. A bioprospecção de estirpes degradadoras de

petróleo tem sido colocada como objetivo principal em diversos estudos

(AITKEN et al., 1998; BELHAJ, DESNOUES & ELMERICH, 2002;

BHATTACHARYA et al., 2003; DAGHER et al., 1997; MANCERA-LÓPEZ et al.,

2007; STAPLETON, BRIGHT & SAYLER, 2000; SAADOUN, 2002; SETTE et

al., 2007; TANAKA et al., 2002; ZOCCA et al., 2004; VACCA, BLEAM &

HICKEY, 2005). Existe uma grande dificuldade de isolar estirpes bacterianas

diretamente do petróleo com os métodos existentes. Poucos trabalhos

existentes na literatura isolaram microrganismos diretamente de amostras de

óleo como Tanaka e colaboradores (2002) e Sette e colaboradores (2007).

Ambos utilizaram a mesma abordagem do presente estudo (lavagens da

amostra com aquecimento utilizando tampão) para a obtenção das células

bacterianas. Tanaka e colaboradores (2002) utilizaram um meio de cultura para

microrganismos anaeróbicos descrito por Collins & Widdel (1986) e um Meio

Nutriente comercial (Becton Dickinson, Sparks, MD, USA) e isolou os gêneros

Desulfovibrio, Rhodococcus, Mesorhizobium, Paracoccus, e Acetobacter. Sette

e colaboradores (2007) utilizaram os meios de cultura TSB (Difco), GYM

(Difco), Meio Marinho (Difco), Meio para Acinetobacter (Difco) e o meio BH

(Difco), e isolaram os gêneros Bacillus, Methylobacterium, Acinetobacter,

Achromobacter, Brevibacillus e Dietzia.

No presente trabalho foi utilizada a abordagem descrita acima (lavagens

da amostra com aquecimento utilizando tampão) e os meios de cultura TSB, LB

e MB. A maioria dos microrganismos isolados foi constituída de bactérias

Gram-positivas, o que não era esperado, já que os trabalhos presentes na

literatura e acima citados descrevem o isolamento com maior facilidade de

bactérias Gram-negativas de ambientes contendo petróleo. Esses dados

também não condizem com as análises feitas por métodos independentes de

cultivo, onde foram obtidos em sua maioria seqüências de microrganismos

pertencentes ao gênero Pseudomonas (bactéria Gram-negativa). Uma possível

explicação para esse fato é que os meios de cultura utilizados possam

favorecer o isolamento de bactérias Gram-positivas. Outra hipótese diz respeito

à preservação dos fluidos. Como eles foram mantidos em temperaturas baixas

até a chegada ao laboratório, isso pode também ter favorecido o isolamento de

bactérias Gram-positivas formadoras de endosporos.

Estirpes bacterianas foram isoladas de todas as amostras estudadas.

Uma caracterização mais profunda destas estirpes é fundamental, pois a

obtenção desses dados poderia gerar conhecimento sobre a ecologia do

ambiente estudado. Comparando com outros estudos presentes na literatura e

levando em consideração o ambiente estudado que é citado como um

ambiente pobre em quantidade de microrganismos e de difícil obtenção de

isolados, o número de isolados obtidos é condizente com o de trabalhos

anteriores como Sette e colaboradores (2007) e Tanaka e colaboradores

(2002).

Apenas uma estirpe isolada apresentou potencial de degradação (TSB

25-1) de petróleo no teste em placa de degradação e pode ser uma estirpe

promissora para utilização na biorremediação de ambientes contaminados com

petróleo (Figura 7), porém uma caracterização mais detalhada desta estirpe é

necessária e pode ser realizada em estudos futuros.

7. CONCLUSÕES

• Os Clones pertencentes ao gênero Pseudomonas sp. predominaram em

todas as comunidades bacterianas das quatro amostras de fluido

analisadas das plataforamas P43 (BSW 1 e BSW40) e P48 (BSW 5 e

BSW60).

• Uma estirpe foi isolada das amostras de fluido e possui capacidade de

degradar óleo. Esta estirpe deve ser melhor caracterizada através de

estudos mais detalhados para verificar a capacidade de ser utilizada

para remediação de ambientes contaminados com petróleo.

• A biodiversidade da amostra P48A (BSW 60%) foi a única que foi

significativamente diferente das outras amostras. Nesta amostra foi

encontrada uma quantidade significante de clones (13 clones) que

alinharam com seqüências de microrganismos típicos da água do mar.

Neste estudo 3 das 4 comunidades bacterianas

estudadas apresentaram clones cujas seqüências alinharam com

seqüências de microrganismos geralmente encontrados em ambientes

contendo petróleo, e uma comunidade onde uma parcela das

seqüências alinhou com seqüências de microrganismos encontrados no

óleo e outra parcela alinhou seqüências de microrganismos encontrados

no na água do mar.

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Anexo I – Alinhamento das Seqüências com os Bancos de Dados do

GenBank (usando a Ferramenta blastn) e RDP (Ribossomal Database

Project

>P48A-1

TGTACAAGACCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGA

GTTGCAGACTGCGATCCGGACTACGATCGGTTTTATGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACC

GACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTT

GTCACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGA

CTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAA

377 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU111706.1

Pseudomonas putida strain T25 16S ribosomal RNA

gene, partial sequence

681 681 100% 0.0 100%

EU111693.1

Pseudomonas putida strain T12 16S ribosomal RNA

gene, partial sequence

677 677 99% 0.0 100%

GU198119.1

Pseudomonas fluorescens strain LMG 14571 16S

ribosomal RNA gene, partial sequence

675 675 100% 0.0 99%

Lineage:

Results for Query Sequence: P48A-1, 371 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.981 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145154 - not_calculated 0.981 1434 Pseudomonas poae (T); type strain: DSM 14936 = LMG

21465; P 527/13; AJ492829

S000395040 - not_calculated 0.981 1369 Pseudomonas umsongensis (T); Ps 3-10; AF468450

S000428381 - not_calculated 0.981 1398 Pseudomonas jessenii (T); CIP 105274; AF068259

S000499514 - not_calculated 0.970 1424 Pseudomonas extremorientalis (T); KMM 3447; AF405328

> P48A-2

TTGGTACAAGACCCGGGAACGTATTCACCGCGGCGCGCTGATCCGCGATTACTAGTAATTCCAGCTTCATGTACTC

GAGTTGCAGAGTACAATCCGAACTGAGGCATTTTTTTAGGATTTGCTTAACGTCGCCGTCTTGCTTCCTGTTGTAAA

TGCCATTGTAGCACGTGTGTAGCCCAACACGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCTT

ATCACTGGCAGTTCTTTCAGAGTGCCCAACTAAACGATGGCAACTAAAAGTGAGGGTTGCGCTCGTTGCGGGATTT

AACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTTCGTCCGGCCGAACCGAAAAC

377 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EF573256.1

Uncultured bacterium clone S23_1355 16S ribosomal

RNA gene, partial sequence

666 666 99% 0.0 99%

GU119485.1

Uncultured bacterium clone Reef_B13 16S ribosomal

RNA gene, partial sequence

661 661 99% 0.0 99%

GU119347.1

Uncultured bacterium clone Reef_D19 16S ribosomal

RNA gene, partial sequence

661 661 99% 0.0 99%

Lineage:

Results for Query Sequence: P48A-2, 365 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Alphaproteobacteria (5)

order Rhodospirillales (1)

family Rhodospirillaceae (1)

genus Tistrella (1)

S000021075 - not_calculated 0.578 1361 Tistrella mobilis (T); AB071665

order Rhizobiales (4)

family Brucellaceae (4)

genus Ochrobactrum (2)

S000133471 - not_calculated 0.573 1366 Ochrobactrum gallinifaecis (T); type strain: Iso 196;

AJ519939

S000446569 - not_calculated 0.567 1396 Ochrobactrum grignonense (T); type strain:OgA9a;

AJ242581

genus Pseudochrobactrum (2)

S000623991 - not_calculated 0.570 1340 Pseudochrobactrum saccharolyticum (T); type strain:

CCUG 33852; AM180484

S000623992 - not_calculated 0.570 1391 Pseudochrobactrum asaccharolyticum (T); type strain:

CCUG 46016; AM180485

> P48A-3

TGTTACAAGACCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCG

AGTTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTAC

CGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGT

TTGTCACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGG

GACTTAACCCAACATCTCACGGCACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCA

378 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU111693.1

Pseudomonas putida strain T12 16S ribosomal RNA

gene, partial sequence

673 673 100% 0.0 99%

EU482922.1

Pseudomonas sp. CB19 16S ribosomal RNA gene,

partial sequence

672 672 99% 0.0 99%

EU534581.1

Uncultured bacterium clone nbt181d07 16S ribosomal

RNA gene, partial sequence

672 672 99% 0.0 99%

Lineage:

Results for Query Sequence: P48A-3, 372 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000108891 - not_calculated 0.954 1409 Pseudomonas kilonensis (T); 520-20; AJ292426

S000145156 - not_calculated 0.954 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.954 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000395042 - not_calculated 0.954 1366 Pseudomonas koreensis (T); Ps 9-14; AF468452

S000428331 - not_calculated 0.954 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P48A-4

TGTGTACAAGACCCGGGAACGTATTCACCGCGGCGCGCTGATCCGCGATTACTAGTAATTCCAGCTTCATGTACTC

GAGTTGCAGAGTACAATCCGAACTGAGGCATTTTTTTAGGATTTGCTTAACGTCGCCGTTTTGCTTCCTGTTGTAAA

TGCCATTGTAGCACGTGTGTAGCCCAACACGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCTT

ATCACTGGCAGTTCTTTCAGAGTGCCCAACTAAACGATGGCAACTAAAAGTGAGGGTTGCGCTCGTTGCGGGACTT

AACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTTCGTCCGGCCGAACCGAAAAC

377 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU805332.1

Uncultured bacterium clone 6C233335 16S ribosomal

RNA gene, partial sequence

675 675 100% 0.0 99%

EU804761.1

Uncultured bacterium clone 6C232721 16S ribosomal

RNA gene, partial sequence

675 675 100% 0.0 99%

EU804717.1

Uncultured bacterium clone 6C232675 16S ribosomal

RNA gene, partial sequence

675 675 100% 0.0 99%

Lineage:

Results for Query Sequence: P48A-4, 364 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Alphaproteobacteria (5)

order Rhodospirillales (1)

family Rhodospirillaceae (1)

genus Tistrella (1)

S000021075 - not_calculated 0.604 1361 Tistrella mobilis (T); AB071665

order Rhizobiales (4)

family Brucellaceae (4)

genus Ochrobactrum (2)

S000133471 - not_calculated 0.599 1366 Ochrobactrum gallinifaecis (T); type strain: Iso 196;

AJ519939

S000446569 - not_calculated 0.591 1396 Ochrobactrum grignonense (T); type strain:OgA9a;

AJ242581

genus Pseudochrobactrum (2)

S000623991 - not_calculated 0.593 1340 Pseudochrobactrum saccharolyticum (T); type strain:

CCUG 33852; AM180484

S000623992 - not_calculated 0.593 1391 Pseudochrobactrum asaccharolyticum (T); type strain:

CCUG 46016; AM180485

> P48A-5

TGTGGTACAAGACCCGGGAACGTATTCACCGNGGCGCGCTAATGCGCGATTACTAGTAATTCCAGCTTCATGTACT

CGAGTTGCAGAGTACAATCCGAACTGAGGCATTTTTTTAGGATTTGCTTAACGTCGCCGTTTTGCTTCCTGTTGTAA

ATGCCATTGTAGCACGTGTGTAGCCCAACACGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCT

TATCACTGGCAGTTCTTTCAGAGTGCCCAACTAAACGATGGCAACTAAAGGTGAGGGTTGCGCTCGTTGCGGGACT

TAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTTCGTCCGGCCGAACCGAAAAC

378 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU805332.1

Uncultured bacterium clone 6C233335 16S ribosomal

RNA gene, partial sequence

654 654 98% 0.0 98%

EU804761.1

Uncultured bacterium clone 6C232721 16S ribosomal

RNA gene, partial sequence

654 654 98% 0.0 98%

EU804717.1

Uncultured bacterium clone 6C232675 16S ribosomal

RNA gene, partial sequence

654 654 98% 0.0 98%

Lineage:

Results for Query Sequence: P48A-5, 358 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Alphaproteobacteria (5)

order Rhodospirillales (1)

family Rhodospirillaceae (1)

genus Tistrella (1)

S000021075 - not_calculated 0.587 1361 Tistrella mobilis (T); AB071665

order Rhizobiales (4)

family Brucellaceae (4)

genus Ochrobactrum (2)

S000133471 - not_calculated 0.578 1366 Ochrobactrum gallinifaecis (T); type strain: Iso 196;

AJ519939

S000446569 - not_calculated 0.567 1396 Ochrobactrum grignonense (T); type strain:OgA9a;

AJ242581

genus Pseudochrobactrum (2)

S000623991 - not_calculated 0.570 1340 Pseudochrobactrum saccharolyticum (T); type strain:

CCUG 33852; AM180484

S000623992 - not_calculated 0.570 1391 Pseudochrobactrum asaccharolyticum (T); type strain:

CCUG 46016; AM180485

> P48A-6

AACCTTACAACACTTGACATGGCCGCCGCCACATTGNGATGATTAAGTTTTCGGTTCGGCCGGACGAAACACAGGT

GCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCACTTTTAGT

TGCCATCGTTTAGTTGGGCACTCTGAAAGAACTGCCAGTGATAAGCTGGAGGAAGGTGGGGATGACGTCAAGTCC

TCATGGTTCTTACGTGTCCCCGCTACACACGTGCTACAATGGCATTTACAACAGGAAGCAAAACGGCGACGTTAAG

CAAATCCTAAAAAAATGCCTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAGCTGGAATTACTAGTAA

376 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU805332.1

Uncultured bacterium clone 6C233335 16S ribosomal

RNA gene, partial sequence

592 592 100% 4e-166

95%

EU804761.1

Uncultured bacterium clone 6C232721 16S ribosomal

RNA gene, partial sequence

592 592 100% 4e-166

95%

EU804717.1

Uncultured bacterium clone 6C232675 16S ribosomal

RNA gene, partial sequence

592 592 100% 4e-166

95%

Lineage:

Results for Query Sequence: P48A-6, 356 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Alphaproteobacteria (5)

order Rhodospirillales (3)

family Rhodospirillaceae (1)

genus Tistrella (1)

S000021075 not_calculated 0.531 1361 Tistrella mobilis (T); AB071665

family Acetobacteraceae (2)

genus Acidocella (2)

S000413726 not_calculated 0.508 1299 Acidocella aminolytica (T); 101; D30771

S000413729 not_calculated 0.503 1332 Acidocella facilis (T); ATCC 35904; D30774

order Rhizobiales (2)

family Aurantimonadaceae (1)

genus Martelella (1)

S000504762 not_calculated 0.500 1410 Martelella mediterranea (T); MACL11; AY649762

family Brucellaceae (1)

genus Ochrobactrum (1)

S000133471 not_calculated 0.503 1366 Ochrobactrum gallinifaecis (T); type strain: Iso 196;

AJ519939

> P48A-7

TGTGTACAAGACCCGGGAACGTATCACCCCGGCGCGCAGTGCCGTGATTACTAGTAATTCCAGCTTCATGTACTCG

AGTTGCAGAGTACAATCCGAACTGAGGCATTTTTTTAGGATTTGCTTAACGTCGCCGTTTTGCTTCCTGTTGTAAAT

GCCATTGTAGCACGTGTGTAGCCCAACACGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCTTA

TCACTGGCTGTTCTTTCACACCTGCCCAACTAAACGATGGCAACTAAAAGTGAGGGTTGCGCTCGTTGCGGGACTT

AACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTTCGTCCGGCCGAACCGAAAAC

377 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU805332.1

Uncultured bacterium clone 6C233335 16S ribosomal

RNA gene, partial sequence

625 625 100% 6e-176

97%

EU804761.1

Uncultured bacterium clone 6C232721 16S ribosomal

RNA gene, partial sequence

625 625 100% 6e-176

97%

EU804717.1

Uncultured bacterium clone 6C232675 16S ribosomal

RNA gene, partial sequence

625 625 100% 6e-176

97%

Lineage:

Results for Query Sequence: P48A-7, 364 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Alphaproteobacteria (5)

order Rhodospirillales (2)

family Rhodospirillaceae (1)

genus Tistrella (1)

S000021075 - not_calculated 0.560 1361 Tistrella mobilis (T); AB071665

family Acetobacteraceae (1)

genus Acidocella (1)

S000413726 - not_calculated 0.527 1299 Acidocella aminolytica (T); 101; D30771

order Rhizobiales (3)

family Brucellaceae (1)

genus Ochrobactrum (1)

S000133471 - not_calculated 0.525 1366 Ochrobactrum gallinifaecis (T); type strain: Iso 196;

AJ519939

family Bradyrhizobiaceae (2)

genus Bosea (2)

S000390729 - not_calculated 0.533 1368 Bosea eneae (T); 34614; AF288300

S000390735 - not_calculated 0.533 1373 Bosea vestrisii (T); 34635; AF288306

> P48A-8

TACAGACCCGGGAACTGTTCACGACGACATGCCGATGCGCTATTACTAGCGATTCCAACTTCATGCAGTCGAGTTG

CAGACTACAATCCGGACTACAATACACTTTCTGCGATTAGCTCCCCCTCGCGGGTTGGCGGCGCTCTGTATGTACC

ATTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCA

CCGGCAGTCTCATTAGACCGCCCTTTCGTAGCAACTAATGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTACTGGTTCTCTTTCGAGCACTCC

368 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU540499.1

Uncultured bacterium clone nbt116e10 16S ribosomal

RNA gene, partial sequence

618 618 100% 9e-174

97%

EU540498.1

Uncultured bacterium clone nbt116e09 16S ribosomal

RNA gene, partial sequence

618 618 100% 9e-174

97%

EU540432.1

Uncultured bacterium clone nbt115d08 16S ribosomal

RNA gene, partial sequence

618 618 100% 9e-174

97%

Lineage:

Results for Query Sequence: P48A-8, 356 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Betaproteobacteria (5)

order Burkholderiales (5)

family Oxalobacteraceae (5)

genus Duganella (1)

S000022017 - not_calculated 0.817 1375 Duganella zoogloeoides (T); IAM12670; D14256

genus Janthinobacterium (2)

S000006620 - not_calculated 0.885 1393 Janthinobacterium lividum (T); DSM 1522T; Y08846

S000008887 - not_calculated 0.857 1397 Janthinobacterium agaricidamnosum (T); W1r3T; Y08845

genus Massilia (1)

S000515996 - not_calculated 0.817 1410 Massilia dura (T); 16; AY965998

genus Herminiimonas (1)

S000574631 - not_calculated 0.809 1342 Herminiimonas fonticola (T); S-94; AY676462

> P48A-9

TGTGTACAAGACCCGGGAACGTATTCACCGCGGCGCGCTGATCCGCGATTACTAGTAATTCCAGCTTCATGTACTC

GAGTTGCAGAGTACAATCCGAACTGAGGCATTTTTTTAGGATTTGCTTAACGTCGCCGTTTTGCTTCCTGTTGTAAA

TGCCATTGTAGCACGTGTGTAGCCCAACACGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCTT

ATCACTGGCAGTTCTTTCAGAGTGCCCAACTAAACGATGGCAACTAAAAGTGAGGGTTGCGCTCGTTGCGGGACTT

AACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTTCGTCCGGCCGAACCGAAAAC

377 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU805332.1

Uncultured bacterium clone 6C233335 16S ribosomal

RNA gene, partial sequence

675 675 100% 0.0 99%

EU804761.1

Uncultured bacterium clone 6C232721 16S ribosomal

RNA gene, partial sequence

675 675 100% 0.0 99%

EU804717.1

Uncultured bacterium clone 6C232675 16S ribosomal

RNA gene, partial sequence

675 675 100% 0.0 99%

Lineage:

Results for Query Sequence: P48A-9, 364 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Alphaproteobacteria (5)

order Rhodospirillales (1)

family Rhodospirillaceae (1)

genus Tistrella (1)

S000021075 - not_calculated 0.604 1361 Tistrella mobilis (T); AB071665

order Rhizobiales (4)

family Brucellaceae (4)

genus Ochrobactrum (2)

S000133471 - not_calculated 0.599 1366 Ochrobactrum gallinifaecis (T); type strain: Iso 196;

AJ519939

S000446569 - not_calculated 0.591 1396 Ochrobactrum grignonense (T); type strain:OgA9a;

AJ242581

genus Pseudochrobactrum (2)

S000623991 - not_calculated 0.593 1340 Pseudochrobactrum saccharolyticum (T); type strain:

CCUG 33852; AM180484

S000623992 - not_calculated 0.593 1391 Pseudochrobactrum asaccharolyticum (T); type strain:

CCUG 46016; AM180485

> P48A-10

TGTTACAAGACCCGGGAACGTATTCACCCCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCG

AGTTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTAC

CGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGT

TTGTCACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGG

GACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAA

378 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU111693.1

Pseudomonas putida strain T12 16S ribosomal RNA

gene, partial sequence

673 673 100% 0.0 99%

EU482922.1

Pseudomonas sp. CB19 16S ribosomal RNA gene,

partial sequence

672 672 99% 0.0 99%

DQ480483.1

Uncultured bacterium clone G24 16S ribosomal RNA

gene, partial sequence

672 672 99% 0.0 99%

Lineage:

Results for Query Sequence: P48A-10, 372 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000108891 - not_calculated 0.960 1409 Pseudomonas kilonensis (T); 520-20; AJ292426

S000145156 - not_calculated 0.960 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.960 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000428331 - not_calculated 0.960 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

S000428332 - not_calculated 0.960 1422 Pseudomonas cedrina (T); CFML 96-198; AF064461

> P48A-11

TGTGTACAAGACCCGGGAACGTATCACCGCGACATTCTGTATTCGCGATTACTAGCGATTCCGACTTCACGCAGTC

GAGTTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGT

ACCGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCG

GTTTGTCACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACG

GGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACC

379 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU482922.1

Pseudomonas sp. CB19 16S ribosomal RNA gene,

partial sequence

670 670 100% 0.0 99%

DQ480483.1

Uncultured bacterium clone G24 16S ribosomal RNA

gene, partial sequence

670 670 100% 0.0 99%

DQ469202.1

Uncultured bacterium clone SX2-3 16S ribosomal

RNA gene, partial sequence

670 670 100% 0.0 99%

Lineage:

Results for Query Sequence: P48A-11, 373 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000108891 - not_calculated 0.957 1409 Pseudomonas kilonensis (T); 520-20; AJ292426

S000145156 - not_calculated 0.957 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.957 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000395042 - not_calculated 0.957 1366 Pseudomonas koreensis (T); Ps 9-14; AF468452

S000428331 - not_calculated 0.957 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P48A-12

TGTGTACAAGAGCCGGGAACGTATCACCGCGACATTCTGTTTCGCGATTACTAGCGATTCCGACTTCACGCAGTCG

AGTTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTAC

CGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGT

TTGTCACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGG

GACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAA

378 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU482922.1

Pseudomonas sp. CB19 16S ribosomal RNA gene,

partial sequence

666 666 100% 0.0 99%

EU169190.1

Pseudomonas tolaasii strain B35 16S ribosomal RNA

gene, partial sequence

666 666 100% 0.0 99%

EU169189.1

Pseudomonas to

laasii strain B34 16S ribosomal RNA

gene, partial sequence

666 666 100% 0.0 99%

Lineage:

Results for Query Sequence: P48A-12, 372 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000108891 - not_calculated 0.949 1409 Pseudomonas kilonensis (T); 520-20; AJ292426

S000145156 - not_calculated 0.949 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.949 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000395042 - not_calculated 0.949 1366 Pseudomonas koreensis (T); Ps 9-14; AF468452

S000428331 - not_calculated 0.949 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P48A-13

TGTGTACAAGACCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTC

GAGTTGCAGACTGCGATCCGGACTACGATCGGTTTTATGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTA

CCGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGG

TTTGTCACCGGCTGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGG

GACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCAGCTGTCTCAATGTTCCCGAAGGCACCAA

379 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU111706.1

Pseudomonas putida strain T25 16S ribosomal RNA

gene, partial sequence

675 675 100% 0.0 99%

GU198119.1

Pseudomonas fluorescens strain LMG 14571 16S

ribosomal RNA gene, partial sequence

670 670 100% 0.0 99%

GU198118.1

Pseudomonas fluorescens strain LMG 14566 16S

ribosomal RNA gene, partial sequence

670 670 100% 0.0 99%

Lineage:

Results for Query Sequence: P48A-13, 371 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.949 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145154 - not_calculated 0.949 1434 Pseudomonas poae (T); type strain: DSM 14936 = LMG

21465; P 527/13; AJ492829

S000395040 - not_calculated 0.949 1369 Pseudomonas umsongensis (T); Ps 3-10; AF468450

S000428381 - not_calculated 0.949 1398 Pseudomonas jessenii (T); CIP 105274; AF068259

S000499514 - not_calculated 0.938 1424 Pseudomonas extremorientalis (T); KMM 3447; AF405328

> P48A-14

TGTGTACAAGACCCGGGAACGTATTCACCGCGGCGCGCTGATCCGCGATTACTAGTAATTCCAGCTTCATGTACTC

GAGTTGCAGAGTACAATCCGAACTGAGGCATTTTTTTAGGATTTGCTTAACGTCGCCGTTTTGCTTCCTGTTGTAAA

TGCCATTGTAGCACGTGTGTAGCCCAACACGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCTT

ATCACTGGCAGTTCTTTCAGAGTGCCCAACTAAACGATGGCAACTAAAAGTGAGGGTTGCGCTCGTTGCGGGACTT

AACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTTCGTCCGGCCGAACCGAAAAC

377 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU805332.1

Uncultured bacterium clone 6C233335 16S ribosomal

RNA gene, partial sequence

675 675 100% 0.0 99%

EU804761.1

Uncultured bacterium clone 6C232721 16S ribosomal

RNA gene, partial sequence

675 675 100% 0.0 99%

EU804717.1

Uncultured bacterium clone 6C232675 16S ribosomal

RNA gene, partial sequence

675 675 100% 0.0 99%

Lineage:

Results for Query Sequence: P48A-14, 364 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Alphaproteobacteria (5)

order Rhodospirillales (1)

family Rhodospirillaceae (1)

genus Tistrella (1)

S000021075 - not_calculated 0.604 1361 Tistrella mobilis (T); AB071665

order Rhizobiales (4)

family Brucellaceae (4)

genus Ochrobactrum (2)

S000133471 - not_calculated 0.599 1366 Ochrobactrum gallinifaecis (T); type strain: Iso 196;

AJ519939

S000446569 - not_calculated 0.591 1396 Ochrobactrum grignonense (T); type strain:OgA9a;

AJ242581

genus Pseudochrobactrum (2)

S000623991 - not_calculated 0.593 1340 Pseudochrobactrum saccharolyticum (T); type strain:

CCUG 33852; AM180484

S000623992 - not_calculated 0.593 1391 Pseudochrobactrum asaccharolyticum (T); type strain:

CCUG 46016; AM180485

> P48A-15

GTTGTCAAGACCCGGGAACGTATTCCCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGA

GTTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTAC

CGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGT

TTGTCACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGG

GACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAA

377 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU540284.1

Uncultured bacterium clone nbt113h05 16S ribosomal

RNA gene, partial sequence

668 668 99% 0.0 99%

FJ976580.1

Pseudomonas sp. LCR71 16S ribosomal RNA gene,

partial sequence

666 666 99% 0.0 99%

EU482924.1

Pseudomonas sp. CB21 16S ribosomal RNA gene,

partial sequence

666 666 98% 0.0 99%

Lineage:

Results for Query Sequence: P48A-15, 371 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000108891 - not_calculated 0.962 1409 Pseudomonas kilonensis (T); 520-20; AJ292426

S000145156 - not_calculated 0.962 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.962 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000395042 - not_calculated 0.962 1366 Pseudomonas koreensis (T); Ps 9-14; AF468452

S000428888 - not_calculated 0.968 1342 Pseudomonas thivervalensis (T); SBK26; CFBP 11261T;

AF100323

> P48A-16

TGTGTACAGACCCGGGAACGTATCACGCCGACATTCTAATTGCGTATTACTAGCGATTCCGACTTCACGCAGTCGA

GTTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTAC

CGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGT

TTGTCACCGGCAGTCTCCTTAGACTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGG

ACTCAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAA

377 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU539713.1

Uncultured bacterium clone nbt105a03 16S ribosomal

RNA gene, partial sequence

632 632 100% 4e-178

97%

EU538869.1

Uncultured bacterium clone nbt95e11 16S ribosomal

RNA gene, partial sequence

632 632 100% 4e-178

97%

EU538239.1

Uncultured bacterium clone nbt83f05 16S ribosomal

RNA gene, partial sequence

632 632 100% 4e-178

97%

Lineage:

Results for Query Sequence: P48A-16, 370 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000108891 - not_calculated 0.886 1409 Pseudomonas kilonensis (T); 520-20; AJ292426

S000145156 - not_calculated 0.889 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.889 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000395042 - not_calculated 0.884 1366 Pseudomonas koreensis (T); Ps 9-14; AF468452

S000428331 - not_calculated 0.889 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P48A-17

TTGTACAAGACCCGGGAACGTATCAAACCGGCGCGCAGTGCCGTGATTACTAGTAATTCCAGCTTCATGTACTCGA

GTTGCAGAGTACAATCCGAACTGAGGCATTTTTTTAGGATTTGCTTAACGTCGCCGTTTTGCTTCCTGTTGTAAATG

CCATTGTAGCACGTGTGTAGCCCAACACGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCTTAT

CACTGGCTTTTCTTTCAGAGTGCCCAACTAAACGATGGCAACTAAAAGTGAGGGTTGCGCTCGTTGCGGGACTTAA

CCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTTCGTCCGGCCGAACCGAAAAG

375 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU805332.1

Uncultured bacterium clone 6C233335 16S ribosomal

RNA gene, partial sequence

621 621 99% 7e-175

97%

EU804761.1

Uncultured bacterium clone 6C232721 16S ribosomal

RNA gene, partial sequence

621 621 99% 7e-175

97%

EU804717.1

Uncultured bacterium clone 6C232675 16S ribosomal

RNA gene, partial sequence

621 621 99% 7e-175

97%

Lineage:

Results for Query Sequence: P48A-17, 362 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Alphaproteobacteria (5)

order Rhodospirillales (3)

family Rhodospirillaceae (2)

genus Magnetospirillum (1)

S000008159 - not_calculated 0.541 1376 Magnetospirillum gryphiswaldense (T); MSR-1/DSM 6361;

Y10109

genus Tistrella (1)

S000021075 - not_calculated 0.552 1361 Tistrella mobilis (T); AB071665

family Acetobacteraceae (1)

genus Acidocella (1)

S000413726 - not_calculated 0.539 1299 Acidocella aminolytica (T); 101; D30771

order Rhizobiales (2)

family Brucellaceae (2)

genus Ochrobactrum (2)

S000133471 - not_calculated 0.539 1366 Ochrobactrum gallinifaecis (T); type strain: Iso 196;

AJ519939

S000446569 - not_calculated 0.536 1396 Ochrobactrum grignonense (T); type strain:OgA9a;

AJ242581

> P48A-18

TGTGTACAAGACCCGGGAACGTATTCCNCGTGACATTCTGATTCACGATTACTAGCGATTCCGACTTCACGCAGTC

GAGTTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCAGCTTGGCAACCCTCTGTA

CCGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGG

TTTGTCACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGG

GACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAA

379 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

FJ581004.1

Uncultured Pseudomonas sp. isolate DGGE gel band

lx28 16S ribosomal RNA gene, partial sequence

663 663 100% 0.0 98%

FJ581002.1

Uncultured Pseudomonas sp. isolate DGGE gel band

lx26 16S ribosomal RNA gene, partial sequence

663 663 100% 0.0 98%

EU482922.1

Pseudomonas sp. CB19 16S ribosomal RNA gene,

partial sequence

663 663 100% 0.0 98%

Lineage:

Results for Query Sequence: P48A-18, 366 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000014863 - not_calculated 0.932 1375 Pseudomonas lundensis (T); ATCC 49968T; AB021395

S000108891 - not_calculated 0.932 1409 Pseudomonas kilonensis (T); 520-20; AJ292426

S000145156 - not_calculated 0.932 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.932 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000395042 - not_calculated 0.932 1366 Pseudomonas koreensis (T); Ps 9-14; AF468452

> P48A-19

TGTGTACAGACCCGGGAACGTATCACCGCGACATTCATTATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCG

AGTTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTAC

CGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGT

TCGTCACTGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGG

GACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAA

378 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU539713.1

Uncultured bacterium clone nbt105a03 16S ribosomal

RNA gene, partial sequence

650 650 100% 0.0 98%

EU538869.1

Uncultured bacterium clone nbt95e11 16S ribosomal

RNA gene, partial sequence

650 650 100% 0.0 98%

EU538239.1

Uncultured bacterium clone nbt83f05 16S ribosomal

RNA gene, partial sequence

650 650 100% 0.0 98%

Lineage:

Results for Query Sequence: P48A-19, 372 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000108891 - not_calculated 0.909 1409 Pseudomonas kilonensis (T); 520-20; AJ292426

S000145156 - not_calculated 0.909 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.909 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000395042 - not_calculated 0.909 1366 Pseudomonas koreensis (T); Ps 9-14; AF468452

S000428331 - not_calculated 0.909 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P48A-20

TGTGTACAAGACGCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTC

GAGTTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGT

ACCGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCG

GTTTGTCACCGTCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACG

GGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACC

379 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU482922.1

Pseudomonas sp. CB19 16S ribosomal RNA gene,

partial sequence

675 675 100% 0.0 99%

DQ480483.1

Uncultured bacterium clone G24 16S ribosomal RNA

gene, partial sequence

675 675 100% 0.0 99%

DQ469202.1

Uncultured bacterium clone SX2-3 16S ribosomal

RNA gene, partial sequence

675 675 100% 0.0 99%

Lineage:

Results for Query Sequence: P48A-20, 373 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000108891 - not_calculated 0.957 1409 Pseudomonas kilonensis (T); 520-20; AJ292426

S000145156 - not_calculated 0.960 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.960 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000428331 - not_calculated 0.960 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

S000428332 - not_calculated 0.960 1422 Pseudomonas cedrina (T); CFML 96-198; AF064461

> P48A-21

TGTGTACAAGACCCGGGAACGTATTCACCGCGGCGCGCTGTATCCGCGATTACTAGTAATTCCAGCTTCATGTACT

CGAGTTGCAGAGTACAATCCGAACTGAGGCATTTTTTTAGGATTTGCTTAACGTCGCCGTTTTGCTTCCTGTTGTAA

ATGCCATTGTAGCACGTGTGTAGCCCAACACGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCT

TATCACTGGCAGTTCTTTCAGAGTGCCCAACTAAACGATGGCAACTAAAAGTGAGGGTTGCGCTCGTTGCGGGACT

TAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTTCGTCCGGCCGAACCGAAAAC

378 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU805332.1

Uncultured bacterium clone 6C233335 16S ribosomal

RNA gene, partial sequence

670 670 100% 0.0 99%

EU804761.1

Uncultured bacterium clone 6C232721 16S ribosomal

RNA gene, partial sequence

670 670 100% 0.0 99%

EU804717.1

Uncultured bacterium clone 6C232675 16S ribosomal

RNA gene, partial sequence

670 670 100% 0.0 99%

Lineage:

Results for Query Sequence: P48A-21, 365 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Alphaproteobacteria (5)

order Rhodospirillales (1)

family Rhodospirillaceae (1)

genus Tistrella (1)

S000021075 - not_calculated 0.600 1361 Tistrella mobilis (T); AB071665

order Rhizobiales (4)

family Brucellaceae (4)

genus Ochrobactrum (2)

S000133471 - not_calculated 0.595 1366 Ochrobactrum gallinifaecis (T); type strain: Iso 196;

AJ519939

S000446569 - not_calculated 0.586 1396 Ochrobactrum grignonense (T); type strain:OgA9a;

AJ242581

genus Pseudochrobactrum (2)

S000623991 - not_calculated 0.581 1340 Pseudochrobactrum saccharolyticum (T); type strain:

CCUG 33852; AM180484

S000623992 - not_calculated 0.581 1391 Pseudochrobactrum asaccharolyticum (T); type strain:

CCUG 46016; AM180485

> P48A-22

TGTTGTACAAGACCCGGGAACGTATTCCCCGCGGCGCGCTGATCCGCGATTACTAGTAATTCCAGCTTCATGTACT

CGAGTTGCAGAGTACAATCCGAACTGAGGCATTTTTTTAGGATTTGCTTAACGTCGCCGTTTTGCTTCCTGTTGTAA

ATGCCATTGTAGCACGTGTGTAGCCCAACACGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCT

TATCACTGTTTATTTCTTTCAGAGTGCCCAACTAAACGATGGCAACTAAAAGTGAGGGTTGCGCTCGTTGCGGGACT

TAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTTCGTCCGGCCGAACCGAAAAC

379 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU805332.1

Uncultured bacterium clone 6C233335 16S ribosomal

RNA gene, partial sequence

648 648 99% 0.0 98%

EU804761.1

Uncultured bacterium clone 6C232721 16S ribosomal

RNA gene, partial sequence

648 648 99% 0.0 98%

EU804717.1

Uncultured bacterium clone 6C232675 16S ribosomal

RNA gene, partial sequence

648 648 99% 0.0 98%

Lineage:

Results for Query Sequence: P48A-22, 365 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Alphaproteobacteria (5)

order Rhodospirillales (2)

family Rhodospirillaceae (1)

genus Tistrella (1)

S000021075 - not_calculated 0.581 1361 Tistrella mobilis (T); AB071665

family Acetobacteraceae (1)

genus Stella (1)

S000351836 - not_calculated 0.556 1368 Stella vacuolata (T); DSM5901; AJ535711

order Rhizobiales (3)

family Brucellaceae (3)

genus Ochrobactrum (1)

S000133471 - not_calculated 0.559 1366 Ochrobactrum gallinifaecis (T); type strain: Iso 196;

AJ519939

genus Pseudochrobactrum (2)

S000623991 - not_calculated 0.553 1340 Pseudochrobactrum saccharolyticum (T); type strain:

CCUG 33852; AM180484

S000623992 - not_calculated 0.553 1391 Pseudochrobactrum asaccharolyticum (T); type strain:

CCUG 46016; AM180485

> P48A-23

TGTGTACAAGACCCGGGAACGTATTCACCGCGGCGCGCCAATTCGCGATTAGTAGTAATTCCAGCTTCATGTACTC

GAGTTGCAGAGTACAATCCGAACTGAGGCATTTTTTTAGGATTTGCTTAACGTCGCCGTTTTGCTTCCTGTTGTAAA

CGCCATTGTAGCACGTGTGTAGCCCAACACGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCTT

ATCACTGGCAGTTCTTTCAGACTGCCCAACTAAACGATGGCAACTAAAAGTGAGGGTTGCGCTCGTTGCGGGACTT

AACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTTCGTCCGGCCGAACCGAAAAC

377 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU805332.1

Uncultured bacterium clone 6C233335 16S ribosomal

RNA gene, partial sequence

648 648 100% 0.0 98%

EU804761.1

Uncultured bacterium clone 6C232721 16S ribosomal

RNA gene, partial sequence

648 648 100% 0.0 98%

EU804717.1

Uncultured bacterium clone 6C232675 16S ribosomal

RNA gene, partial sequence

648 648 100% 0.0 98%

Lineage:

Results for Query Sequence: P48A-23, 364 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Alphaproteobacteria (5)

order Rhodospirillales (1)

family Rhodospirillaceae (1)

genus Tistrella (1)

S000021075 - not_calculated 0.582 1361 Tistrella mobilis (T); AB071665

order Rhizobiales (4)

family Brucellaceae (4)

genus Ochrobactrum (2)

S000133471 - not_calculated 0.558 1366 Ochrobactrum gallinifaecis (T); type strain: Iso 196;

AJ519939

S000446569 - not_calculated 0.549 1396 Ochrobactrum grignonense (T); type strain:OgA9a;

AJ242581

genus Pseudochrobactrum (2)

S000623991 - not_calculated 0.555 1340 Pseudochrobactrum saccharolyticum (T); type strain:

CCUG 33852; AM180484

S000623992 - not_calculated 0.558 1391 Pseudochrobactrum asaccharolyticum (T); type strain:

CCUG 46016; AM180485

> P48A-24

TGTGTACAAGACCCGGGAACGTATTCACCGCGGCGCGCTGATCCGCGATTACTAGTAATTCCAGCTTCATGTACTC

GAGTTGCAGAGTACAATCCGAACTGAGGCATTTTTTTAGGATTTGCTTAACGTCGCCGTTTTGCTTCCTGTTGTAAA

TGCCATTGTAGCACGTGTGTAGCCCAACACGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCTT

ATCACTGGCAGTTCTTTCAGAGTGCCCAACTAAACGATGGCAACTAAAAGTGAGGGTTGCGCTCGTTGCGGGACTT

AACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTTCGTCCGGCCGAACCGAAAAC

377 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU805332.1

Uncultured bacterium clone 6C233335 16S ribosomal

RNA gene, partial sequence

675 675 100% 0.0 99%

EU804761.1

Uncultured bacterium clone 6C232721 16S ribosomal

RNA gene, partial sequence

675 675 100% 0.0 99%

EU804717.1

Uncultured bacterium clone 6C232675 16S ribosomal

RNA gene, partial sequence

675 675 100% 0.0 99%

Lineage:

Results for Query Sequence: P48A-24, 364 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Alphaproteobacteria (5)

order Rhodospirillales (1)

family Rhodospirillaceae (1)

genus Tistrella (1)

S000021075 - not_calculated 0.604 1361 Tistrella mobilis (T); AB071665

order Rhizobiales (4)

family Brucellaceae (4)

genus Ochrobactrum (2)

S000133471 - not_calculated 0.599 1366 Ochrobactrum gallinifaecis (T); type strain: Iso 196;

AJ519939

S000446569 - not_calculated 0.591 1396 Ochrobactrum grignonense (T); type strain:OgA9a;

AJ242581

genus Pseudochrobactrum (2)

S000623991 - not_calculated 0.593 1340 Pseudochrobactrum saccharolyticum (T); type strain:

CCUG 33852; AM180484

S000623992 - not_calculated 0.593 1391 Pseudochrobactrum asaccharolyticum (T); type strain:

CCUG 46016; AM180485

> P48A-25

TGGTACAAGACCCGGGAACGTATTCACCGCGGCGCGCTGATCCGCGATTACTAGTAATTCCAGCTTCATGTACTCG

AGCTGCAGAGTACAATCCGAACTGAGGCATTTTTTTAGGATTTGCTTAACGTCGACGTTTTGCTTCCTGTTGTAAAT

GCCATTGTAGCACGTGTGTAGCCCAACACGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCTTA

TCACTGGCAGTTCTTTCAGAGTGCCCAACTAAACGATGGCAACTAAAAGTGAGGGTTGCGCTCGTTGCGGGACTTA

ACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTTCGTCCGGCCGAACCGAAAAC

376 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU805332.1

Uncultured bacterium clone 6C233335 16S ribosomal

RNA gene, partial sequence

661 661 99% 0.0 99%

EU804761.1

Uncultured bacterium clone 6C232721 16S ribosomal

RNA gene, partial sequence

661 661 99% 0.0 99%

EU804717.1

Uncultured bacterium clone 6C232675 16S ribosomal

RNA gene, partial sequence

661 661 99% 0.0 99%

Lineage:

Results for Query Sequence: P48A-25, 362 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Alphaproteobacteria (5)

order Rhodospirillales (1)

family Rhodospirillaceae (1)

genus Tistrella (1)

S000021075 - not_calculated 0.583 1361 Tistrella mobilis (T); AB071665

order Rhizobiales (4)

family Aurantimonadaceae (1)

genus Martelella (1)

S000504762 - not_calculated 0.566 1410 Martelella mediterranea (T); MACL11; AY649762

family Brucellaceae (3)

genus Ochrobactrum (1)

S000133471 - not_calculated 0.572 1366 Ochrobactrum gallinifaecis (T); type strain: Iso 196;

AJ519939

genus Pseudochrobactrum (2)

S000623991 - not_calculated 0.566 1340 Pseudochrobactrum saccharolyticum (T); type strain:

CCUG 33852; AM180484

S000623992 - not_calculated 0.566 1391 Pseudochrobactrum asaccharolyticum (T); type strain:

CCUG 46016; AM180485

> P48A-26

TGTGTACAAGAGCCCGGGAACGTATTCCCGCGGCATGCAGTGCCGTGATTACTAGCGATTCCAGCTTCATGTAGG

CGAGTTGCAGCCTACAATCCGAACTGAGAACGGTTTTATGAGATTAGCTCCACCTCGCGGTCTTGCAGCTCTTTGT

ACCGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGG

TTTGTCACCGGTTGTCACCTTAGAGTGCCCAACTGAATGATGGCAACTAAGATCAAGGGTTGCGCTCGTTGCGGGA

CTTAACCCAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTCACTCTGCTCCCGAAGGAGAAG

376 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EF100618.1

Bacillus cereus strain HS-N25 16S ribosomal RNA

gene, partial sequence

639 639 100% 3e-180

97%

GU186119.1

Bacillus cereus strain IHB B 1380 16S ribosomal

RNA gene, partial sequence

637 637 100% 1e-179

97%

GU011953.1

Bacillus cereus strain NBST8 16S ribosomal RNA

gene, partial sequence

637 637 100% 1e-179

97%

Lineage:

Results for Query Sequence: P48A-26, 367 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Firmicutes (5)

class "Bacilli" (5)

order Bacillales (5)

family Bacillaceae (5)

subfamily "Bacillaceae 1" (5)

supergenus Bacillus (5)

genus Bacillus d (3)

S000016288 - not_calculated 0.894 1446 Bacillus weihenstephanensis (T); DSM11821;

AB021199

S000021267 - not_calculated 0.896 1406 Bacillus thuringiensis (T); ATCC10792; AF290545

S000388279 - not_calculated 0.866 1427 Bacillus mycoides (T); 6462; AF155956

genus Bacillus e (1)

S000429573 - not_calculated 0.796 1419 Bacillus marisflavi (T); TF-11; AF483624

genus Bacillus k (1)

S000383485 - not_calculated 0.837 1435 Bacillus funiculus (T); NAF001; AB049195

> P48A-27

TGTACAAGACCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGA

GTTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTAC

CGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTCCCTCCGGT

TTGTCACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGG

GACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAA

377 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU482922.1

Pseudomonas sp. CB19 16S ribosomal RNA gene,

partial sequence

675 675 100% 0.0 99%

DQ480483.1

Uncultured bacterium clone G24 16S ribosomal RNA

gene, partial sequence

675 675 100% 0.0 99%

DQ469202.1

Uncultured bacterium clone SX2-3 16S ribosomal

RNA gene, partial sequence

675 675 100% 0.0 99%

Lineage:

Results for Query Sequence: P48A-27, 370 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000108891 - not_calculated 0.965 1409 Pseudomonas kilonensis (T); 520-20; AJ292426

S000145156 - not_calculated 0.965 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.965 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000395042 - not_calculated 0.965 1366 Pseudomonas koreensis (T); Ps 9-14; AF468452

S000428331 - not_calculated 0.965 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P48A-28

TGTGTACAGACCCGGGAACGTATCACCGCGGCGCGCTGATCCGCGATTACTAGTAATTCCAGCTTCATGTACTCGA

GTTGCAGAGTACAATCCGAACTGAGGCATTTTTTTAGGATTTGCTTAACGTCGCCGTTTTGCTTCCTGTTGTAAATG

CCATTGTAGCACGTGTGTAGCCCAACACGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCTTAT

CACTGGCAGTTCTTTCAGAGTGCCCAACTAAACGATGGCAACTAAAAGTGAGGGTTGCGCTCGTTGCGGGACTTAA

CCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTTCGTCCGGCCGAAACGAAAAC

375 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU805332.1

Uncultured bacterium clone 6C233335 16S ribosomal

RNA gene, partial sequence

655 655 100% 0.0 98%

EU804761.1

Uncultured bacterium clone 6C232721 16S ribosomal

RNA gene, partial sequence

655 655 100% 0.0 98%

EU804717.1

Uncultured bacterium clone 6C232675 16S ribosomal

RNA gene, partial sequence

655 655 100% 0.0 98%

Lineage:

Results for Query Sequence: P48A-28, 362 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Alphaproteobacteria (5)

order Rhodospirillales (1)

family Rhodospirillaceae (1)

genus Tistrella (1)

S000021075 - not_calculated 0.572 1361 Tistrella mobilis (T); AB071665

order Rhizobiales (4)

family Brucellaceae (4)

genus Ochrobactrum (2)

S000133471 - not_calculated 0.577 1366 Ochrobactrum gallinifaecis (T); type strain: Iso 196;

AJ519939

S000446569 - not_calculated 0.569 1396 Ochrobactrum grignonense (T); type strain:OgA9a;

AJ242581

genus Pseudochrobactrum (2)

S000623991 - not_calculated 0.575 1340 Pseudochrobactrum saccharolyticum (T); type strain:

CCUG 33852; AM180484

S000623992 - not_calculated 0.575 1391 Pseudochrobactrum asaccharolyticum (T); type strain:

CCUG 46016; AM180485

> P48A-29

TGTACAGACCCGGGAACGTATTCACCGCCACATTCTGATTCTCGATTACTAGCGATTCCGACTTCACGCAGTCGAG

TTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACC

GACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTT

GTCACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGA

CTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAA

376 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU539407.1

Uncultured bacterium clone nbt238d01 16S ribosomal

RNA gene, partial sequence

666 666 99% 0.0 99%

EU539713.1

Uncultured bacterium clone nbt105a03 16S ribosomal

RNA gene, partial sequence

664 664 100% 0.0 99%

EU539322.1

Uncultured bacterium clone nbt102g10 16S ribosomal

RNA gene, partial sequence

664 664 100% 0.0 99%

Lineage:

Results for Query Sequence: P48A-29, 370 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000108891 - not_calculated 0.941 1409 Pseudomonas kilonensis (T); 520-20; AJ292426

S000145156 - not_calculated 0.941 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.941 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000395042 - not_calculated 0.941 1366 Pseudomonas koreensis (T); Ps 9-14; AF468452

S000428331 - not_calculated 0.941 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P48A-30

TGTGTACAAGACCCGGGAACGTATTCACCGCGACCTGCTGATTCGCTATTACTAGCGATTCCGACTTCACGCAGTC

GAGTTGCAGACTGCGATCCGGACTACGATCGGTTTTAAGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTA

CCGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGG

TTTGTCACCGGCAGTCTCCTTAGAGTGCCCACCATGACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACG

GGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACC

379 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU130312.1

Uncultured bacterium clone 100 16S ribosomal RNA

gene, partial sequence

666 666 100% 0.0 98%

GU198127.1

Pseudomonas fluorescens strain LMG 14677 16S

ribosomal RNA gene, partial sequence

661 661 100% 0.0 98%

GU198112.1

Pseudomonas fluorescens strain LMG 5848 16S

ribosomal RNA gene, partial sequence

661 661 100% 0.0 98%

Lineage:

Results for Query Sequence: P48A-30, 373 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.903 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145154 - not_calculated 0.903 1434 Pseudomonas poae (T); type strain: DSM 14936 = LMG

21465; P 527/13; AJ492829

S000428195 - not_calculated 0.920 1422 Pseudomonas libanensis (T); CIP 105460; AF057645

S000428763 - not_calculated 0.922 1363 Pseudomonas chlororaphis subsp. aureofaciens (T);

ATCC 13985; AF094722

S001171509 - not_calculated 0.920 1445 Pseudomonas lini (T); CFBP 5737; AY035996

> P48A-31

AACTTTACCAGGCCTTGACATCCAATGAACTTTCTAGAGATAGATTGGTGCCTTCGGGAACATTGAGACAGGTCCTG

CATGGCTGTCGTCAGCTCGTGTCGTGAGATATTGGGTTAAGTCCCGTAACGAGCGCAACCCTTGTCCTTAGTTACC

AGCACGTACTGGTGGGCACTCTAAGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCAT

CATGGCCCTTACGGCCTGGGCTACACACGTGCTACAATGGTCGGTACAAAGGGTTGCCAAGCCGCGAGGTGGAG

CTAATCCCATAAAACCGATCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGTGAAGTCGGAATCGCTAGTAA

375 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU469659.1

Uncultured bacterium clone FF_-

aag84f02 16S ribosomal

RNA gene, partial sequence

663 663 100% 0.0 99%

FN563404.1

Uncultured bacterium partial 16S rRNA gene, clone

Bacteria-BeetSilage-C

659 659 100% 0.0 98%

GQ448888.1

Uncultured bacterium clone calf32_10wks_grp1_G09

16S ribosomal RNA gene, partial sequence

659 659 100% 0.0 98%

Lineage:

Results for Query Sequence: P48A-31, 368 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000002852 not_calculated 0.921 1436 Pseudomonas psychrophila (T); E-3; AB041885

S000005898 not_calculated 0.899 1429 Pseudomonas azotoformans (T); IAM1603; D84009

S000013342 not_calculated 0.916 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145154 not_calculated 0.916 1434 Pseudomonas poae (T); type strain: DSM 14936 = LMG

21465; P 527/13; AJ492829

S000428774 not_calculated 0.935 1373 Pseudomonas fragi (T); ATCC 4973; AF094733

> P48A-32

TGTGGCAATACCCGGGTACACGTCTTCACCGCTACCATGCTGCTCCGCGCTTACTAGCGCTTCCGACTTCACGCAG

TCGAGTTGCATCCTGCGCTCCGGACTACGCCTGGCTTTATGGGATTAGCTCCCCCTCGCGGGTTGACAACCCTTTG

TACCAGCCATTGTATGACGTGTGTAGCCCCACCTATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCG

GTTTGTCACCGGCAGTCCCATTAGAGTGCTCAACTAAATGTAGCAACTAATGGCAAGGGTTGCGCTCGTTGCGGGA

CTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTACGGCTCTCTTTCGAGCACTCC

380 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU771834.1

Uncultured bacterium clone AFYEL_aaj65h12 16S

ribosomal RNA gene, partial sequence

592 592 100%

4e-

166

95%

EU465125.1

Uncultured bacterium clone AFYEL_aaj68d08 16S

ribosomal RNA gene, partial sequence

592 592 100%

4e-

166

95%

EU465005.1

Uncultured bacterium clone AFYEL_aaj66e10 16S

ribosomal RNA gene, partial sequence

592 592 100%

4e-

166

95%

Lineage:

Results for Query Sequence: P48A-32, 369 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Betaproteobacteria (5)

order Burkholderiales (5)

family Comamonadaceae (5)

genus Comamonas (2)

S000428528 - not_calculated 0.756 1414 Comamonas terrigena (T); IMI 359870; AF078772

S000436037 - not_calculated 0.753 1444 Comamonas testosteroni (T); M11224

genus Giesbergeria (1)

S000610620 - not_calculated 0.762 1396 Giesbergeria kuznetsovii (T); D-412; AY780907

genus Simplicispira (2)

S000428511 - not_calculated 0.759 1423 Simplicispira psychrophila (T); LMG 5408; AF078755

S000428513 - not_calculated 0.772 1354 Simplicispira metamorpha (T); LMG 4339; AF078757

> P48A-33

TGTGTACAGACCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCG

AGTTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTAC

CGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTAGACGTCATCCCCACCTTCCTCCGGT

TTGTCACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGG

GACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAA

378 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU539713.1

Uncultured bacterium clone nbt105a03 16S ribosomal

RNA gene, partial sequence

673 673 100% 0.0 99%

EU538869.1

Uncultured bacterium clone nbt95e11 16S ribosomal

RNA gene, partial sequence

673 673 100% 0.0 99%

EU538239.1

Uncultured bacterium clone nbt83f05 16S ribosomal

RNA gene, partial sequence

673 673 100% 0.0 99%

Lineage:

Results for Query Sequence: P48A-33, 372 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000108891 - not_calculated 0.960 1409 Pseudomonas kilonensis (T); 520-20; AJ292426

S000145156 - not_calculated 0.960 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.960 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000395042 - not_calculated 0.960 1366 Pseudomonas koreensis (T); Ps 9-14; AF468452

S000428331 - not_calculated 0.960 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

>P48A-34

TGTGTACAAGACCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTC

GAGTTGCAGACTGCGATCCGGACTACGGTCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGT

ACCGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCG

GCTTGTCACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACG

GGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACC

379 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU482922.1

Pseudomonas sp. CB19 16S ribosomal RNA gene,

partial sequence

675 675 100% 0.0 99%

DQ480483.1

Uncultured bacterium clone G24 16S ribosomal RNA

gene, partial sequence

675 675 100% 0.0 99%

DQ469202.1

Uncultured bacterium clone SX2-3 16S ribosomal

RNA gene, partial sequence

675 675 100% 0.0 99%

Lineage:

Results for Query Sequence: P48A-34, 372 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000108891 - not_calculated 0.952 1409 Pseudomonas kilonensis (T); 520-20; AJ292426

S000145156 - not_calculated 0.946 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.946 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000395042 - not_calculated 0.946 1366 Pseudomonas koreensis (T); Ps 9-14; AF468452

S000428331 - not_calculated 0.946 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

>P48A-35

TGTGTACAGACCCGGGAACGTATTCCCGCGGCATGCTGATCCTGCGATTACTAGCAATTCCAACTTCATGCACTCG

AGTTGCAGAGTGCAATCCGAACTGAGATATCTTTTTAGGATTTGCTTGACGTCGCCATCTTGCTTCCTATTGTAAAT

GCCATTGTAGCACGTGTGTAGCCCAACACGTAAGGGCCATGATGACCTGACGTCATCCCCACCTTCCTCCGGTTTG

CCACCGGCAGTCTCCCTGGAGTTCTCAGCATTACCTGCTAGCAACCAGGGATAGGGGTTGCGCTCGTTACGGGAC

TTAACCCAACATCTCACGACACGAGCTGACGACGGCCATGCAGCACCTGTCTCAGAGTTCCCGAAGGCACCAA

377 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EF379547.1

Uncultured bacterium clone TLC-FL4-51 16S

ribosomal RNA gene, partial sequence

506 506 100%

4e-

140

90%

DQ396166.1

Uncultured organism clone ctg_NISA247 16S

ribosomal RNA gene, partial sequence

491 491 100%

9e-

136

89%

GU130405.1

Uncultured archaeon clone 302 16S ribosomal RNA

gene, partial sequence

482 482 99%

5e-

133

89%

Lineage:

Results for Query Sequence: P48A-35, 367 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Alteromonadales (5)

family Incertae sedis 7 (5)

genus Marinobacter (5)

S000015722 - not_calculated 0.649 1429 Marinobacter hydrocarbonoclasticus (T); VT8; AJ000726

S000382445 - not_calculated 0.649 1404 Marinobacter hydrocarbonoclasticus (T); MBIC1303

(=ATCC27132); AB019148

S000400179 - not_calculated 0.635 1450 Marinobacter excellens (T); KMM 3809; AY180101

S000420325 - not_calculated 0.624 1379 Marinobacter daepoensis (T); SW-156; AY517633

S000429467 - not_calculated 0.619 1416 Marinobacter litoralis (T); SW-45; AF479689

>P48B-1

CAAGACCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTG

TAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGAC

CATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTC

ACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTA

ACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATC

TCTAGAA

386 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU482922.1

Pseudomonas sp. CB19 16S ribosomal RNA gene,

partial sequence

691 691 100% 0.0 99%

DQ480483.1

Uncultured bacterium clone G24 16S ribosomal RNA

gene, partial sequence

691 691 100% 0.0 99%

DQ469202.1

Uncultured bacterium clone SX2-3 16S ribosomal

RNA gene, partial sequence

691 691 100% 0.0 99%

Lineage:

Results for Query Sequence: P48B-1, 380 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.950 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.968 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.968 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.953 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.968 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

>P48B-2

CAAGACCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTG

CAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGAC

CATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGCCATCCCCACCTTCCTCCGGTTTGTC

ACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTCGCGCTCGTTACGGGACTT

AACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTAT

CTCTAGAA

386 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU482922.1

Pseudomonas sp. CB19 16S ribosomal RNA gene,

partial sequence

688 688 100% 0.0 99%

EU540036.1

Uncultured bacterium clone nbt112b06 16S ribosomal

RNA gene, partial sequence

688 688 100% 0.0 99%

EU535413.1

Uncultured bacterium clone nbt02f03 16S ribosomal

RNA gene, partial sequence

688 688 100% 0.0 99%

Lineage:

Results for Query Sequence: P48B-2, 380 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.932 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.950 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.950 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.934 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.950 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P48B-3

CAAGACCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTG

CAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGAC

CATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGCCATCCCCACCTTCCTCCGGTTTGTC

ACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTCGCGCTCGTTACGGGACTT

AACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTAT

CTCTAGAA

386 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU482922.1

Pseudomonas sp. CB19 16S ribosomal RNA gene,

partial sequence

688 688 100% 0.0 99%

EU540036.1

Uncultured bacterium clone nbt112b06 16S ribosomal

RNA gene, partial sequence

688 688 100% 0.0 99%

EU535413.1

Uncultured bacterium clone nbt02f03 16S ribosomal

RNA gene, partial sequence

688 688 100% 0.0 99%

Lineage:

Results for Query Sequence: P48B-3, 380 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.932 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.950 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.950 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.934 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.950 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

>P48B-4

CAAGACCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTG

CAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGAC

CATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTC

ACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTA

ACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATC

TCTAGAA

386 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU482922.1

Pseudomonas sp. CB19 16S ribosomal RNA gene,

partial sequence

697 697 100% 0.0 100%

DQ480483.1

Uncultured bacterium clone G24 16S ribosomal RNA

gene, partial sequence

697 697 100% 0.0 100%

DQ469202.1

Uncultured bacterium clone SX2-3 16S ribosomal

RNA gene, partial sequence

697 697 100% 0.0 100%

Lineage:

Results for Query Sequence: P48B-4, 380 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.968 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.987 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.987 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.971 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.987 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

>P48B-5

CAAGACCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTG

CAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGAC

CATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTC

ACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTA

ACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATC

TCTAGAA

386 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU482922.1

Pseudomonas sp. CB19 16S ribosomal RNA gene,

partial sequence

697 697 100% 0.0 100%

DQ480483.1

Uncultured bacterium clone G24 16S ribosomal RNA

gene, partial sequence

697 697 100% 0.0 100%

DQ469202.1

Uncultured bacterium clone SX2-3 16S ribosomal

RNA gene, partial sequence

697 697 100% 0.0 100%

Lineage:

Results for Query Sequence: P48B-5, 380 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.968 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.987 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.987 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.971 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.987 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

>P48B-6

CAAGACCCGGGAACGTATTCACCGTGACATTCTGATTCACGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTG

CAGACTGCGATCCGGACTACGATCGGTTTTATGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTTTGTACCGACC

ATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCA

CCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAA

CCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCT

CTAGAA

386 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU291336.1

Uncultured bacterium clone WSMA39 16S ribosomal

RNA gene, partial sequence

691 691 100% 0.0 99%

FN563404.1

Uncultured bacterium partial 16S rRNA gene, clone

Bacteria-BeetSilage-C

691 691 100% 0.0 99%

GQ448888.1

Uncultured bacterium clone calf32_10wks_grp1_G09

16S ribosomal RNA gene, partial sequence

691 691 100% 0.0 99%

Lineage:

Results for Query Sequence: P48B-6, 380 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000002852 - not_calculated 0.974 1436 Pseudomonas psychrophila (T); E-3; AB041885

S000008929 - not_calculated 0.958 1433 Pseudomonas taetrolens (T); IAM1653; D84027

S000013342 - not_calculated 0.934 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000014863 - not_calculated 0.958 1375 Pseudomonas lundensis (T); ATCC 49968T; AB021395

S000428774 - not_calculated 0.987 1373 Pseudomonas fragi (T); ATCC 4973; AF094733

> P48B-7

CAAGACCCGGGAACGTATTCACCGCAGCAATGCTGATCTGCGATTACTAGCGATTCCGACTTCATGGAGTCGAGTT

GCAGACTCCAATCCGGACTGAGATAGGGTTTCTGGGATTGGCTTACCGTCGCCGGCTTGCAGCCCTCTGTCCCTA

CCATTGTAGTACGTGTGTAGCCCTGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGT

CACCGGCGGTCTCCTTAGAGTTCCCACCATTACGTGCTGGCAACTAAGGACAAGGGTTGCGCTCGTTGCGGGACT

TAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTCGAGTTCCCGAAGGCACCAATCC

ATCTCTGGAA

387 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GQ894723.1

Stenotrophomonas maltophilia strain NKY-3 16S

ribosomal RNA gene, partial sequence

699 699 100% 0.0 100%

FJ976542.1

Stenotrophomonas sp. LCR33 16S ribosomal RNA

gene, partial sequence

699 699 100% 0.0 100%

EF033231.1

Uncultured bacterium clone JH-YT40 16S ribosomal

RNA gene, partial sequence

699 699 100% 0.0 100%

Lineage:

Results for Query Sequence: P48B-7, 377 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Xanthomonadales (5)

family Xanthomonadaceae (5)

genus Stenotrophomonas (5)

S000000936 - not_calculated 0.976 1382 Pseudomonas pictorum (T); ATCC 23328T; AB021392

S000007629 - not_calculated 0.987 1345 Pseudomonas geniculata (T); ATCC 19374T; AB021404

S000010261 - not_calculated 0.971 1400 Pseudomonas hibiscicola (T); ATCC 19867T; AB021405

S000381870 - not_calculated 0.987 1384 Stenotrophomonas maltophilia (T); ATCC 13637T;

AB008509

S000390459 - not_calculated 0.952 1434 Stenotrophomonas acidaminiphila (T); AMX19; AF273080

> P48B-8

CAAGACCCGGGAACGTATTCACCGCGACATGCTGATCCGCGATTACTAGCGATTCCAACTTCATGCAGTCGAGTTG

CAGACTACAATCCGGACTACGATACACTTTCTGCGATTAGCTCCCCCTCGCGGGTTGGCGGCGCTCTGTATGTACC

ATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCA

CCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAA

CCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCT

CTAGAA

386 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU540256.1

Uncultured bacterium clone nbt248b08 16S ribosomal

RNA gene, partial sequence

691 691 100% 0.0 99%

DQ264573.1

Uncultured bacterium clone BANW619 16S ribosomal

RNA gene, partial sequence

688 688 100% 0.0 99%

EU244099.1

Uncultured bacterium clone sl1392 16S ribosomal

RNA gene, partial sequence

664 664 100% 0.0 98%

Lineage:

Results for Query Sequence: P48B-8, 378 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.794 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145154 - not_calculated 0.796 1434 Pseudomonas poae (T); type strain: DSM 14936 = LMG

21465; P 527/13; AJ492829

S000145156 - not_calculated 0.796 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.794 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000499371 - not_calculated 0.796 1399 Pseudomonas costantinii (T); CFBP 5705; HAMBI 2444;

AF374472

> P48B-9

CAAGACCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTG

CAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGAC

CATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTC

ACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTA

ACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATC

TCTAGAA

386 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU482922.1

Pseudomonas sp. CB19 16S ribosomal RNA gene,

partial sequence

697 697 100% 0.0 100%

DQ480483.1

Uncultured bacterium clone G24 16S ribosomal RNA

gene, partial sequence

697 697 100% 0.0 100%

DQ469202.1

Uncultured bacterium clone SX2-3 16S ribosomal

RNA gene, partial sequence

697 697 100% 0.0 100%

Lineage:

Results for Query Sequence: P48B-9, 380 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.968 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.987 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.987 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.971 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.987 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P48B-10

CAAGACCCGGGAACGTATTCACCGCGACATGTTGATCCGCGATTACTAGCGATTCCAACTTCATGCAGTCGAGTTG

CAGACTACAATCCGGACTACGATACACTTTCTGCGATTAGCTCCCCCTCGCGGGTTGGCGGCGCTCTGTATGTACC

ATTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCA

CCGGCAGTCTCATTAGAGTGCCCTTTCGTAGCAACTAATGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTACTGGTTCTCTTTCGAGCACTCCTCAATCTCTCGA

381 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU538946.1

Uncultured bacterium clone nbt95g02 16S ribosomal

RNA gene, partial sequence

688 688 100% 0.0 100%

GQ179708.1

Uncultured Janthinobacterium sp. clone VE21B06 16S

ribosomal RNA gene, partial sequence

682 682 100% 0.0 99%

EU828415.1

Uncultured bacterium clone T6_3_16 16S ribosomal

RNA gene, partial sequence

682 682 100% 0.0 99%

Lineage:

Results for Query Sequence: P48B-10, 373 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Betaproteobacteria (5)

order Burkholderiales (5)

family Oxalobacteraceae (5)

genus Duganella (1)

S000022017 - not_calculated 0.903 1375 Duganella zoogloeoides (T); IAM12670; D14256

genus Herbaspirillum (1)

S000334724 - not_calculated 0.887 1386 Herbaspirillum chlorophenolicum (T); CPW301; AB094401

genus Janthinobacterium (2)

S000006620 - not_calculated 0.960 1393 Janthinobacterium lividum (T); DSM 1522T; Y08846

S000008887 - not_calculated 0.925 1397 Janthinobacterium agaricidamnosum (T); W1r3T; Y08845

genus Herminiimonas (1)

S000574631 - not_calculated 0.882 1342 Herminiimonas fonticola (T); S-94; AY676462

> P48B-11

CAGAGCCGGGAACTGTATTCCCCCAGCACCGCAGTGNTGTGATTACGAGCGATTCCGACTTCATGGAGTCGAGTT

GCAGACTCCAATCCGGACTGAGATAGGGTTTCTGGGATTGGCTTACCGTCGCCGGCTTGCAGCCCTCTGTCCCTA

CCATTGTAGTACGTGTGTAGCCCTGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGT

CACCGGTTGTCTCCTTACCCCTCCCACCATTACGTGCTGGCAACTAAGGACAAGGGTTGCGCTCGTTGCGGGACTT

AACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTCGAGTTCCCGAAGGCACCAATCCA

TCTCTGGAA

386 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GQ379478.1

Uncultured bacterium clone R_0706_50 16S ribosomal

RNA gene, partial sequence

610 610 98%

1e-

171

95%

AY958793.1

Uncultured bacterium clone rRNA020 16S ribosomal

RNA gene, partial sequence

610 610 98%

1e-

171

95%

GU186115.1

Stenotrophomonas maltophilia strain IHB B 1365 16S

ribosomal RNA gene, partial sequence

609 609 99%

5e-

171

95%

Lineage:

Results for Query Sequence: P48B-11, 370 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Xanthomonadales (5)

family Xanthomonadaceae (5)

genus Stenotrophomonas (5)

S000000936 - not_calculated 0.870 1382 Pseudomonas pictorum (T); ATCC 23328T; AB021392

S000007629 - not_calculated 0.876 1345 Pseudomonas geniculata (T); ATCC 19374T; AB021404

S000010261 - not_calculated 0.859 1400 Pseudomonas hibiscicola (T); ATCC 19867T; AB021405

S000381870 - not_calculated 0.876 1384 Stenotrophomonas maltophilia (T); ATCC 13637T;

AB008509

S000390459 - not_calculated 0.838 1434 Stenotrophomonas acidaminiphila (T); AMX19; AF273080

> P48B-12

CAAGAGCCGGGAACGTATTCCAACCGACATTCTAATTGCGTATTACTAGCGATTCCGACTTCACGCAGTCGAGTTG

CAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGAC

CATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTC

ACCGGCTGTCTCCTTAGACTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTA

ACCCAACATCTCACGGCACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATC

TCTAGAA

386 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU482922.1

Pseudomonas sp. CB19 16S ribosomal RNA gene,

partial sequence

641 641 100% 0.0 97%

EU540505.1

Uncultured bacterium clone nbt116f07 16S ribosomal

RNA gene, partial sequence

641 641 100% 0.0 97%

EU534747.1

Uncultured bacterium clone nbt210d04 16S ribosomal

RNA gene, partial sequence

641 641 98% 0.0 97%

Lineage:

Results for Query Sequence: P48B-12, 378 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000145154 - not_calculated 0.865 1434 Pseudomonas poae (T); type strain: DSM 14936 = LMG

21465; P 527/13; AJ492829

S000145156 - not_calculated 0.884 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.881 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.868 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.881 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P48B-13

CAAGACCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTG

CAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGAC

CATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTC

ACCGGCAGTCTCCTTAGAGTGCCCACCATAACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTT

AACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCCAT

CTCTGGAA

386 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

FJ639215.1

Pseudomonas sp. 1b-11 (9) 16S ribosomal RNA gene,

partial sequence

691 691 100% 0.0 99%

FJ432556.1

Uncultured bacterium clone MONS_IW0905_37 16S

ribosomal RNA gene, partial sequence

691 691 100% 0.0 99%

GQ891862.1

Pseudomonas sp. DIC1RS 16S ribosomal RNA gene,

partial sequence

691 691 100% 0.0 99%

Lineage:

Results for Query Sequence: P48B-13, 380 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000010434 - not_calculated 0.987 1360 Pseudomonas corrugata (T); ATCC29736; D84012

S000108891 - not_calculated 0.968 1409 Pseudomonas kilonensis (T); 520-20; AJ292426

S000395042 - not_calculated 0.968 1366 Pseudomonas koreensis (T); Ps 9-14; AF468452

S000428465 - not_calculated 0.987 1426 Pseudomonas migulae (T); CIP 105470; AF074383

S000428886 - not_calculated 0.987 1383 Pseudomonas brassicacearum (T); DBK11; CFBP 11706;

AF100321

> P48B-14

CAAGACCCGGGAACGTATTCCCCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGGAGTCGAGTTG

CAGACTCCGATCCGGACTACGACGCGTTTTAAGGGATTGGCTCACTCTCGCGAGTTGGCAGCCCTCTGTACGCGC

CATTGTAGCACGTGTGTAGCCCTGGCCGTAAGGGCCATGATGACCTGACGTCATCCCCACCTTCCTCCGGTTTGTC

ACCGGCAGTCTCCCTGGAGTTCTCAGCATTACCTGCTAGCAACCAGGGATAGGGGTTGCGCCCGTTACGGGACTT

AACCCAACATCTCACGACACGAGCTGACGACGGCCATGCAGCACCTGTCTCAGAGTTCCCGAAGGCACCAATCCA

TCTCTGGAA

386 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU223380.1

Marinobacter sp. EM493 16S ribosomal RNA gene,

partial sequence

682 682 100% 0.0 99%

GU108529.1

Uncultured Marinobacter sp. clone III36-13 16S

ribosomal RNA, partial sequence

682 682 100% 0.0 99%

FN556391.1

Marinobacter hydrocarbonoclasticus partial 16S rRNA

gene, isolate #6

682 682 100% 0.0 99%

Lineage:

Results for Query Sequence: P48B-14, 378 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Alteromonadales (5)

family Incertae sedis 7 (5)

genus Marinobacter (5)

S000015722 - not_calculated 0.958 1429 Marinobacter hydrocarbonoclasticus (T); VT8; AJ000726

S000382445 - not_calculated 0.958 1404 Marinobacter hydrocarbonoclasticus (T); MBIC1303

(=ATCC27132); AB019148

S000400179 - not_calculated 0.907 1450 Marinobacter excellens (T); KMM 3809; AY180101

S000420325 - not_calculated 0.873 1379 Marinobacter daepoensis (T); SW-156; AY517633

S000429467 - not_calculated 0.857 1416 Marinobacter litoralis (T); SW-45; AF479689

> P48B-15

CAAGACCCGGGAACGTATTCACCGTAGCATTCTGATCTACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTG

CAGACTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCC

ATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCAGTTTATCAC

TGGCAGTCTCCTTTGAGTTCCCGGCCGAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAAC

CCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAGAGTTCCCGAAGGCACCAATCCATCTC

TGGAA

385 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU140078.1

Pantoea sp. XJ3 16S ribosomal RNA gene, partial

sequence

690 690 100% 0.0 99%

GU084209.1

Uncultured gamma proteobacterium clone WCG55 16S

ribosomal RNA gene, partial sequence

690 690 100% 0.0 99%

GQ406570.1

Enterobacter cloacae strain R8-2 16S ribosomal RNA

gene, partial sequence

690 690 100% 0.0 99%

Lineage:

Results for Query Sequence: P48B-15, 375 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Enterobacteriales (5)

family Enterobacteriaceae (5)

genus Cedecea (1)

S000120182 - not_calculated 0.987 1305 Cedecea davisae (T); DSM 4568; AF493976

genus Enterobacter (2)

S000021184 - not_calculated 0.939 1408 Enterobacter cloacae subsp. cloacae (T); ATCC13047T;

AJ251469

S000381739 - not_calculated 0.968 1329 Enterobacter asburiae (T); JCM6051; AB004744

genus Kluyvera (2)

S000000149 - not_calculated 0.923 1364 Kluyvera cryocrescens (T); ATCC33435; AF310218

S000022462 - not_calculated 0.923 1372 Kluyvera ascorbata (T); ATCC 33433T; AF008579

> P48B-16

ACAGAGCCGGGAACGTATCACCGCGACATGCTAATGTCGCGATTACTGAGCGATTCCGACTTCACGCAGTCGAGT

TGCAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCG

ACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTG

TCACCGGTTGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACT

TAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTGCCCGAAGGCACCAATCTA

TCTCTAGAA

387 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

FN377723.1

Pseudomonas sp. VI-1 partial 16S rRNA gene, isolate

VI-1

650 650 99% 0.0 97%

FJ894434.1

Uncultured bacterium clone nbt181a12 16S ribosomal

RNA gene, partial sequence

646 646 98% 0.0 98%

EU534559.1

Uncultured bacterium clone nbt181a12 16S ribosomal

RNA gene, partial sequence

646 646 98% 0.0 98%

Lineage:

Results for Query Sequence: P48B-16, 381 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.866 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.885 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.885 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.869 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.885 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P48B-17

CAAGACCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTG

CAGACTGCGATCCGGACTACGACCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGAC

CATTGTAGCACGTGTGTAGCCCGGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTC

ACCGGCAGTCTCCTTATAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTA

TCCCAACATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTCTCAGTGTTCCCGAACGCCCCAGCCTAT

CTCTAGAA

386 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GQ416050.1

Uncultured Pseudomonas sp. clone F5mar.5 16S

ribosomal RNA gene, partial sequence

652 652 100% 0.0 97%

EU482922.1

Pseudomonas sp. CB19 16S ribosomal RNA gene,

partial sequence

652 652 100% 0.0 97%

EU540494.1

Uncultured bacterium clone nbt116e02 16S ribosomal

RNA gene, partial sequence

652 652 100% 0.0 97%

Lineage:

Results for Query Sequence: P48B-17, 379 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000022197 - not_calculated 0.858 1381 Pseudomonas fluorescens (T); DSM 50090T (type strain);

Z76662

S000145156 - not_calculated 0.871 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.871 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000428331 - not_calculated 0.873 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

S000428332 - not_calculated 0.858 1422 Pseudomonas cedrina (T); CFML 96-198; AF064461

> P48B-18

CAANGACCCGGGAACGTATCACGCCAGCAATGCTATTGCTGTGATTACTAGCGATTCCGACTTCATGGAGTCGAGT

TGCAGACTCCAATCCGGACTGAGATAGGGTTTCTGGGATTGGCTTACCGTCGCCGGCTTGCAGCCCTCTGTCCCT

ACCATTGTAGTACGTGTGTAGCCCTGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTG

TCACCGGTTGTCTCCTTAGACTTCCCACCATTACGTGCTGGCAACTAAGGACAAGGGTTGCGCTCGTTGCGGGACT

TAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTCGAGTTCCCGAAGGCACCAATCC

ATCTCTGGAA

388 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GQ894723.1

Stenotrophomonas maltophilia strain NKY-3 16S

ribosomal RNA gene, partial sequence

643 643 98% 0.0 97%

FJ976542.1

Stenotrophomonas sp. LCR33 16S ribosomal RNA

gene, partial sequence

643 643 98% 0.0 97%

EF033231.1

Uncultured bacterium clone JH-YT40 16S ribosomal

RNA gene, partial sequence

643 643 98% 0.0 97%

Lineage:

Results for Query Sequence: P48B-18, 376 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Xanthomonadales (5)

family Xanthomonadaceae (5)

genus Stenotrophomonas (5)

S000000936 - not_calculated 0.880 1382 Pseudomonas pictorum (T); ATCC 23328T; AB021392

S000007629 - not_calculated 0.891 1345 Pseudomonas geniculata (T); ATCC 19374T; AB021404

S000010261 - not_calculated 0.875 1400 Pseudomonas hibiscicola (T); ATCC 19867T; AB021405

S000381870 - not_calculated 0.891 1384 Stenotrophomonas maltophilia (T); ATCC 13637T;

AB008509

S000390459 - not_calculated 0.856 1434 Stenotrophomonas acidaminiphila (T); AMX19; AF273080

> P48B-19

CAAGACCCGGGAACATATCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGC

AGACTGCGATCCGGACTACGATCGGTTTTATGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCA

TTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCAC

CGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAAC

CCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCCATCTC

TGGAA

385 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

FJ976604.1

Pseudomonas sp. LCR95 16S ribosomal RNA gene,

partial sequence

684 684 100% 0.0 99%

FJ976580.1

Pseudomonas sp. LCR71 16S ribosomal RNA gene,

partial sequence

684 684 100% 0.0 99%

EU629353.1

Uncultured Pseudomonas sp. clone URA1Q1u11_T3

16S ribosomal RNA gene, partial sequence

682 682 100% 0.0 99%

Lineage:

Results for Query Sequence: P48B-19, 379 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000004663 - not_calculated 0.945 1430 Pseudomonas veronii (T); CIP 104663; AF064460

S000395040 - not_calculated 0.963 1369 Pseudomonas umsongensis (T); Ps 3-10; AF468450

S000428381 - not_calculated 0.963 1398 Pseudomonas jessenii (T); CIP 105274; AF068259

S000428763 - not_calculated 0.947 1363 Pseudomonas chlororaphis subsp. aureofaciens (T);

ATCC 13985; AF094722

S000499514 - not_calculated 0.953 1424 Pseudomonas extremorientalis (T); KMM 3447; AF405328

> P48B-20

AACGCGAAGAACCTGTACTGGCCTTGACATCTNGAGAACTACTCGGATATGGATGGGTGCCTTCGGGAACTCGAA

CACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAAGCCTTG

TCCTTAGTTGCCAGCACGTAATGGTGGGAACTCTAAGGAGACCGCCGGTGACAAACCGGAGGAAGGTGGGGATGA

CGTCAAGTCATCATGGCCCTTACGGCCACCGCTACACACGTACTACAATGGTAGGGACAGAGGGCTGCAAGCCGG

CGACGGTAAGCCAATCCCAGAAACCCTATCTCAGTCCGGATTGGAGTCTGCAACTCGACTCCATGA

366 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

FJ493052.1

Pseudomonas sp. 2Y-1 16S ribosomal RNA gene,

partial sequence

603 603 100%

2e-

169

96%

GQ417585.1

Uncultured Stenotrophomonas sp. clone F3feb.1 16S

ribosomal RNA gene, partial sequence

603 603 100%

2e-

169

96%

GQ417327.1

Uncultured Stenotrophomonas sp. clone F2mar.13 16S

ribosomal RNA gene, partial sequence

603 603 100%

2e-

169

96%

Lineage:

Results for Query Sequence: P48B-20, 349 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Xanthomonadales (5)

family Xanthomonadaceae (5)

genus Stenotrophomonas (5)

S000000936 not_calculated 0.874 1382 Pseudomonas pictorum (T); ATCC 23328T; AB021392

S000007629 not_calculated 0.888 1345 Pseudomonas geniculata (T); ATCC 19374T; AB021404

S000010261 not_calculated 0.905 1400 Pseudomonas hibiscicola (T); ATCC 19867T; AB021405

S000381870 not_calculated 0.888 1384 Stenotrophomonas maltophilia (T); ATCC 13637T;

AB008509

S000390459 not_calculated 0.848 1434 Stenotrophomonas acidaminiphila (T); AMX19; AF273080

> P48B-21

CAAGACCCGGGAACGTATTCACCGCGGCATTCAGTCCCGTGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTG

CAGACTCCAATCCGGACTACGATCGGCTTTTTGAGATTAGCATCCTATCGCTAGGTAGCAACCCTTTGTACCGACC

ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCGTCCCCGCCTTCCTCCAGTTTGTCA

CTGTCAGTATCCTTACAGTTCCCACCCGAAGTGCTGGCAAATAAGGAAAAGGGTTGCGCTCGTTGCGGGACTTAAC

CCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTATGTAAGTTCCCGAAGGCACCAATCCATCTC

TGGAA

385 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GQ099225.1

Uncultured bacterium clone nbw507g10c1 16S

ribosomal RNA gene, partial sequence

664 664 99% 0.0 98%

AM889158.1

Uncultured proteobacterium partial 16S rRNA gene,

clone 1'C12

663 663 100% 0.0 98%

FJ968638.1

Arcobacter sp. enrichment culture clone M4 16S

ribosomal RNA gene, partial sequence

663 663 100% 0.0 98%

Lineage:

Results for Query Sequence: P48B-21, 376 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Moraxellaceae (5)

genus Acinetobacter (5)

S000001137 - not_calculated 0.896 1357 Acinetobacter johnsonii (T); DSM 6963; X81663

S000003452 - not_calculated 0.875 1366 Acinetobacter lwoffii (T); DSM 2403; X81665

S000008827 - not_calculated 0.899 1364 Acinetobacter haemolyticus (T); DSM 6962; X81662

S000127903 - not_calculated 0.907 1355 Acinetobacter ursingii (T); LUH 3792Type; AJ275038

S000381579 - not_calculated 0.870 1334 Acinetobacter calcoaceticus (T); ATCC 23055T; DNA

group one; Z93434

> P48B-22

CAAGACCCGGGAACGTATTCACCGCGACATTCAGTGTCTCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTG

CAGACTGCGATCCGGACTACGATCGGTTTTATGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGAC

CATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTC

ACCGTTAGTCTCCTTACAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTA

ACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATC

TCTAGAA

386 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU538202.1

Uncultured bacterium clone nbt82h08 16S ribosomal

RNA gene, partial sequence

663 663 99% 0.0 98%

GU198119.1

Pseudomonas fluorescens strain LMG 14571 16S

ribosomal RNA gene, partial sequence

661 661 100% 0.0 97%

GU198118.1

Pseudomonas fluorescens strain LMG 14566 16S

ribosomal RNA gene, partial sequence

661 661 100% 0.0 97%

Lineage:

Results for Query Sequence: P48B-22, 380 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000005898 - not_calculated 0.903 1429 Pseudomonas azotoformans (T); IAM1603; D84009

S000013342 - not_calculated 0.921 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145154 - not_calculated 0.921 1434 Pseudomonas poae (T); type strain: DSM 14936 = LMG

21465; P 527/13; AJ492829

S000145156 - not_calculated 0.903 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000499371 - not_calculated 0.905 1399 Pseudomonas costantinii (T); CFBP 5705; HAMBI 2444;

AF374472

>P48B-23

CAAGGACCCGGGAACGTATTCCCCGCGACATGCTTATCCGCGATTACTAGCGATTCCAACTTCATGCAGTCGAGTT

GCAGACTACAATCCGGACTACGATACACTTTCTGCGATTAGCTCCCCCTCGCGGGTTGGCGGCGCTCTGTATGTAC

CATTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTC

ACCGGCAGTCTCCTTAGAGTGCCCACCATAACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTT

AACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTAT

CTCTAGAA

387 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

FJ184325.1

Uncultured soil bacterium clone B5_22 16S ribosomal

RNA gene, partial sequence

655 655 98% 0.0 97%

EU540256.1

Uncultured bacterium clone nbt248b08 16S ribosomal

RNA gene, partial sequence

641 641 98% 0.0 97%

AY693816.1

Uncultured gamma proteobacterium clone EUB22 16S

ribosomal RNA gene, partial sequence

637 637 98%

1e-

179

96%

Lineage:

Results for Query Sequence: P48B-23, 379 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Betaproteobacteria (5)

order Burkholderiales (5)

family Oxalobacteraceae (5)

genus Duganella (1)

S000022017 - not_calculated 0.734 1375 Duganella zoogloeoides (T); IAM12670; D14256

genus Janthinobacterium (2)

S000006620 - not_calculated 0.776 1393 Janthinobacterium lividum (T); DSM 1522T; Y08846

S000008887 - not_calculated 0.768 1397 Janthinobacterium agaricidamnosum (T); W1r3T; Y08845

genus Massilia (2)

S000515996 - not_calculated 0.731 1410 Massilia dura (T); 16; AY965998

S000515997 - not_calculated 0.734 1397 Massilia albidiflava (T); 45; AY965999

> P48B-25

CAAGACCCGGGAACGTATCACCGCGACATGCTGATCCGTGATTACTAGCGATTCCAACTTCATGCAGTCGAGTTGC

AGACTACAATCCGGACTACGATACACTTTCTGCGATTAGCTCCCCCTCGCGGGTTGGCGGCGCTCTGTATGTACCA

TTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCAC

CGGCAGTCTCAATAGAGTGCCCTTTCGTAGCAACTAATGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACAT

CTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTACTGGTTCTCTTTCGAGCACTCCCAAATCTCTCCGG

380 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU538484.1

Uncultured bacterium clone nbt230e08 16S ribosomal

RNA gene, partial sequence

675 675 100% 0.0 99%

GQ179711.1

Uncultured Janthinobacterium sp. clone VE8B07 16S

ribosomal RNA gene, partial sequence

670 670 100% 0.0 99%

EU537003.1

Uncultured bacterium clone nbt218e12 16S ribosomal

RNA gene, partial sequence

670 670 100% 0.0 99%

Lineage:

Results for Query Sequence: P48B-25, 373 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Betaproteobacteria (5)

order Burkholderiales (5)

family Oxalobacteraceae (5)

genus Herbaspirillum (2)

S000002236 - not_calculated 0.869 1356 Herbaspirillum rubrisubalbicans (T); ATCC 19308T;

AB021424

S000006848 - not_calculated 0.855 1408 Herbaspirillum huttiense (T); ATCC 14670T; AB021366

genus Janthinobacterium (2)

S000006620 - not_calculated 0.922 1393 Janthinobacterium lividum (T); DSM 1522T; Y08846

S000008887 - not_calculated 0.912 1397 Janthinobacterium agaricidamnosum (T); W1r3T; Y08845

genus Massilia (1)

S000515996 - not_calculated 0.874 1410 Massilia dura (T); 16; AY965998

> P48B-26

CAAGACCGGGAACGTATCACCGCGACATGCTGATCCGCGATTACTAGCGATCCAACTTCATGCAGTCGAGTTGCA

GACTACAATCCGGACTACGATACACTTTCTGCGATTAGCTCCCCCTCGCGGGTTGGCGGCGCTCTGTATGTACCAT

TGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCGCC

GGCAGTCTCATTAGAGTGCCCTTTCGTAGCAACTAATGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATC

TCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTACTGGTTCTCTTTCGAGCACTCCCAAATCTCTCCGG

378 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GQ179711.1

Uncultured Janthinobacterium sp. clone VE8B07 16S

ribosomal RNA gene, partial sequence

659 659 100% 0.0 98%

EU537003.1

Uncultured bacterium clone nbt218e12 16S ribosomal

RNA gene, partial sequence

659 659 100% 0.0 98%

EU536932.1

Uncultured bacterium clone nbt224c08 16S ribosomal

RNA gene, partial sequence

659 659 100% 0.0 98%

Lineage:

Results for Query Sequence: P48B-26, 370 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Betaproteobacteria (5)

order Burkholderiales (5)

family Oxalobacteraceae (5)

genus Herbaspirillum (2)

S000002236 - not_calculated 0.862 1356 Herbaspirillum rubrisubalbicans (T); ATCC 19308T;

AB021424

S000006848 - not_calculated 0.849 1408 Herbaspirillum huttiense (T); ATCC 14670T; AB021366

genus Janthinobacterium (2)

S000006620 - not_calculated 0.919 1393 Janthinobacterium lividum (T); DSM 1522T; Y08846

S000008887 - not_calculated 0.908 1397 Janthinobacterium agaricidamnosum (T); W1r3T; Y08845

genus Massilia (1)

S000515996 - not_calculated 0.868 1410 Massilia dura (T); 16; AY965998

> P48B-27

CAGACCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTACACGCAGTCGAGTTGC

AGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACC

ATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCA

CCGGCAGTCTCCTTAGAGTGCCCACCATAACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTA

ACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATC

TCTAGAA

385 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU482919.1

Pseudomonas sp. CB16 16S ribosomal RNA gene,

partial sequence

688 688 99% 0.0 99%

GU198124.1

Pseudomonas fluorescens strain LMG 14674 16S

ribosomal RNA gene, partial sequence

684 684 99% 0.0 99%

GU198113.1

Pseudomonas fluorescens strain LMG 5849 16S

ribosomal RNA gene, partial sequence

684 684 99% 0.0 99%

Lineage:

Results for Query Sequence: P48B-27, 379 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000145156 - not_calculated 0.953 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000355057 - not_calculated 0.958 1299 Pseudomonas salomonii (T); CFBP 2022; AY091528

S000366445 - not_calculated 0.953 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.971 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.953 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P48B-28

GGCACGGGGGGAACGCGAAGAACCTTACCAGGCCTTGACATCCAATGAACTTTCTAGAGATAGATTGGTGCCTTC

GGGAACATTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAG

CGCAACCCTTGTCCTTAGTTACCAGCACGTAATGGTGGGCACTCTAAGGAGACTGCCGGTGACAAACCGGAGGAA

GGTGGGGATGACGTCAAGTCATCATGGCCCTTACGGCCTGGGCTACACACGTGCTACAATGGTCGGTACAGAGGG

TTGCCAAGCCGCGAGGTGGAGCTAATCCCACAAAACCGATCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGT

377 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

AY365101.1

Uncultured bacterium 17PS16S 16S ribosomal RNA

gene, partial sequence

675 675 100% 0.0 99%

AY365103.1

Uncultured bacterium 19PS16S 16S ribosomal RNA

gene, partial sequence

672 672 100% 0.0 99%

AY365102.1

Uncultured bacterium 18PS16S 16S ribosomal RNA

gene, partial sequence

672 672 100% 0.0 99%

Lineage:

Results for Query Sequence: P48B-28, 370 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 not_calculated 0.962 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 not_calculated 0.976 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 not_calculated 0.976 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366446 not_calculated 0.962 1414 Pseudomonas meridiana (T); type strain: CMS 38;

AJ537602

S000428331 not_calculated 0.976 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P48B-29

CGGGGCGGGGGCACGGGGGGAACGCGAAGAACCTTACCAGGCCTTGACATCCAATGAACTTTCCAGAGATGGAT

TGGTGCCTTCGGGAACATTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTC

CCGTAACGAGCGCAACCCTTGTCCTTAGTTACCAGCACGTTATGGTGGGCACTCTAAGGAGACTGCCGGTGACAA

ACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGGCCTGGGCTACACACGTGCTACAATGGTCG

GTACAGAGGGTTGCCAAGCCGCGAGGTGGAGCTAATCCCACAAAACCGATCGTAGTCCGGATCGCAGTCTGCAAC

TCGACTGCGTGA

386 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

AY365082.1

Pseudomonas sp. Ps8 16S ribosomal RNA gene,

partial sequence

697 697 100% 0.0 100%

AY365102.1

Uncultured bacterium 18PS16S 16S ribosomal RNA

gene, partial sequence

691 691 100% 0.0 99%

AY365099.1

Uncultured bacterium 15PS16S 16S ribosomal RNA

gene, partial sequence

691 691 100% 0.0 99%

Lineage:

Results for Query Sequence: P48B-29, 379 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000010434 not_calculated 0.958 1360 Pseudomonas corrugata (T); ATCC29736; D84012

S000108891 not_calculated 0.939 1409 Pseudomonas kilonensis (T); 520-20; AJ292426

S000395042 not_calculated 0.937 1366 Pseudomonas koreensis (T); Ps 9-14; AF468452

S000428465 not_calculated 0.955 1426 Pseudomonas migulae (T); CIP 105470; AF074383

S000428886 not_calculated 0.953 1383 Pseudomonas brassicacearum (T); DBK11; CFBP 11706;

AF100321

> P48B-30

TGGCACGGGGGGAACGCGAAGAACCTTACCAGGCCTTGACATCCAATGAACTTTCTAGAGATAGATTGGTGCCTTC

GGGAACATTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAG

CGCAACCCTTGTCCTTAGTTACCAGCACGTAATGGTGGGCACTCTAAGGAGACTGCCGGTGACAAACCGGAGGAA

GGTGGGGATGACGTCAAGTCATCATGGCCCTTACGGCCTGGGCTACACACGTGCTACAATGGTCGGTACAGAGGG

TTGCCAAGCCGCGAGGTGGAGCTAATCCCACAAAACCGATCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGT

378 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

AY365101.1

Uncultured bacterium 17PS16S 16S ribosomal RNA

gene, partial sequence

675 675 99% 0.0 99%

AY365103.1

Uncultured bacterium 19PS16S 16S ribosomal RNA

gene, partial sequence

672 672 99% 0.0 99%

AY365102.1

Uncultured bacterium 18PS16S 16S ribosomal RNA

gene, partial sequence

672 672 99% 0.0 99%

Lineage:

Results for Query Sequence: P48B-30, 371 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 not_calculated 0.960 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 not_calculated 0.973 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 not_calculated 0.973 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366446 not_calculated 0.960 1414 Pseudomonas meridiana (T); type strain: CMS 38;

AJ537602

S000428331 not_calculated 0.973 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P48B-31

CAAGACCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTG

CAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGGTTGGCGGCGCTCTGTATGTAC

CATTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTC

ACCGGCAGTCTCATTAGAGTGCCCTTTCGTAGCAACTAATGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTACTGGTTCTCTTTCGAGCACTCCCTGATCTCTCA

381 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU536635.1

Uncultured bacterium clone nbt64c07 16S ribosomal

RNA gene, partial sequence

634 634 100% 1e-178

96%

EU536114.1

Uncultured bacterium clone nbt11g11 16S ribosomal

RNA gene, partial sequence

634 634 100% 1e-178

96%

EU536113.1

Uncultured bacterium clone nbt11g10 16S ribosomal

RNA gene, partial sequence

634 634 100% 1e-178

96%

Lineage:

Results for Query Sequence: P48B-31, 375 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Betaproteobacteria (5)

order Burkholderiales (5)

family Burkholderiaceae (1)

genus Limnobacter (1)

S000018694 - not_calculated 0.800 1426 Limnobacter thiooxidans (T); CS-K2; AJ289885

family Oxalobacteraceae (4)

genus Herbaspirillum (1)

S000002236 - not_calculated 0.805 1356 Herbaspirillum rubrisubalbicans (T); ATCC 19308T;

AB021424

genus Janthinobacterium (2)

S000006620 - not_calculated 0.840 1393 Janthinobacterium lividum (T); DSM 1522T; Y08846

S000008887 - not_calculated 0.813 1397 Janthinobacterium agaricidamnosum (T); W1r3T; Y08845

genus Herminiimonas (1)

S000592630 - not_calculated 0.821 1306 Herminiimonas aquatilis (T); type strain: CCUG 36956;

AM085762

> P48B-32

CAAGACCCGGGAACGTATCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGC

AGACTGCGATCTGGGCTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACC

ATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCA

CCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAA

CCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCT

CTAGAA

385 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU482922.1

Pseudomonas sp. CB19 16S ribosomal RNA gene,

partial sequence

679 679 100% 0.0 99%

EU538430.1

Uncultured bacterium clone nbt229e12 16S ribosomal

RNA gene, partial sequence

679 679 100% 0.0 99%

EU534578.1

Uncultured bacterium clone nbt181d03 16S ribosomal

RNA gene, partial sequence

679 679 100% 0.0 99%

Lineage:

Results for Query Sequence: P48B-32, 379 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.929 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.947 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.947 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.931 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.947 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P48B-33

GGCGGGGGCACGGGGGGAACGCGAAGAACCTTACCGGGCCTTGACATCCAATGAACTTTCTAGAGATAGATTGGT

GCCTTCGGGAACATTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGT

AACGAGCGCAACCCTTGTCCTTAGTTACCAGCACGTAATGGTGGGCACTCTAAGGAGACTGCCGGTGACAAACCG

GAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGGCCTGGGCTACACACGTGCTACAATGGTCGGTAC

AGAGGGTTGCCAAGCCGCGAGGTGGAGCTAATCCCACAAAACCGATCGTAGTCCGGATCGCAGTCTGCAACTCGA

CTGCGTGA

383 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

AY365101.1

Uncultured bacterium 17PS16S 16S ribosomal RNA

gene, partial sequence

682 682 100% 0.0 99%

AY365102.1

Uncultured bacterium 18PS16S 16S ribosomal RNA

gene, partial sequence

677 677 100% 0.0 99%

AY365099.1

Uncultured bacterium 15PS16S 16S ribosomal RNA

gene, partial sequence

677 677 100% 0.0 99%

Lineage:

Results for Query Sequence: P48B-33, 377 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 not_calculated 0.939 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 not_calculated 0.952 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 not_calculated 0.952 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366446 not_calculated 0.939 1414 Pseudomonas meridiana (T); type strain: CMS 38;

AJ537602

S000428331 not_calculated 0.952 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

>P48B-34

CAATACCCGGGAACGTATTCACCGCACCAATGCTGATCTGCGATTACTAGCGATTCCGACTTCATGGAGTCGAGTT

GCAGACTCCAATCCGGACTGAGATAGGGTTTTTGAGATTAGCATCCTATCGCTAGGTAGCAACCCTTTGTACCGAC

CATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCGTCCCCGCCTTCCTCCAGTTTGTC

ACTGGCAGTATCCTTAAAGTTCCCACCCGAAGTGCTGGCAAATAAGGAAAAGGGTTGCGCTCGTTGCGGGACTTAA

CCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTATGTAAGTTCCCGAAGGCACCAATCCATCT

CTGGAA

386 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

AM889158.1

Uncultured proteobacterium partial 16S rRNA gene,

clone 1'C12

643 643 98% 0.0 97%

FJ968638.1

Arcobacter sp. enrichment culture clone M4 16S

ribosomal RNA gene, partial sequence

643 643 98% 0.0 97%

FN432021.1

Uncultured bacterium partial 16S rRNA gene, isolate

w78

643 643 98% 0.0 97%

Lineage:

Results for Query Sequence: P48B-34, 374 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Moraxellaceae (5)

genus Acinetobacter (5)

S000001137 - not_calculated 0.869 1357 Acinetobacter johnsonii (T); DSM 6963; X81663

S000003452 - not_calculated 0.845 1366 Acinetobacter lwoffii (T); DSM 2403; X81665

S000008827 - not_calculated 0.869 1364 Acinetobacter haemolyticus (T); DSM 6962; X81662

S000127903 - not_calculated 0.885 1355 Acinetobacter ursingii (T); LUH 3792Type; AJ275038

S000381579 - not_calculated 0.850 1334 Acinetobacter calcoaceticus (T); ATCC 23055T; DNA

group one; Z93434

> P48B-35

GCGGGGCGGGGGCACCGGGGGAACGCGAATCACCTTACGGGGCCTTGGCATCCAATGAACTTTCTATCGATAGA

TTGGTGCCTTCGGGAACATTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGT

CCCGTAACGAGCGCAACCCTTGTCCTTAGTTACCAGCACGTAATGGTGGGCACTCTAAGGAGACTGCCGGTGACA

AACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGGCCTGGGCTACACACGTGCTACAATGGTC

GGTACAGAGGGTTGCCAAGCCGCGAGGTGGAGCTAATCCCAGAAAACCGATCGTAGTCCGGATCGCAGTCTGCAA

CTCGACTGCGTGA

387 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

AY365101.1

Uncultured bacterium 17PS16S 16S ribosomal RNA

gene, partial sequence

663 663 100% 0.0 97%

AY365095.1

Uncultured bacterium 11PS16S 16S ribosomal RNA

gene, partial sequence

657 657 100% 0.0 97%

AY365080.1

Pseudomonas sp. Ps6 16S ribosomal RNA gene,

partial sequence

657 657 100% 0.0 97%

Lineage:

Results for Query Sequence: P48B-35, 379 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000004663 not_calculated 0.871 1430 Pseudomonas veronii (T); CIP 104663; AF064460

S000013175 not_calculated 0.876 1405 Pseudomonas frederiksbergensis (T); JAJ28; AJ249382

S000013342 not_calculated 0.871 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145154 not_calculated 0.871 1434 Pseudomonas poae (T); type strain: DSM 14936 = LMG

21465; P 527/13; AJ492829

S000390380 not_calculated 0.889 1401 Pseudomonas grimontii (T); CFML 97-514T; AF268029

> P48B-36

CAATACCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCACTCGAGTT

GCAGAGTGCGATCCGGACTACGATCGGTTTTCTGGGATTGGCTCCACCTCGCGGCTTGGCGACCCTCTGTTCCGA

CCATTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGTTTGT

CACCGGCAGTCTCCCTGGAGTGCTCTTGCGTAGCAACTAGGGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCA

ACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTGATGGCTCCCTTTCGGGCACTCCCACCTCTC

AGCGG

382 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

FJ025135.1

Burkholderia cepacia strain SEMIA 6422 16S

ribosomal RNA gene, partial sequence

679 679 98% 0.0 100%

FJ025134.1

Burkholderia cepacia strain SEMIA 6417 16S

ribosomal RNA gene, partial sequence

679 679 98% 0.0 100%

AB299594.1

Burkholderia sp. M38-VN3-2W gene for 16S rRNA,

partial sequence

679 679 98% 0.0 100%

Lineage:

Results for Query Sequence: P48B-36, 373 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Betaproteobacteria (5)

order Burkholderiales (5)

family Burkholderiaceae (5)

genus Burkholderia (5)

S000008217 - not_calculated 0.949 1505 Burkholderia graminis (T); C4D1M (type strain); U96939

S000389259 - not_calculated 0.917 1382 Burkholderia caledonica (T); LMG 19076; AF215704

S000389260 - not_calculated 0.909 1380 Burkholderia fungorum (T); LMG 16225; AF215705

S000434814 - not_calculated 0.946 1408 Burkholderia terricola (T); LMG 20594T; AY040362

S000532739 - not_calculated 0.984 1402 Burkholderia phenoliruptrix (T); AC1100; AY435213

> P48B-37

CAAGACCCGGGAACGTATCACCGCCGCAAAGCTGATCTGCGATTACTAGCGATTCCGACTTCATGGAGTCGAGTT

GCAGACTCCAATCCGGACTGAGATAGGGTTTCTGGGATTGGCTTACCGTCGCCGGCTTGCAGCCCTCTGTCCCTA

CCATTGTAGTACGTGTGTAGCCCTGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGT

CACCGGCGGTCTCCTTAGAGTTCCCACCATTACGTGCTGGCAACTAAGGACAAGGGTTGCGCTCGTTGCGGGACT

TAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTCGAGTTCCCGAGGGCACCAATCC

ATCTCTGGAA

386 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GQ894723.1

Stenotrophomonas maltophilia strain NKY-3 16S

ribosomal RNA gene, partial sequence

677 677 100% 0.0 98%

FJ976542.1

Stenotrophomonas sp. LCR33 16S ribosomal RNA

gene, partial sequence

677 677 100% 0.0 98%

EF033231.1

Uncultured bacterium clone JH-YT40 16S ribosomal

RNA gene, partial sequence

677 677 100% 0.0 98%

Lineage:

Results for Query Sequence: P48B-37, 376 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Xanthomonadales (5)

family Xanthomonadaceae (5)

genus Stenotrophomonas (5)

S000000936 - not_calculated 0.912 1382 Pseudomonas pictorum (T); ATCC 23328T; AB021392

S000007629 - not_calculated 0.923 1345 Pseudomonas geniculata (T); ATCC 19374T; AB021404

S000010261 - not_calculated 0.907 1400 Pseudomonas hibiscicola (T); ATCC 19867T; AB021405

S000381870 - not_calculated 0.923 1384 Stenotrophomonas maltophilia (T); ATCC 13637T;

AB008509

S000390459 - not_calculated 0.888 1434 Stenotrophomonas acidaminiphila (T); AMX19; AF273080

> P48B-38

CAAGACCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTG

CAGACTGCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGAC

CATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTC

ACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTA

ACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATC

TCTAGAA

386 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU482922.1

Pseudomonas sp. CB19 16S ribosomal RNA gene,

partial sequence

697 697 100% 0.0 100%

DQ480483.1

Uncultured bacterium clone G24 16S ribosomal RNA

gene, partial sequence

697 697 100% 0.0 100%

DQ469202.1

Uncultured bacterium clone SX2-3 16S ribosomal

RNA gene, partial sequence

697 697 100% 0.0 100%

Lineage:

Results for Query Sequence: P48B-38, 380 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.968 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.987 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.987 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.971 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.987 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P48B-39

CAAGACCCGGGAACGTATTCACCGTCACATTCTGATTCACGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTG

CAGACTGCGATCCGGACTACGATCGGCTTTATGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTTTGTACCGAC

CATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTATC

ACCGGCAGTCTCCTTTGAGTTCCCGCCATTACGCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTT

AACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAGAGTTCCCGAAGGCACCAAAGCAT

CTCTGCTA

386 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

FJ515755.1

Uncultured bacterium clone 8 16S ribosomal RNA

gene, partial sequence

641 641 99% 0.0 96%

EU014836.1

Uncultured bacterium clone 3-5 16S ribosomal RNA

gene, partial sequence

634 634 100% 1e-178

96%

CU915097.1

Uncultured bacterium, partial 16S rRNA gene 623 623 99% 2e-175

96%

Lineage:

Results for Query Sequence: P48B-39, 376 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000008929 - not_calculated 0.771 1433 Pseudomonas taetrolens (T); IAM1653; D84027

S000014863 - not_calculated 0.777 1375 Pseudomonas lundensis (T); ATCC 49968T; AB021395

S000428774 - not_calculated 0.771 1373 Pseudomonas fragi (T); ATCC 4973; AF094733

S000437391 - not_calculated 0.763 1366 Pseudomonas stutzeri (T); CCUG 11256; U26262

S000540395 - not_calculated 0.769 1403 Pseudomonas stutzeri (T); AW-1; AY017341

>P43-25 1

CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACT

GCGATCCGAACTGAGAACAGATTTATGGGATTGGCTAAACCTTGCGGTCTCGCAGCCCTTTGTTCTGTCCATTGTA

GCACGTGTGTAGCCCAGGTCATAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTGACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

401 bases

Accession Description

Max

score

Total

score

Query

coverage

E value

Max

ident

DQ001315.1

Pseudomonas sp. SC11 16S ribosomal RNA

gene, partial sequence

616 616 99% 3e-173 94%

FJ820251.1

Uncultured bacterium isolate DGGE gel band

18 16S ribosomal RNA gene, partial sequence

612 612 99% 4e-172 94%

FJ937929.1

Pseudomonas sp. LS227 16S ribosomal RNA

gene, partial sequence

601 601 100% 7e-169 93%

Lineage:

Results for Query Sequence: P43-25, 395 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000002852 - not_calculated 0.744 1436 Pseudomonas psychrophila (T); E-3; AB041885

S000013342 - not_calculated 0.747 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145154 - not_calculated 0.747 1434 Pseudomonas poae (T); type strain: DSM 14936 = LMG

21465; P 527/13; AJ492829

S000428774 - not_calculated 0.744 1373 Pseudomonas fragi (T); ATCC 4973; AF094733

S000499371 - not_calculated 0.747 1399 Pseudomonas costantinii (T); CFBP 5705; HAMBI 2444;

AF374472

> P43-25 2

CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

726 726 100% 0.0 100%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

726 726 100% 0.0 100%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

726 726 100% 0.0 100%

GU198115.1

Pseudomonas fluorescens strain LMG 7207 16S

ribosomal RNA gene, partial sequence

726 726 100% 0.0 100%

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.982 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 1.000 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 1.000 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.985 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 1.000 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-25 3

CGTGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGACTGGTTTTATGGGATTAGCTCCCCCTCGCGGGTTGGCAACCCTCTGTACCAGCCATTGTAT

GACGTGTGTAGCCCCACCTATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCATTAGAGTGCCCAACTAAATGTAGCAACTAATGGCAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATC

TCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTACGGCTCTCTTTCGAGCACTCCTCTATCTCTAAAGG

ATTCCGTACATGTCAAAGGTGG

401 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

FM204986.1

Uncultured beta proteobacterium partial 16S rRNA

gene, clone B-UP-T0_OTU6

697 697 100% 0.0 98%

CU921194.1

Uncultured Betaproteobacteria bacterium 16S rRNA

gene from clone QEDS1CC12

697 697 100% 0.0 98%

EU521706.1

Acidovorax sp. NO-1 16S ribosomal RNA gene, partial

sequence

697 697 100% 0.0 98%

100

Lineage:

Results for Query Sequence: P43-25, 390 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Betaproteobacteria (5)

order Burkholderiales (5)

family Comamonadaceae (4)

genus Acidovorax (4)

S000009495 - not_calculated 0.874 1319 Acidovorax avenae subsp. avenae (T); ATCC 19307T;

AB021421

S000428517 - not_calculated 0.879 1424 Acidovorax citrulli (T); ATCC 29625; AF078761

S000428521 - not_calculated 0.910 1420 Acidovorax facilis (T); CCUG 2113; AF078765

S000428522 - not_calculated 0.915 1413 Acidovorax temperans (T); CCUG 11779; AF078766

family Incertae sedis 5 (1)

genus Xylophilus (1)

S000428514 - not_calculated 0.910 1434 Xylophilus ampelinus (T); ATCC 33914; AF078758

> P43-25 4

CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTATGGGGTTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198119.1

Pseudomonas fluorescens strain LMG 14571 16S

ribosomal RNA gene, partial sequence

720 720 100% 0.0 99%

GU198118.1

Pseudomonas fluorescens strain LMG 14566 16S

ribosomal RNA gene, partial sequence

720 720 100% 0.0 99%

GU198117.1

Pseudomonas fluorescens strain LMG 14562 16S

ribosomal RNA gene, partial sequence

720 720 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.985 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145154 - not_calculated 0.985 1434 Pseudomonas poae (T); type strain: DSM 14936 = LMG

21465; P 527/13; AJ492829

S000145156 - not_calculated 0.972 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.972 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000390380 - not_calculated 0.972 1401 Pseudomonas grimontii (T); CFML 97-514T; AF268029

101

> P43-25 5

CGGGAACGTACTCACCGCGGCATTCTGATCCGCGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTC

CAATCCGGACTACGATCGGCTTTTTGAGGTTAGCATCCTATCGCTAGGTAGCAACCCTTTGTACCGACCATTGTAG

CACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCGTCCCCGCCTTCCTCCAGTTTGTCACTGGCA

GTATCCTTAAAGTTCCCACCCGAAGTGCTGGCAAATAAGGAAAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTATGTAGGTTCCCGAAGGCACCAATCCATCTCTGGAAA

GTTCCTACTATGTCAAGACCAG

401 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GQ009281.1

Uncultured bacterium clone nbw829e04c1 16S

ribosomal RNA gene, partial sequence

710 710 100% 0.0 99%

AM889158.1

Uncultured proteobacterium partial 16S rRNA gene,

clone 1'C12

706 706 100% 0.0 99%

FJ968638.1

Arcobacter sp. enrichment culture clone M4 16S

ribosomal RNA gene, partial sequence

706 706 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-25, 391 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Moraxellaceae (5)

genus Acinetobacter (5)

S000001137 - not_calculated 0.910 1357 Acinetobacter johnsonii (T); DSM 6963; X81663

S000003452 - not_calculated 0.890 1366 Acinetobacter lwoffii (T); DSM 2403; X81665

S000008827 - not_calculated 0.910 1364 Acinetobacter haemolyticus (T); DSM 6962; X81662

S000127903 - not_calculated 0.926 1355 Acinetobacter ursingii (T); LUH 3792Type; AJ275038

S000381579 - not_calculated 0.877 1334 Acinetobacter calcoaceticus (T); ATCC 23055T; DNA

group one; Z93434

> P43-25 6

CGGGAACGTATTCACCGCCACATTCTGATTCTCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU539661.1

Uncultured bacterium clone nbt243g02 16S ribosomal

RNA gene, partial sequence

720 720 100% 0.0 99%

EU539660.1

Uncultured bacterium clone nbt243f12 16S ribosomal

RNA gene, partial sequence

720 720 100% 0.0 99%

EU539403.1

Uncultured bacterium clone nbt238c08 16S ribosomal

RNA gene, partial sequence

720 720 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.947 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.965 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.965 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.949 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.965 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

102

> P43-25 7

CGGGAACGTGATCACCGCCACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAGGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGTACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU539469.1

Uncultured bacterium clone nbt103g04 16S ribosomal

RNA gene, partial sequence

708 708 100% 0.0 99%

EU537964.1

Uncultured bacterium clone nbt228b07 16S ribosomal

RNA gene, partial sequence

708 708 100% 0.0 99%

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

702 702 100% 0.0 98%

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.912 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.929 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.929 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.914 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.929 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-25 8

CGGGAACGTCTTCATCGCCACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGGTTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTA

GCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGC

AGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAA

CATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGA

AAGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

FJ894027.1

Uncultured bacterium clone nbt175b10 16S ribosomal

RNA gene, partial sequence

711 711 100% 0.0 99%

EU482923.1

Pseudomonas sp. CB20 16S ribosomal RNA gene,

partial sequence

711 711 100% 0.0 99%

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

708 708 100% 0.0 99%

103

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.934 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145154 - not_calculated 0.934 1434 Pseudomonas poae (T); type strain: DSM 14936 = LMG

21465; P 527/13; AJ492829

S000145156 - not_calculated 0.947 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.947 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000428331 - not_calculated 0.947 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-25 9

CGGGCACGTCTTCACCGCCCCATTCTGCTTCTCGCTTACTAGCGCTTCCGACTTCACGCAGTCGAGTTGCATCCTG

CGCTCCGGACTACTCTCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

FJ893749.1

Uncultured bacterium clone nbt24f06 16S ribosomal

RNA gene, partial sequence

672 672 100% 0.0 97%

EU539661.1

Uncultured bacterium clone nbt243g02 16S ribosomal

RNA gene, partial sequence

672 672 100% 0.0 97%

EU539660.1

Uncultured bacterium clone nbt243f12 16S ribosomal

RNA gene, partial sequence

672 672 100% 0.0 97%

Lineage:

Results for Query Sequence: P43-25, 395 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000022197 - not_calculated 0.841 1381 Pseudomonas fluorescens (T); DSM 50090T (type strain);

Z76662

S000145156 - not_calculated 0.856 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.856 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.841 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.856 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

104

> P43-25 10

CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTATGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATTTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCCATCTCTGGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

AB506067.1

Pseudomonas sp. StFRB218 gene for 16S rRNA,

partial sequence

720 720 100% 0.0 99%

GU086437.1

Pseudomonas sp. M3 16S ribosomal RNA gene,

partial sequence

720 720 100% 0.0 99%

GQ994885.1

Uncultured organism clone Jwat28 16S ribosomal

RNA gene, partial sequence

720 720 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000004663 - not_calculated 0.970 1430 Pseudomonas veronii (T); CIP 104663; AF064460

S000013175 - not_calculated 0.970 1405 Pseudomonas frederiksbergensis (T); JAJ28; AJ249382

S000395040 - not_calculated 0.987 1369 Pseudomonas umsongensis (T); Ps 3-10; AF468450

S000428381 - not_calculated 0.987 1398 Pseudomonas jessenii (T); CIP 105274; AF068259

S000428763 - not_calculated 0.972 1363 Pseudomonas chlororaphis subsp. aureofaciens (T);

ATCC 13985; AF094722

>P43-25 11

CGGGAACGTATTCACCGTGACATTCTGATTCACGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTATGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTTTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCCATCTCTGGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198122.1

Pseudomonas fluorescens strain LMG 14577 16S

ribosomal RNA gene, partial sequence

726 726 100% 0.0 100%

GU124695.1

Pseudomonas sp. CT9 16S ribosomal RNA gene,

partial sequence

726 726 100% 0.0 100%

GU124694.1

Pseudomonas sp. CT3 (2009) 16S ribosomal RNA

gene, partial sequence

726 726 100% 0.0 100%

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000002852 - not_calculated 0.952 1436 Pseudomonas psychrophila (T); E-3; AB041885

S000008929 - not_calculated 1.000 1433 Pseudomonas taetrolens (T); IAM1653; D84027

S000014863 - not_calculated 1.000 1375 Pseudomonas lundensis (T); ATCC 49968T; AB021395

S000395040 - not_calculated 0.949 1369 Pseudomonas umsongensis (T); Ps 3-10; AF468450

S000428774 - not_calculated 0.965 1373 Pseudomonas fragi (T); ATCC 4973; AF094733

105

>P43-25 12

CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAAGTTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

FJ893918.1

Uncultured bacterium clone nbt123h07 16S ribosomal

RNA gene, partial sequence

717 717 100% 0.0 99%

FJ893273.1

Uncultured bacterium clone nbt19c04 16S ribosomal

RNA gene, partial sequence

717 717 100% 0.0 99%

EU539081.1

Uncultured bacterium clone nbt98a12 16S ribosomal

RNA gene, partial sequence

717 717 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.960 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.977 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.977 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.962 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.977 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

>P43-25 13

CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

726 726 100% 0.0 100%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

726 726 100% 0.0 100%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

726 726 100% 0.0 100%

106

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.982 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 1.000 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 1.000 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.985 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 1.000 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

>P43-25 14

CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACT

GCGATCCGGACTACGACTGGTTTTATGGGATTAGCTCCCCCTCGCGGGTGGCGGCGCTCTGTATGTACCATTGTAT

GACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCATTAGAGTGCCCTTTCGTAGCAACTAATGACAAGGGTTGCGCTCGTTGCGGGACTTATCCCAACATCTCAC

GACACGAGCCGACGACAGCCATGCAGCACCTGTGTACTGGTTCTCTTTCGAGCACTCCCCAATCTCTCGGGGATT

CCAGCCATGTCAAGGGTAG

396 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU213421.1

Janthinobacterium sp. 286 16S ribosomal RNA gene,

partial sequence

645 645 100% 0.0 96%

FJ225384.1

Uncultured Janthinobacterium sp. clone KunzBra2 16S

ribosomal RNA gene, partial sequence

645 645 100% 0.0 96%

GQ451214.1

Uncultured bacterium clone J72 16S ribosomal RNA

gene, partial sequence

645 645 100% 0.0 96%

GQ398374.1

Bacterium B7(2009) 16S ribosomal RNA gene, partial

sequence

645 645 100% 0.0 96%

Lineage:

Results for Query Sequence: P43-25, 386 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Betaproteobacteria (5)

order Burkholderiales (5)

family Oxalobacteraceae (5)

genus Herbaspirillum (3)

S000006848 - not_calculated 0.795 1408 Herbaspirillum huttiense (T); ATCC 14670T; AB021366

S000014269 - not_calculated 0.795 1442 Herbaspirillum seropedicae (T); DSM 6445, ATCC 35892;

Y10146

S000439511 - not_calculated 0.795 1418 Herbaspirillum putei (T); 7-2; AB109890

genus Janthinobacterium (2)

S000006620 - not_calculated 0.870 1393 Janthinobacterium lividum (T); DSM 1522T; Y08846

S000008887 - not_calculated 0.806 1397 Janthinobacterium agaricidamnosum (T); W1r3T; Y08845

107

>P43-25 15

CGGGAACGTATTCACCGCGACATTCCGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAATGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTACTGGTTCTCTTTCGAGCACTCCCCAATCTCTCG

GGGATTCCAGCCATGTCAAGGGTAG

404 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU538064.1

Uncultured bacterium clone nbt81e05 16S ribosomal

RNA gene, partial sequence

706 706 100% 0.0 98%

EU540348.1

Uncultured bacterium clone nbt113f07 16S ribosomal

RNA gene, partial sequence

702 702 100% 0.0 98%

GQ416410.1

Uncultured Alcaligenes sp. clone F7may1.21 16S

ribosomal RNA gene, partial sequence

675 675 100% 0.0 97%

Lineage:

Results for Query Sequence: P43-25, 394 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000145156 - not_calculated 0.835 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.832 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000395042 - not_calculated 0.832 1366 Pseudomonas koreensis (T); Ps 9-14; AF468452

S000428331 - not_calculated 0.835 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

S000428332 - not_calculated 0.832 1422 Pseudomonas cedrina (T); CFML 96-198; AF064461

>P43-25 16

CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

726 726 100% 0.0 100%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

726 726 100% 0.0 100%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

726 726 100% 0.0 100%

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.982 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 1.000 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 1.000 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.985 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 1.000 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

108

>P43-25 17

TTGACATGGCTGGAATCCTCGAGAGATCGGGGAGTGCTCGAAAGAGAACCAGTACACAGGTGCTGCATGGCTGTC

GTCAGCTCGTGTCGTGAGACGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTGTCCTTAGTTACCAGCACGTAAT

GGTGGGCACTCTAAGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTT

ATGGGTAGGGCTTCACACGTCATACAATGGTACATACAGAGCGCCGCCAACCCGCGAGGGGGAGCTAATCCCATA

AAACCGATCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGTGAAGTCGGAATCGCTAGTAATCGTGAATCAGAA

TGTCACGGTGAATACGTTCCCGG

399 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU535428.1

Uncultured bacterium clone nbt02g06 16S ribosomal

RNA gene, partial sequence

652 652 100% 0.0 96%

EU535408.1

Uncultured bacterium clone nbt02e10 16S ribosomal

RNA gene, partial sequence

639 639 100% 3e-180

95%

EU540348.1

Uncultured bacterium clone nbt113f07 16S ribosomal

RNA gene, partial sequence

625 625 100% 7e-176

94%

Lineage:

Results for Query Sequence: P43-25, 391 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Betaproteobacteria (1)

order Burkholderiales (1)

family Oxalobacteraceae (1)

genus Janthinobacterium (1)

S000006620 not_calculated 0.719 1393 Janthinobacterium lividum (T); DSM 1522T; Y08846

class Gammaproteobacteria (4)

order Pseudomonadales (4)

family Pseudomonadaceae (4)

genus Pseudomonas (4)

S000004100 not_calculated 0.703 1397 Pseudomonas pseudoalcaligenes (T); DSM 50018T (type

strain); Z76675

S000014863 not_calculated 0.701 1375 Pseudomonas lundensis (T); ATCC 49968T; AB021395

S000428774 not_calculated 0.701 1373 Pseudomonas fragi (T); ATCC 4973; AF094733

S000626937 not_calculated 0.701 1426 Pseudomonas nitroreducens (T); type strain:DSM 14399;

AM088474

>P43-25 18

CGGGAACGTATTCCCGCGCCATTCTGATTCGTGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAAGGCCTG

401 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU538653.1

Uncultured bacterium clone nbt234e10 16S ribosomal

RNA gene, partial sequence

713 713 100% 0.0 99%

EU538625.1

Uncultured bacterium clone nbt234b05 16S ribosomal

RNA gene, partial sequence

713 713 100% 0.0 99%

EU538498.1

Uncultured bacterium clone nbt230g07 16S ribosomal

RNA gene, partial sequence

713 713 100% 0.0 99%

109

Lineage:

Results for Query Sequence: P43-25, 395 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.937 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.954 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.954 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.939 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.954 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

>P43-25 19

CGGGTCCGTCTTCACCGCCCCATTCTGCTTCGCTCTTACTAGCGCTTCCGCCTTCACGCAGTCGAGTTGCATCCTG

CGTCTCCGGCCTACTCTAGGTTTTGTGGGCTTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTA

GCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGC

AGTCTCCTTAGAGTGCCCACCATGACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAA

CATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGA

AAGTTCATTGGATGTCAAGGCCTG

403 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

FJ894580.1

Uncultured bacterium clone nbt182h10 16S ribosomal

RNA gene, partial sequence

643 643 100% 0.0 95%

FJ894133.1

Uncultured bacterium clone nbt176e10 16S ribosomal

RNA gene, partial sequence

643 643 100% 0.0 95%

EU540186.1

Uncultured bacterium clone nbt246g02 16S ribosomal

RNA gene, partial sequence

643 643 100% 0.0 95%

Lineage:

Results for Query Sequence: P43-25, 397 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000145156 - not_calculated 0.793 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.793 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.793 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428195 - not_calculated 0.798 1422 Pseudomonas libanensis (T); CIP 105460; AF057645

S000428331 - not_calculated 0.791 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

110

>P43-25 20

CGGGAACGTACTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTCTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATAACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCA

338 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198105.1

Pseudomonas fluorescens strain LMG 5168 16S

ribosomal RNA gene, partial sequence

605 605 100%

5e-

170

99%

FJ375458.1

Uncultured bacterium clone ACS45 16S ribosomal

RNA gene, partial sequence

605 605 100%

5e-

170

99%

GQ867227.1

Pseudomonas sp. BRAZ13 16S ribosomal RNA gene,

partial sequence

605 605 100%

5e-

170

99%

Lineage:

Results for Query Sequence: P43-25, 332 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000004663 - not_calculated 0.967 1430 Pseudomonas veronii (T); CIP 104663; AF064460

S000010434 - not_calculated 0.970 1360 Pseudomonas corrugata (T); ATCC29736; D84012

S000013175 - not_calculated 0.967 1405 Pseudomonas frederiksbergensis (T); JAJ28; AJ249382

S000355057 - not_calculated 0.967 1299 Pseudomonas salomonii (T); CFBP 2022; AY091528

S000358323 - not_calculated 0.967 1425 Pseudomonas lutea (T); OK2; AY364537

> P43-25 21

CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACT

GCGATCCGAACTGAGAACAGATTTATGGGATTGGCTAAACCTTGCGGTCTCGCAGCCCTTTGTTCTGTCCATTGTA

GCACGTGTGTAGCCCAGGTCATAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTGACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

401 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

DQ001315.1

Pseudomonas sp. SC11 16S ribosomal RNA gene,

partial sequence

616 616 99%

3e-

173

94%

FJ820251.1

Uncultured bacterium isolate DGGE gel band 18 16S

ribosomal RNA gene, partial sequence

612 612 99%

4e-

172

94%

FJ937929.1

Pseudomonas sp. LS227 16S ribosomal RNA gene,

partial sequence

601 601 100%

7e-

169

93%

Lineage:

Results for Query Sequence: P43-25, 395 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000002852 - not_calculated 0.744 1436 Pseudomonas psychrophila (T); E-3; AB041885

S000013342 - not_calculated 0.747 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145154 - not_calculated 0.747 1434 Pseudomonas poae (T); type strain: DSM 14936 = LMG

21465; P 527/13; AJ492829

S000428774 - not_calculated 0.744 1373 Pseudomonas fragi (T); ATCC 4973; AF094733

S000499371 - not_calculated 0.747 1399 Pseudomonas costantinii (T); CFBP 5705; HAMBI 2444;

AF374472

111

> P43-25 22

CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCTATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

720 720 100% 0.0 99%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

720 720 100% 0.0 99%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

720 720 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.965 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.982 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.982 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.967 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.982 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-25 23

CGGGAACGTATTCACCGCCGCAATGCTGATCTGCGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACT

CCAATCCGGACTGAGATAGGGTTTCTGGGATTGGCTTACCGTCGCCGGCTTGCAGCCCTCTGTCCCTACCATTGTA

GTACGTGTGTAGCCCTGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGC

GGTCTCCTTAGAGTTCCCACCATTACGTGCTGGCAACTAAGGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAA

CATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTCGAGTACCCGAAGGCACCAATCCATCTCTGG

AAAGTTCTCGACATGTCAAGGCCAG

403 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU254009.1

Stenotrophomonas sp. 2057 16S ribosomal RNA gene,

partial sequence

719 719 100% 0.0 99%

GQ268318.1

Stenotrophomonas maltophilia strain Y1 16S ribosomal

RNA gene, partial sequence

719 719 100% 0.0 99%

GU060525.1

Stenotrophomonas maltophilia strain KT814 16S

ribosomal RNA gene, partial sequence

719 719 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Xanthomonadales (5)

family Xanthomonadaceae (5)

genus Stenotrophomonas (5)

S000000936 - not_calculated 0.942 1382 Pseudomonas pictorum (T); ATCC 23328T; AB021392

S000007629 - not_calculated 0.967 1345 Pseudomonas geniculata (T); ATCC 19374T; AB021404

S000010261 - not_calculated 0.952 1400 Pseudomonas hibiscicola (T); ATCC 19867T; AB021405

S000381870 - not_calculated 0.967 1384 Stenotrophomonas maltophilia (T); ATCC 13637T;

AB008509

S000390459 - not_calculated 0.912 1434 Stenotrophomonas acidaminiphila (T); AMX19; AF273080

112

> P43-25 24

CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

726 726 100% 0.0 100%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

726 726 100% 0.0 100%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

726 726 100% 0.0 100%

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.982 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 1.000 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 1.000 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.985 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 1.000 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-25 25

CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

726 726 100% 0.0 100%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

726 726 100% 0.0 100%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

726 726 100% 0.0 100%

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.982 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 1.000 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 1.000 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.985 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 1.000 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

113

> P43-25 26

CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACAAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

720 720 100% 0.0 99%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

720 720 100% 0.0 99%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

720 720 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.965 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.982 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.982 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.967 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.982 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

>P43-25 27

CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGATGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

720 720 100% 0.0 99%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

720 720 100% 0.0 99%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

720 720 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-25, 395 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.967 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.985 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.985 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.970 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.985 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

114

> P43-25 28

CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTAGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

720 720 100% 0.0 99%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

720 720 100% 0.0 99%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

720 720 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.965 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.982 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.982 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.967 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.982 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

>P43-25 29

CGGGAACGTATTCACCGCGCCATTCTGATTCGTGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU538653.1

Uncultured bacterium clone nbt234e10 16S ribosomal

RNA gene, partial sequence

720 720 100% 0.0 99%

EU538625.1

Uncultured bacterium clone nbt234b05 16S ribosomal

RNA gene, partial sequence

720 720 100% 0.0 99%

EU538498.1

Uncultured bacterium clone nbt230g07 16S ribosomal

RNA gene, partial sequence

720 720 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.947 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.965 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.965 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.949 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.965 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

115

> P43-25 30

CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTCTGGGATTAGCTCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATAACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAAGGCCTG

401 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

FJ375458.1

Uncultured bacterium clone ACS45 16S ribosomal

RNA gene, partial sequence

717 717 100% 0.0 99%

GQ847629.1

Uncultured bacterium isolate DGGE gel band BAC7

16S ribosomal RNA gene, partial sequence

717 717 100% 0.0 99%

GQ847628.1

Uncultured bacterium isolate DGGE gel band BAC6

16S ribosomal RNA gene, partial sequence

717 717 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-25, 395 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000355057 - not_calculated 0.957 1299 Pseudomonas salomonii (T); CFBP 2022; AY091528

S000366447 - not_calculated 0.970 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000390380 - not_calculated 0.970 1401 Pseudomonas grimontii (T); CFML 97-514T; AF268029

S000428195 - not_calculated 0.970 1422 Pseudomonas libanensis (T); CIP 105460; AF057645

S000428466 - not_calculated 0.970 1415 Pseudomonas gessardii (T); CIP 105469; AF074384

> P43-25 31

CGGGAACGTGTTCACCGCGCCATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTCTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATAACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

TGTTCATTGGATGTCAAGGCCTG

402 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

FJ375458.1

Uncultured bacterium clone ACS45 16S ribosomal

RNA gene, partial sequence

711 711 100% 0.0 99%

GQ847629.1

Uncultured bacterium isolate DGGE gel band BAC7

16S ribosomal RNA gene, partial sequence

711 711 100% 0.0 99%

GQ847628.1

Uncultured bacterium isolate DGGE gel band BAC6

16S ribosomal RNA gene, partial sequence

711 711 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-25, 395 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000355057 - not_calculated 0.919 1299 Pseudomonas salomonii (T); CFBP 2022; AY091528

S000366447 - not_calculated 0.932 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000390380 - not_calculated 0.932 1401 Pseudomonas grimontii (T); CFML 97-514T; AF268029

S000428195 - not_calculated 0.932 1422 Pseudomonas libanensis (T); CIP 105460; AF057645

S000428466 - not_calculated 0.932 1415 Pseudomonas gessardii (T); CIP 105469; AF074384

116

> P43-25 32

CGGGAACGTCTTCACCGCTCCATTCTGATTCGCTCTTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCAGTTTTGTGGGATTATCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCATGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACAGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACTAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGACTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU538653.1

Uncultured bacterium clone nbt234e10 16S ribosomal

RNA gene, partial sequence

720 720 100% 0.0 99%

EU538625.1

Uncultured bacterium clone nbt234b05 16S ribosomal

RNA gene, partial sequence

720 720 100% 0.0 99%

EU538498.1

Uncultured bacterium clone nbt230g07 16S ribosomal

RNA gene, partial sequence

720 720 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.846 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000022197 - not_calculated 0.851 1381 Pseudomonas fluorescens (T); DSM 50090T (type strain);

Z76662

S000145156 - not_calculated 0.861 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.861 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000428331 - not_calculated 0.861 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-25 33

CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTATGTAAGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

702 702 100% 0.0 98%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

702 702 100% 0.0 98%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

702 702 100% 0.0 98%

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.937 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.955 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.955 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.939 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.955 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

117

> P43-25 34

CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGGTCCAACTTCATGCAGTCGAGTTGCAGACTA

CAATCCGGACTACGATACACTTTCTGCGATTAGCTCCCCCTCGCGGGTTGGCGGCGCTCTGTATGTACCATTGTAT

GACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCATTAGAGTGCCCTTTCGTAGCAACTAATGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCAC

GACACGAGCTGACGACAGCCATGCAGCACCTGTGTACTGGTTCTCTTTCGAGCACTCCTCAATCTCTCGAGGATTC

CAGCCATGTCAAGGGTAG

396 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

FJ893011.1

Uncultured bacterium clone nbt16d03 16S ribosomal

RNA gene, partial sequence

704 704 100% 0.0 99%

GQ179708.1

Uncultured Janthinobacterium sp. clone VE21B06 16S

ribosomal RNA gene, partial sequence

699 699 100% 0.0 99%

GQ101992.1

Uncultured bacterium clone nbw502b12c1 16S

ribosomal RNA gene, partial sequence

699 699 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-25, 385 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Betaproteobacteria (5)

order Burkholderiales (5)

family Oxalobacteraceae (5)

genus Duganella (1)

S000022017 - not_calculated 0.886 1375 Duganella zoogloeoides (T); IAM12670; D14256

genus Herbaspirillum (1)

S000002236 - not_calculated 0.849 1356 Herbaspirillum rubrisubalbicans (T); ATCC 19308T;

AB021424

genus Janthinobacterium (2)

S000006620 - not_calculated 0.943 1393 Janthinobacterium lividum (T); DSM 1522T; Y08846

S000008887 - not_calculated 0.896 1397 Janthinobacterium agaricidamnosum (T); W1r3T; Y08845

genus Herminiimonas (1)

S000574631 - not_calculated 0.849 1342 Herminiimonas fonticola (T); S-94; AY676462

> P43-25 35

CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGGTGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU537818.1

Uncultured bacterium clone nbt80c03 16S ribosomal

RNA gene, partial sequence

726 726 100% 0.0 100%

EU536570.1

Uncultured bacterium clone nbt63a07 16S ribosomal

RNA gene, partial sequence

726 726 100% 0.0 100%

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

720 720 100% 0.0 99%

118

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.965 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.982 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.982 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.967 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.982 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-25 36

CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCT

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

720 720 100% 0.0 99%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

720 720 100% 0.0 99%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

720 720 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-25, 395 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.967 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.985 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.985 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.970 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.985 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

119

> P43-25 37

CGGGAACGTATCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAAGGCCTG

401 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU630427.1

Uncultured Pseudomonas sp. clone URA1Q1u38_T3

16S ribosomal RNA gene, partial sequence

719 719 100% 0.0 99%

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

717 717 100% 0.0 99%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

717 717 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-25, 395 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.970 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.987 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.987 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.972 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.987 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-25 38

CGGGAACGTATTCACCCCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCTCATCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGAGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

708 708 100% 0.0 99%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

708 708 100% 0.0 99%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

708 708 100% 0.0 99%

120

Lineage:

Results for Query Sequence: P43-25, 395 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.932 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.949 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.949 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.934 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.949 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-25 39

CGGGAACGTTTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGGCAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCCATCTCTAGAAA

GTTCATTGGATGTCAAGGCCTG

401 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

AB506069.1

Pseudomonas sp. StFRB234 gene for 16S rRNA,

partial sequence

708 708 100% 0.0 99%

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

708 708 100% 0.0 99%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

708 708 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-25, 394 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000108891 - not_calculated 0.954 1409 Pseudomonas kilonensis (T); 520-20; AJ292426

S000145156 - not_calculated 0.957 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.957 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000428331 - not_calculated 0.957 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

S000428332 - not_calculated 0.957 1422 Pseudomonas cedrina (T); CFML 96-198; AF064461

121

> P43-25 40

CGGGAACGTATTCACCGTGACATTCTGATTCACGACTACTAGCAATTCCGACTTCACGCAGTCGAGTTGCAAACTG

CGATCCGGACTACGATCGGTTTTATGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTTTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTAGACGTCATCCCCACCTTCCTCCGGTTTGTCATCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCCATCTCTGGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GQ069182.1

Uncultured bacterium clone nbw229h11c1 16S

ribosomal RNA gene, partial sequence

708 708 100% 0.0 99%

EU336940.2

Pseudomonas sp. CT364 16S ribosomal RNA gene,

partial sequence

708 708 100% 0.0 99%

GU198122.1

Pseudomonas fluorescens strain LMG 14577 16S

ribosomal RNA gene, partial sequence

702 702 100% 0.0 98%

Lineage:

Results for Query Sequence: P43-25, 395 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000008929 - not_calculated 0.916 1433 Pseudomonas taetrolens (T); IAM1653; D84027

S000014863 - not_calculated 0.916 1375 Pseudomonas lundensis (T); ATCC 49968T; AB021395

S000395040 - not_calculated 0.873 1369 Pseudomonas umsongensis (T); Ps 3-10; AF468450

S000428381 - not_calculated 0.876 1398 Pseudomonas jessenii (T); CIP 105274; AF068259

S000428774 - not_calculated 0.881 1373 Pseudomonas fragi (T); ATCC 4973; AF094733

> P43-25 41

CGGGAACGTATTCACCGGCGCATGCTGATCTGCGATTACTAGCGATTCCGCCTTCATGCAGGCGAGTTGCAGCCT

GCAATCCGAACTGAGAGCTTGTTTTTGGGTTTGGCTCCACCTCGCGGCTTCGCTACCCTCTATTAAAGCCCATTGTA

GTACGTGTGTAGCCCAAGACATAAGGGGCATGATGACTTGACGTCATCCCCACCTTCCTCCGTGTTATCCACGGCA

GTCTCCCATGAGTTCCCACCATTACGTGCTGGCAACATAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTCTATCTGTCTTCCGAAGAAGAACTTCCTATCTCTAG

GAGCATCAGACGATGTCAAGCCTTG

405 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

AB486287.1

Uncultured bacterium gene for 16S ribosomal RNA,

partial sequence, clone: TSBAR002_E13

713 713 100% 0.0 99%

AB240341.1

Uncultured bacterium gene for 16S rRNA, partial

sequence, clone: RB334

708 708 100% 0.0 98%

EU215386.1

Pelosinus sp. UFO1 16S ribosomal RNA pseudogene,

complete sequence

704 704 100% 0.0 98%

DQ295866.1

Pelosinus sp. UFO1 16S ribosomal RNA gene, partial

sequence

704 704 100% 0.0 98%

122

Lineage:

Results for Query Sequence: P43-25, 398 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Firmicutes (5)

class "Clostridia" (5)

order Clostridiales (5)

family Veillonellaceae (5)

genus Anaerosinus (1)

S000112717 - not_calculated 0.626 1417 Anaerosinus glycerini (T); DSM 5192(T); AJ010960

genus Dendrosporobacter (1)

S000436474 - not_calculated 0.656 1340 Dendrosporobacter quercicolus (T); M59110

genus Propionispira (1)

S000016925 - not_calculated 0.646 1398 Propionispira arboris (T); DSM 2179; Y18190

genus Propionispora (2)

S000008602 - not_calculated 0.616 1427 Propionispora vibrioides (T); FKBS1; AJ279802

S000351774 - not_calculated 0.618 1436 Propionispora hippei (T); type strain: KS; 5; AJ508927

> P43-25 42

CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTCTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAAGGCCTG

402 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198125.1

Pseudomonas fluorescens strain LMG 14675 16S

ribosomal RNA gene, partial sequence

726 726 100% 0.0 100%

GQ985445.1

Pseudomonas sp. L-31 16S ribosomal RNA gene,

partial sequence

726 726 100% 0.0 100%

GQ891854.1

Uncultured Pseudomonas sp. clone G97 16S

ribosomal RNA gene, partial sequence

726 726 100% 0.0 100%

Lineage:

Results for Query Sequence: P43-25, 396 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.982 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145154 - not_calculated 0.982 1434 Pseudomonas poae (T); type strain: DSM 14936 = LMG

21465; P 527/13; AJ492829

S000145156 - not_calculated 0.982 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000390380 - not_calculated 1.000 1401 Pseudomonas grimontii (T); CFML 97-514T; AF268029

S000428195 - not_calculated 0.985 1422 Pseudomonas libanensis (T); CIP 105460; AF057645

123

>P43-36 1

GGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACGCGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

FJ893460.1

Uncultured bacterium clone nbt22a11 16S ribosomal

RNA gene, partial sequence

713 713 100% 0.0 100%

EU539109.1

Uncultured bacterium clone nbt98d07 16S ribosomal

RNA gene, partial sequence

713 713 100% 0.0 100%

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

708 708 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.964 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.982 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.982 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.967 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.982 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-36 2

GGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

713 713 100% 0.0 100%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

713 713 100% 0.0 100%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

713 713 100% 0.0 100%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.982 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 1.000 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 1.000 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.985 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 1.000 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

124

> P43-36 3

GGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

713 713 100% 0.0 100%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

713 713 100% 0.0 100%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

713 713 100% 0.0 100%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.982 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 1.000 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 1.000 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.985 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 1.000 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-36 4

GGGAACGTATTCACCGCCACATTCTGATTCTCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU539661.1

Uncultured bacterium clone nbt243g02 16S ribosomal

RNA gene, partial sequence

708 708 100% 0.0 99%

EU539660.1

Uncultured bacterium clone nbt243f12 16S ribosomal

RNA gene, partial sequence

708 708 100% 0.0 99%

EU539403.1

Uncultured bacterium clone nbt238c08 16S ribosomal

RNA gene, partial sequence

708 708 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.946 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.964 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.964 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.949 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.964 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

125

> P43-36 5

GGGATCGTATTCACCGCAGCATTCTGATCTACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCC

AATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGC

ACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCACCCCACCTTCCTCCAGTTTATCACTGGCAGTC

TCCTTTGAGTTCCCGGCCGAACCGCTGGCAACAAAGGATAAGGGTTGCGCTC

280 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

AB460226.1

Uncultured bacterium gene for 16S rRNA, partial

sequence, clone: 4101O18

495 495 100%

8e-

137

99%

GQ451698.1

Enterobacter sp. AN2 16S ribosomal RNA gene,

partial sequence

489 489 100%

3e-

135

98%

GU186121.1

Pantoea agglomerans strain IHB B 1383 16S

ribosomal RNA gene, partial sequence

489 489 100%

3e-

135

98%

Lineage:

Results for Query Sequence: P43-36, 274 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Enterobacteriales (5)

family Enterobacteriaceae (5)

genus Buttiauxella (3)

S000016869 - not_calculated 0.909 1408 Buttiauxella brennerae (T); DSM 9396; AJ233401

S000017991 - not_calculated 0.909 1404 Buttiauxella gaviniae (T); DSM 9393; AJ233403

S000022751 - not_calculated 0.912 1406 Buttiauxella izardii (T); DSM 9397; AJ233404

genus Cedecea (1)

S000120182 - not_calculated 0.956 1305 Cedecea davisae (T); DSM 4568; AF493976

genus Enterobacter (1)

S000381739 - not_calculated 0.931 1329 Enterobacter asburiae (T); JCM6051; AB004744

> P43-36 6

GGGAACGTATTCACCGCGCCATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTATGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTAGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU538622.1

Uncultured bacterium clone nbt234a03 16S ribosomal

RNA gene, partial sequence

708 708 100% 0.0 99%

GU198119.1

Pseudomonas fluorescens strain LMG 14571 16S

ribosomal RNA gene, partial sequence

704 704 100% 0.0 99%

GU198118.1

Pseudomonas fluorescens strain LMG 14566 16S

ribosomal RNA gene, partial sequence

704 704 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000005898 - not_calculated 0.946 1429 Pseudomonas azotoformans (T); IAM1603; D84009

S000013342 - not_calculated 0.964 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145154 - not_calculated 0.964 1434 Pseudomonas poae (T); type strain: DSM 14936 = LMG

21465; P 527/13; AJ492829

S000145156 - not_calculated 0.946 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000499371 - not_calculated 0.949 1399 Pseudomonas costantinii (T); CFBP 5705; HAMBI 2444;

AF374472

126

> P43-36 7

GGGAACGTATTCCCGCCACATTCTGATTCTCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGCG

ATCCGGACTACGATCGGTTTTATGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGCA

CGTGTGTAGCCCAGGCCGTAAGGGCCCTGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGT

CTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACAT

CTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGCTCCCGAAGGCACCAATCTATCTCTAGAAAG

TTCATTGGATGTCAA

394 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GQ024506.1

Uncultured bacterium clone nbw123b06c1 16S

ribosomal RNA gene, partial sequence

691 691 100% 0.0 98%

GQ024504.1

Uncultured bacterium clone nbw123b03c1 16S

ribosomal RNA gene, partial sequence

691 691 100% 0.0 98%

EU539699.1

Uncultured bacterium clone nbt244d04 16S ribosomal

RNA gene, partial sequence

691 691 100% 0.0 98%

Lineage:

Results for Query Sequence: P43-36, 388 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000002852 - not_calculated 0.915 1436 Pseudomonas psychrophila (T); E-3; AB041885

S000013342 - not_calculated 0.925 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145154 - not_calculated 0.928 1434 Pseudomonas poae (T); type strain: DSM 14936 = LMG

21465; P 527/13; AJ492829

S000428774 - not_calculated 0.923 1373 Pseudomonas fragi (T); ATCC 4973; AF094733

S000499371 - not_calculated 0.912 1399 Pseudomonas costantinii (T); CFBP 5705; HAMBI 2444;

AF374472

> P43-36 8

GGGAACGTATTCACCGCCACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCACTATACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCATCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGAGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCTATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GQ077867.1

Uncultured bacterium clone nbw1212e08c1 16S

ribosomal RNA gene, partial sequence

690 690 100% 0.0 98%

EU539968.1

Uncultured bacterium clone nbt108g01 16S ribosomal

RNA gene, partial sequence

690 690 100% 0.0 98%

EU536044.1

Uncultured bacterium clone nbt07f10 16S ribosomal

RNA gene, partial sequence

690 690 100% 0.0 98%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.897 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.915 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.915 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.905 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.915 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

127

> P43-36 9

GGGAACGTATTCACCGCGACATGCTGATCCGCGATTACTAGCGATTCCAACTTCATGCAGTCGAGTTGCAGACTGC

GATCCGAACTGAGAACAGATTTATGGGATTGGCTAAACCTTGCGGTCTCGCAGCCCTTTGTTCTGTCCATTGTAGC

ACGTGTGCGGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCACCTTAGAGTGCCCAACTGAATGCTGGCAACCAAGATCAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATC

TCACGACACGAGCTGACGACAACCATGCACCACCTGTCACTCTGTCCCCGAAGGGAAAGCCCTATCTCTAGGGTT

GTCAGAGGATGTCAA

393 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU435356.1

Bacillus sp. M3-16 16S ribosomal RNA gene, partial

sequence

682 682 100% 0.0 98%

AB244484.1

Bacillus pumilus gene for 16S rRNA, partial

sequence, strain: limp 5-3

682 682 100% 0.0 98%

EU231626.1

Bacillus pumilus strain TCCC11014 16S ribosomal

RNA gene, partial sequence

682 682 100% 0.0 98%

Lineage:

Results for Query Sequence: P43-36, 385 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Firmicutes (5)

class "Bacilli" (5)

order Bacillales (5)

family Bacillaceae (5)

subfamily "Bacillaceae 1" (5)

supergenus Bacillus (5)

genus Bacillus a (5)

S000378387 - not_calculated 0.896 1399 Bacillus pumilus (T); DSMZ27; AY456263

S000417318 - not_calculated 0.896 1415 Bacillus altitudinis (T); type strain:41KF2b; AJ831842

S000458519 - not_calculated 0.899 1354 Bacillus safensis (T); FO-036b; AF234854

S001014161 - not_calculated 0.896 1443 Bacillus stratosphericus (T); type strain:41KF2a;

AJ831841

S001014162 - not_calculated 0.896 1443 Bacillus aerophilus (T); type strain:28K; AJ831844

> P43-36 10

GGGAACGTATTCACCGCGGCATTCTGATCCGCGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCC

AATCCGGACTACGATCGGCTTTTTGAGATTAGCATCCTATCGCTAGGTAGCAACCCTTTGTACCGACCATTGTAGCA

CGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCGTCCCCGCCTTCCTCCAGTTTGTCACTGGCAGTA

TCCTTAAAGTTCCCACCCAAAGTGCTGGCAAATAAGGAAAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCT

CACGACACGAGCTGACGACAGCCATGCAGCACCTGTATGTAAGTTCCCGAAGGCACCAATCCATCTCTGGAAAGTT

CTTACTATGTCAA

394 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GQ983193.1

Acinetobacter sp. C_AMB_IV2 16S ribosomal RNA

gene, partial sequence

711 711 100% 0.0 100%

GQ983192.1

Acinetobacter sp. C_AMB_II2 16S ribosomal RNA

gene, partial sequence

711 711 100% 0.0 100%

GQ983191.1

Acinetobacter sp. C_EMB_I2 16S ribosomal RNA

gene, partial sequence

711 711 100% 0.0 100%

Lineage:

Results for Query Sequence: P43-36, 384 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Moraxellaceae (5)

genus Acinetobacter (5)

S000001137 - not_calculated 0.969 1357 Acinetobacter johnsonii (T); DSM 6963; X81663

S000003452 - not_calculated 0.945 1366 Acinetobacter lwoffii (T); DSM 2403; X81665

S000008827 - not_calculated 0.969 1364 Acinetobacter haemolyticus (T); DSM 6962; X81662

S000127903 - not_calculated 1.000 1355 Acinetobacter ursingii (T); LUH 3792Type; AJ275038

S000381579 - not_calculated 0.951 1334 Acinetobacter calcoaceticus (T); ATCC 23055T; DNA

group one; Z93434

128

> P43-36 11

GGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAGGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

FJ894864.1

Uncultured bacterium clone nbt187a04 16S ribosomal

RNA gene, partial sequence

713 713 100% 0.0 100%

EU534924.1

Uncultured bacterium clone nbt187a04 16S ribosomal

RNA gene, partial sequence

713 713 100% 0.0 100%

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

708 708 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.964 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.982 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.982 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.967 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.982 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-36 12

GGGAACGTATTCACCGCGACATGCTGATCCGCGATTACTAGCGATTCCAACTTCATGCAGTCGAGTTGCAGACTAC

AATCCGGACTACGATACACTTTCTGCGATTAGCTCCCCCTCGCGGGTTGGCGGCGCTCTGTATGTACCATTGTATG

ACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCATTAGAGTGCCCTTTCGTAGCAACTAATGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACG

ACACGAGCTGACGACAGCCATGCAGCACCTGTGTACTGGTTCTCTTTCGAGCACTCCCTGATCTCTCAAGGATTCC

AGCCATGTCAA

390 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU213429.1

Janthinobacterium sp. 327 16S ribosomal RNA

gene, partial sequence

704 704 100% 0.0 100%

GU213422.1

Janthinobacterium sp. 287 16S ribosomal RNA

gene, partial sequence

704 704 100% 0.0 100%

GU213418.1

Janthinobacterium sp. 278 16S ribosomal RNA

gene, partial sequence

704 704 100% 0.0 100%

129

Lineage:

Results for Query Sequence: P43-36, 379 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Betaproteobacteria (5)

order Burkholderiales (5)

family Oxalobacteraceae (5)

genus Herbaspirillum (1)

S000002236 - not_calculated 0.876 1356 Herbaspirillum rubrisubalbicans (T); ATCC 19308T;

AB021424

genus Janthinobacterium (2)

S000006620 - not_calculated 0.960 1393 Janthinobacterium lividum (T); DSM 1522T; Y08846

S000008887 - not_calculated 0.918 1397 Janthinobacterium agaricidamnosum (T); W1r3T; Y08845

genus Herminiimonas (2)

S000574631 - not_calculated 0.881 1342 Herminiimonas fonticola (T); S-94; AY676462

S000592630 - not_calculated 0.913 1306 Herminiimonas aquatilis (T); type strain: CCUG 36956;

AM085762

> P43-36 13

GGGAACGTATTCACCCCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGCTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

704 704 100% 0.0 99%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

704 704 100% 0.0 99%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

704 704 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.951 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.969 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.969 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.954 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.969 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

130

> P43-36 14

GGGAACGTGTTCACCGCCACATTCTGATTCGCGATTACTAGCGATACCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU537964.1

Uncultured bacterium clone nbt228b07 16S ribosomal

RNA gene, partial sequence

704 704 100% 0.0 99%

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

699 699 100% 0.0 99%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

699 699 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-36, 388 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.930 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.948 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.948 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.933 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.948 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-36 15

GGGAACGTATTCACCGCCGACATTCTGATTTGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCATCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTACCCGAAGGCACCAATCTATCTCTAGAG

AGTTCATTGGATGTCAA

396 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU538276.1

Uncultured bacterium clone nbt84c01 16S ribosomal

RNA gene, partial sequence

693 693 100% 0.0 98%

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

688 688 100% 0.0 98%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

688 688 100% 0.0 98%

131

Lineage:

Results for Query Sequence: P43-36, 388 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000145156 - not_calculated 0.920 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.920 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366446 - not_calculated 0.905 1414 Pseudomonas meridiana (T); type strain: CMS 38;

AJ537602

S000366447 - not_calculated 0.905 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.920 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-36 16

GGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTATGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

708 708 100% 0.0 99%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

708 708 100% 0.0 99%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

708 708 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.964 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.982 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.982 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.967 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.982 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

132

> P43-36 17

GGCCCTGCCGTGTCACTCACTTTCCATCTCTGGCTTGGTGCCTTCGGGTCACTCGAACACAGGTGCTGCATGGCT

GTCGTCAGCTCGTGTCGTGAGCTGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCCTTAGTTGCCAGCACG

TAATGGTGGGAACTCTAAGGAGACCGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGC

CCTTACGGCCAGGGCTACACACGTACTACAATGGTAGGGACAGAGGGCTGCAAGCCGGCGACGGTAAGCCAATC

CCAGAAACCCTACCTCAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGCAGAT

CAGCATTGCTGCGGTGAATACG

396 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU816936.1

Uncultured Stenotrophomonas sp. clone l-35-2 16S

ribosomal RNA gene, partial sequence

643 643 100% 0.0 95%

GU254009.1

Stenotrophomonas sp. 2057 16S ribosomal RNA gene,

partial sequence

639 639 100%

3e-

180

95%

GQ268318.1

Stenotrophomonas maltophilia strain Y1 16S ribosomal

RNA gene, partial sequence

639 639 100%

3e-

180

95%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Xanthomonadales (5)

family Xanthomonadaceae (5)

genus Stenotrophomonas (5)

S000000936 not_calculated 0.859 1382 Pseudomonas pictorum (T); ATCC 23328T; AB021392

S000007629 not_calculated 0.866 1345 Pseudomonas geniculata (T); ATCC 19374T; AB021404

S000010261 not_calculated 0.861 1400 Pseudomonas hibiscicola (T); ATCC 19867T; AB021405

S000381870 not_calculated 0.869 1384 Stenotrophomonas maltophilia (T); ATCC 13637T;

AB008509

S000390459 not_calculated 0.835 1434 Stenotrophomonas acidaminiphila (T); AMX19; AF273080

> P43-36 18

GGGAACGTATTCACCGCGCCATTCTGATTCGCGATTACTAGCGATTCCGACTTCGCGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTCCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU539416.1

Uncultured bacterium clone nbt238e02 16S ribosomal

RNA gene, partial sequence

704 704 100% 0.0 99%

EU536354.1

Uncultured bacterium clone nbt61e08 16S ribosomal

RNA gene, partial sequence

704 704 100% 0.0 99%

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

699 699 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.933 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.949 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.949 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366446 - not_calculated 0.943 1414 Pseudomonas meridiana (T); type strain: CMS 38;

AJ537602

S000428331 - not_calculated 0.949 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

133

> P43-36 19

GGGAACGTATTCACCGTGACATTCTGATTCACGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTATGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTTTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCCCATTAGAGTGCTCAACTAAATGTAGCAACTAATGGCAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCT

CACGACACGGGCTGACGACAGCCATGCAGCACCTGTGTTACGGCTCTCTTTCGAGCACTCCTCTATCTCTAAAGGA

TTCCGTACATGTCAA

394 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GQ179675.1

Uncultured Comamonas sp. clone VE3A04 16S

ribosomal RNA gene, partial sequence

639 639 100%

3e-

180

95%

FJ893904.1

Uncultured bacterium clone nbt123d07 16S ribosomal

RNA gene, partial sequence

639 639 100%

3e-

180

95%

EU771850.1

Uncultured bacterium clone AFYEL_aaj68e12 16S

ribosomal RNA gene, partial sequence

639 639 100%

3e-

180

95%

Lineage:

Results for Query Sequence: P43-36, 385 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Betaproteobacteria (5)

order Burkholderiales (5)

family Comamonadaceae (5)

genus Comamonas (3)

S000127018 - not_calculated 0.769 1407 Comamonas nitrativorans (T); 23310; AJ251577

S000134317 - not_calculated 0.784 1247 Comamonas aquatica (T); type strain: LMG 2370;

AJ430344

S000428528 - not_calculated 0.779 1414 Comamonas terrigena (T); IMI 359870; AF078772

genus Acidovorax (1)

S000428522 - not_calculated 0.771 1413 Acidovorax temperans (T); CCUG 11779; AF078766

genus Giesbergeria (1)

S000610620 - not_calculated 0.779 1396 Giesbergeria kuznetsovii (T); D-412; AY780907

> P43-36 20

ACCTTTGACATGTACGGAATCCTTTAGAGATAGAGGAGCGCTCGAAAGAGAGCCGTAACACAGGTGCTGCATGGCT

GTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGCCATTAGTTGCTACATTTA

GTTGAGCACTCTAATGGGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTA

TAGGTGGGGCTACACACGTCATACAATGGCTGGTACAAAGGGTTGCCAACCCGCGAGGGGGAGCTAATCCCATAA

AGCCAGTCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGTGAAGTCGGAATCGCTAGTAATCGCGAATCAGAA

TGTCGCGGTGAATACG

393 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

GQ179675.1

Uncultured Comamonas sp. clone VE3A04 16S

ribosomal RNA gene, partial sequence

691 691 100% 0.0 98%

FJ893904.1

Uncultured bacterium clone nbt123d07 16S ribosomal

RNA gene, partial sequence

691 691 100% 0.0 98%

EU771850.1

Uncultured bacterium clone AFYEL_aaj68e12 16S

ribosomal RNA gene, partial sequence

691 691 100% 0.0 98%

134

Lineage:

Results for Query Sequence: P43-36, 382 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Betaproteobacteria (5)

order Burkholderiales (5)

family Comamonadaceae (5)

genus Comamonas (2)

S000134317 not_calculated 0.895 1247 Comamonas aquatica (T); type strain: LMG 2370;

AJ430344

S000428528 not_calculated 0.877 1414 Comamonas terrigena (T); IMI 359870; AF078772

genus Acidovorax (2)

S000009495 not_calculated 0.877 1319 Acidovorax avenae subsp. avenae (T); ATCC 19307T;

AB021421

S000428522 not_calculated 0.908 1413 Acidovorax temperans (T); CCUG 11779; AF078766

genus Ottowia (1)

S000263441 not_calculated 0.898 1427 Ottowia thiooxydans (T); type strain: K11; AJ537466

> P43-36 21

GGGAACGTATTCCCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGCG

ATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGCA

CGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGT

CTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACAT

CTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAAG

TTCATTGGATGTCGA

394 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU536049.1

Uncultured bacterium clone nbt07g09 16S ribosomal

RNA gene, partial sequence

704 704 100% 0.0 99%

EU535892.1

Uncultured bacterium clone nbt05h05 16S ribosomal

RNA gene, partial sequence

704 704 100% 0.0 99%

EU482924.1

Pseudomonas sp. CB21 16S ribosomal RNA gene,

partial sequence

702 702 99% 0.0 99%

Lineage:

Results for Query Sequence: P43-36, 388 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.964 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.982 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.982 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.966 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.982 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

135

> P43-36 22

GGCCTTGACATCCACTGAACTTTCTAGAGATAGATTGGTGCCTTCGGGAACATTGAGACAGGTGCTGCATGGCTGT

CGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTGTCCTTAGTTACCAGCACGTAA

TGGTGGGCACTCTAAGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTT

ACGGCCTGGGCTACACACGTGCTACAATGGTCGGTACAGAGGGTTGCCAAGCCGCGAGGTGGAGCTAATCCCAC

AAAACCGATCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGTGAAGTCGGAATCGCTAGTAATCGCGAATCAGA

ATGTCGCGGTGAATACG

394 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU536678.1

Uncultured bacterium clone nbt249b07 16S ribosomal

RNA gene, partial sequence

711 711 100% 0.0 100%

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

706 706 100% 0.0 99%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

706 706 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-36, 388 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 not_calculated 0.964 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 not_calculated 0.982 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 not_calculated 0.982 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 not_calculated 0.966 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 not_calculated 0.982 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-36 23

GGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGACTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCAGCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU537897.1

Uncultured bacterium clone nbt226h10 16S ribosomal

RNA gene, partial sequence

704 704 100% 0.0 99%

EU535207.1

Uncultured bacterium clone nbt172c08 16S ribosomal

RNA gene, partial sequence

704 704 100% 0.0 99%

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

699 699 100% 0.0 99%

136

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.928 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.946 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.946 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.931 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.946 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-36 24

GGGAACGTATTCACCGCGACATTCTGATTCACGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGTACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATATTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU535720.1

Uncultured bacterium clone nbt10a06 16S ribosomal

RNA gene, partial sequence

704 704 100% 0.0 99%

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

699 699 100% 0.0 99%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

699 699 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.941 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.959 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.959 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.943 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.959 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

137

> P43-36 25

GGGCACGTCTTCACCGCCCCATTCTGATTCGCGCTTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTCTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATAACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

FJ375458.1

Uncultured bacterium clone ACS45 16S ribosomal

RNA gene, partial sequence

690 690 100% 0.0 98%

GQ847629.1

Uncultured bacterium isolate DGGE gel band BAC7

16S ribosomal RNA gene, partial sequence

690 690 100% 0.0 98%

GQ847628.1

Uncultured bacterium isolate DGGE gel band BAC6

16S ribosomal RNA gene, partial sequence

690 690 100% 0.0 98%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000355057 - not_calculated 0.913 1299 Pseudomonas salomonii (T); CFBP 2022; AY091528

S000366447 - not_calculated 0.928 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000390380 - not_calculated 0.928 1401 Pseudomonas grimontii (T); CFML 97-514T; AF268029

S000428195 - not_calculated 0.928 1422 Pseudomonas libanensis (T); CIP 105460; AF057645

S000428466 - not_calculated 0.928 1415 Pseudomonas gessardii (T); CIP 105469; AF074384

> P43-36 26

GGGCACGTCTTCACCGCCCCATTCTGATTCGCGCTTACTAGCGCTTCCGACTTCACGCAGTCGAGTTGCATCCTGC

GCTCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACTTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

FJ893749.1

Uncultured bacterium clone nbt24f06 16S ribosomal

RNA gene, partial sequence

672 672 100% 0.0 97%

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

668 668 100% 0.0 97%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

668 668 100% 0.0 97%

Lineage:

Results for Query Sequence: P43-36, 388 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.858 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.876 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.876 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.861 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.876 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

138

> P43-36 27

GGGAACGTATTCACCGCCACATTCTGATTCTCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTATGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCCATCTCTGGAAA

GTTCATTGGATGTCAA

395 bases

Accession Description

Max

score

Total

score

Query

coverage

value

Max

ident

AB506067.1

Pseudomonas sp. StFRB218 gene for 16S rRNA,

partial sequence

704 704 100% 0.0 99%

GU086437.1

Pseudomonas sp. M3 16S ribosomal RNA gene,

partial sequence

704 704 100% 0.0 99%

GQ994885.1

Uncultured organism clone Jwat28 16S ribosomal

RNA gene, partial sequence

704 704 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000008929 - not_calculated 0.949 1433 Pseudomonas taetrolens (T); IAM1653; D84027

S000014863 - not_calculated 0.959 1375 Pseudomonas lundensis (T); ATCC 49968T; AB021395

S000395040 - not_calculated 0.964 1369 Pseudomonas umsongensis (T); Ps 3-10; AF468450

S000428381 - not_calculated 0.964 1398 Pseudomonas jessenii (T); CIP 105274; AF068259

S000428763 - not_calculated 0.949 1363 Pseudomonas chlororaphis subsp. aureofaciens (T);

ATCC 13985; AF094722

> P43-36 28

GGGAACGTATTCACCGCCACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGGCCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCGTGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCCATCTCTGGAA

AGTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EF142024.1

Uncultured gamma proteobacterium clone KF046 16S

ribosomal RNA gene, partial sequence

704 704 100% 0.0 99%

AY336121.1

Pseudomonas sp. SD-10 16S ribosomal RNA gene,

partial sequence

704 704 100% 0.0 99%

AB506069.1

Pseudomonas sp. StFRB234 gene for 16S rRNA,

partial sequence

699 699 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000108891 - not_calculated 0.946 1409 Pseudomonas kilonensis (T); 520-20; AJ292426

S000395042 - not_calculated 0.946 1366 Pseudomonas koreensis (T); Ps 9-14; AF468452

S000428332 - not_calculated 0.946 1422 Pseudomonas cedrina (T); CFML 96-198; AF064461

S000428886 - not_calculated 0.936 1383 Pseudomonas brassicacearum (T); DBK11; CFBP 11706;

AF100321

S000428888 - not_calculated 0.946 1342 Pseudomonas thivervalensis (T); SBK26; CFBP 11261T;

AF100323

139

> P43-36 29

GGGAACGTATCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGCG

ATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGCA

CGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGT

CTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACAT

CTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCTATGTTCCCGAAGGCACCAATCTATCTCTAGAAAG

TTCATTGGATGTCAA

394 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU630427.1

Uncultured Pseudomonas sp. clone URA1Q1u38_T3

16S ribosomal RNA gene, partial sequence

702 702 100% 0.0 99%

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

700 700 100% 0.0 99%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

700 700 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-36, 388 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.951 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.969 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.969 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.954 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.969 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-36 30

GGGAACGTATTCACCGCCACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

708 708 100% 0.0 99%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

708 708 100% 0.0 99%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

708 708 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.964 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.982 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.982 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.967 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.982 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

140

> P43-36 31

GGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTATGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198119.1

Pseudomonas fluorescens strain LMG 14571 16S

ribosomal RNA gene, partial sequence

713 713 100% 0.0 100%

GU198118.1

Pseudomonas fluorescens strain LMG 14566 16S

ribosomal RNA gene, partial sequence

713 713 100% 0.0 100%

GU198117.1

Pseudomonas fluorescens strain LMG 14562 16S

ribosomal RNA gene, partial sequence

713 713 100% 0.0 100%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000005898 - not_calculated 0.982 1429 Pseudomonas azotoformans (T); IAM1603; D84009

S000013342 - not_calculated 1.000 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145154 - not_calculated 1.000 1434 Pseudomonas poae (T); type strain: DSM 14936 = LMG

21465; P 527/13; AJ492829

S000145156 - not_calculated 0.982 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000499371 - not_calculated 0.985 1399 Pseudomonas costantinii (T); CFBP 5705; HAMBI 2444;

AF374472

> P43-36 32

GGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

713 713 100% 0.0 100%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

713 713 100% 0.0 100%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

713 713 100% 0.0 100%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.982 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 1.000 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 1.000 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.985 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 1.000 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

141

> P43-36 33

GGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

713 713 100% 0.0 100%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

713 713 100% 0.0 100%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

713 713 100% 0.0 100%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.982 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 1.000 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 1.000 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.985 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 1.000 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-36 34

GGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTCTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCTTTACGTGCTGGTAACTAAGGACAAGGGTTGCGTTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

699 699 100% 0.0 99%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

699 699 100% 0.0 99%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

699 699 100% 0.0 99%

142

Lineage:

Results for Query Sequence: P43-36, 387 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.953 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.953 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.953 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.948 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.953 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-36 35

GGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGC

GATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAGC

ACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACA

TCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTTTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAAA

GTTCATTGGATGTCAA

395 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

708 708 100% 0.0 99%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

708 708 100% 0.0 99%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

708 708 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-36, 389 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.967 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.985 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.985 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.969 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.985 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

143

> P43-36 36

GGGAACGTATCACCGCGGCATTCTGATCCGCGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCA

ATCCGGACTACGATCGGCTTTTTGAGATTAGCATCCTATCGCTAGGTAGCAACCCTTTGTACCGACCATTGTAGCAC

GTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCGTCCCCGCCTTCCTCCAGTTTGTCACTGGCAGTAT

CCTTAAAGTTCCCACCCGAAGTGCTGGCAAATAAGGAAAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTC

ACGACACGAGCTGACGACAGCCATGCAGCACCTGTATGTAAGTTCCCGAAGGCACCAATCCATCTCTGGAAAGTTC

TTACTATGTCAA

393 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

AM889158.1

Uncultured proteobacterium partial 16S rRNA gene,

clone 1'C12

702 702 100% 0.0 99%

FJ968638.1

Arcobacter sp. enrichment culture clone M4 16S

ribosomal RNA gene, partial sequence

702 702 100% 0.0 99%

FN432021.1

Uncultured bacterium partial 16S rRNA gene, isolate

w78

702 702 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-36, 382 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Moraxellaceae (5)

genus Acinetobacter (5)

S000001137 - not_calculated 0.955 1357 Acinetobacter johnsonii (T); DSM 6963; X81663

S000003452 - not_calculated 0.932 1366 Acinetobacter lwoffii (T); DSM 2403; X81665

S000008827 - not_calculated 0.955 1364 Acinetobacter haemolyticus (T); DSM 6962; X81662

S000127903 - not_calculated 0.971 1355 Acinetobacter ursingii (T); LUH 3792Type; AJ275038

S000381579 - not_calculated 0.937 1334 Acinetobacter calcoaceticus (T); ATCC 23055T; DNA

group one; Z93434

> P43-36 37

GGGAACGTGATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTG

CGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTAG

CACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA

GTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAAC

ATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGAA

AGTTCATTGGATGTCAA

396 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

706 706 100% 0.0 99%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

706 706 100% 0.0 99%

GU198116.1

Pseudomonas fluorescens strain LMG 7220 16S

ribosomal RNA gene, partial sequence

706 706 100% 0.0 99%

Lineage:

Results for Query Sequence: P43-36, 390 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.964 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.982 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.982 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.967 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.982 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

144

> P43-36 38

GGGAACGTGCTTCACCGCGACCATTCTGATTCGCGCTTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACT

GCGATCCGGACTACGATCGGTTTTGTGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTCTGTACCGACCATTGTA

GCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGC

AGTCTCCTTAGAGTGCCCACCATTACGTGCTGGTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAA

CATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAATGTTCCCGAAGGCACCAATCTATCTCTAGG

AAGTTCATTGGATGTCAA

397 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

EU537964.1

Uncultured bacterium clone nbt228b07 16S ribosomal

RNA gene, partial sequence

691 691 100% 0.0 98%

GU198121.1

Pseudomonas fluorescens strain LMG 14576 16S

ribosomal RNA gene, partial sequence

686 686 100% 0.0 98%

GU198120.1

Pseudomonas fluorescens strain LMG 14573 16S

ribosomal RNA gene, partial sequence

686 686 100% 0.0 98%

Lineage:

Results for Query Sequence: P43-36, 388 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Gammaproteobacteria (5)

order Pseudomonadales (5)

family Pseudomonadaceae (5)

genus Pseudomonas (5)

S000013342 - not_calculated 0.923 1429 Pseudomonas rhodesiae (T); CIP 104664; AF064459

S000145156 - not_calculated 0.941 1434 Pseudomonas trivialis (T); type strain: DSM 14937 = LMG

21464; P 513/19; AJ492831

S000366445 - not_calculated 0.941 1416 Pseudomonas antarctica (T); type strain: CMS 35;

AJ537601

S000366447 - not_calculated 0.928 1417 Pseudomonas proteolytica (T); type strain: CMS 64;

AJ537603

S000428331 - not_calculated 0.941 1420 Pseudomonas orientalis (T); CFML 96-170; AF064457

> P43-36 39

GGGAACGTCTTCACCGCGCCATGCTGATCCGCGATTACTAGCGATTCCAACTTCATGCAGTCGAGTTGCAGACTAC

AATCCGGACTACGATACACTTTCTGCGATTAGCTCCCCCTCGCGGGTTGGCGGCGCTCTGTATGTACCATTGTATG

ACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG

TCTCATTAGAGTGCCCTTTCGTAGCAACTAATGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACG

ACACGAGCTGACGACAGCCATGCAGCACCTGTGTACTGGTTCTCTTTCGAGCACTCCCTGATCTCTCAAGGATTCC

AGCCATGTCAA

390 bases

Accession

Description

Max

score

Total

score

Query

coverage

value

Max

ident

GU213429.1

Janthinobacterium sp. 327 16S ribosomal RNA

gene, partial sequence

695 695 100% 0.0 99%

GU213422.1

Janthinobacterium sp. 287 16S ribosomal RNA

gene, partial sequence

695 695 100% 0.0 99%

GU213418.1

Janthinobacterium sp. 278 16S ribosomal RNA

gene, partial sequence

695 695 100% 0.0 99%

145

Lineage:

Results for Query Sequence: P43-36, 378 unique oligos

no rank Root (5) (match sequences)

domain Bacteria (5)

phylum Proteobacteria (5)

class Betaproteobacteria (5)

order Burkholderiales (5)

family Oxalobacteraceae (5)

genus Herbaspirillum (1)

S000002236 - not_calculated 0.847 1356 Herbaspirillum rubrisubalbicans (T); ATCC 19308T;

AB021424

genus Janthinobacterium (2)

S000006620 - not_calculated 0.931 1393 Janthinobacterium lividum (T); DSM 1522T; Y08846

S000008887 - not_calculated 0.889 1397 Janthinobacterium agaricidamnosum (T); W1r3T; Y08845

genus Herminiimonas (2)

S000574631 - not_calculated 0.849 1342 Herminiimonas fonticola (T); S-94; AY676462

S000592630 - not_calculated 0.881 1306 Herminiimonas aquatilis (T); type strain: CCUG 36956;

AM085762

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